BIOCHEM ENGINEERING:
RECOMBINANT DNA TECH QUESTIONS
WITH 100% CORRECT ANSWERS {
GRADED A+ }
ways to cut DNA - ✔✔1. chemical cleavage (random)
2. shearing (random)
3. restriction enzymes
restriction endonucleases - ✔✔cut both strands of DNA duplex at a specific place
restriction mapping - ✔✔for determining the locations of restriction endonuclease
sites within a segment of DNA
sum of fragment sizes equals... - ✔✔size of unfragmented DNA molecule
, partial digestion - ✔✔DNA incubated with a restriction enzyme for a short time
so only fraction of cleavage site are cut
DNA ligase - ✔✔an enzyme that eventually joins the sugar-phosphate backbones
of the Okazaki fragments
DNA exonuclease - ✔✔chew back sticky ends to make them blunt
cloning vectors - ✔✔small piece of DNA used to introduce foreign gene into host
cell; used to get multiple copies of foreign genes
plasmid size - ✔✔10 kbp
expression vector - ✔✔plasmid that introduces gene of interest and aids in protein
product expression
artificial chromosomes - ✔✔BAC (300), YAC (1000), HAC (20,000)
plasmids contain... - ✔✔1. origin of replication
2. selectable markers
selectable markers - ✔✔genes encoded on plasmid that confer a growth advantage
origin of replication - ✔✔directs cellular machinery to copy the plasmid for
transmission to progeny
RECOMBINANT DNA TECH QUESTIONS
WITH 100% CORRECT ANSWERS {
GRADED A+ }
ways to cut DNA - ✔✔1. chemical cleavage (random)
2. shearing (random)
3. restriction enzymes
restriction endonucleases - ✔✔cut both strands of DNA duplex at a specific place
restriction mapping - ✔✔for determining the locations of restriction endonuclease
sites within a segment of DNA
sum of fragment sizes equals... - ✔✔size of unfragmented DNA molecule
, partial digestion - ✔✔DNA incubated with a restriction enzyme for a short time
so only fraction of cleavage site are cut
DNA ligase - ✔✔an enzyme that eventually joins the sugar-phosphate backbones
of the Okazaki fragments
DNA exonuclease - ✔✔chew back sticky ends to make them blunt
cloning vectors - ✔✔small piece of DNA used to introduce foreign gene into host
cell; used to get multiple copies of foreign genes
plasmid size - ✔✔10 kbp
expression vector - ✔✔plasmid that introduces gene of interest and aids in protein
product expression
artificial chromosomes - ✔✔BAC (300), YAC (1000), HAC (20,000)
plasmids contain... - ✔✔1. origin of replication
2. selectable markers
selectable markers - ✔✔genes encoded on plasmid that confer a growth advantage
origin of replication - ✔✔directs cellular machinery to copy the plasmid for
transmission to progeny
