CMB LAB EXAM #1
aseptic technique - ✅✅ -method used to make the environment, the
worker, and the patient as germ-free as possible
simple stain - ✅✅-a method of staining microorganisms with a single
basic dye
differential stain -✅✅ -technique to distinguish two or more types of
microorganisms based on physical
and/or chemical reactions with reagents used
Gram stain - ✅✅ -- uses bacterial cell wall differences to differentiate
groups of bacteria
- crystal violet is primary stain
- lugol's iodine is fixing reagent
- alcohol is decolorizing agent
- safranin is counterstain
- gram positive is purple bc of thick peptidoglycan
- gram negative is pink
acid fast stain - ✅✅ -- a differential stain used to identify bacteria with
high mycolid acid content
- acid fast bacteria is resistant ti decolorization by 3% HCL during
staining
- carbol fuschin is primary stain
- decolorized with 3% HCL
- acid-fast cells stain red
- methylene blue is counter stain
- non-acid-fast cells stain blue
mycolic acids - ✅✅-long-chain fatty acids that are found in the cell wall
of mycobacterium
endospore stain - ✅✅ -- malachite green is primary stain, driven into
cells and endospores w heat, water soluble so will rinse everything off
but spores
- safranin is counterstain
aseptic technique - ✅✅ -method used to make the environment, the
worker, and the patient as germ-free as possible
simple stain - ✅✅-a method of staining microorganisms with a single
basic dye
differential stain -✅✅ -technique to distinguish two or more types of
microorganisms based on physical
and/or chemical reactions with reagents used
Gram stain - ✅✅ -- uses bacterial cell wall differences to differentiate
groups of bacteria
- crystal violet is primary stain
- lugol's iodine is fixing reagent
- alcohol is decolorizing agent
- safranin is counterstain
- gram positive is purple bc of thick peptidoglycan
- gram negative is pink
acid fast stain - ✅✅ -- a differential stain used to identify bacteria with
high mycolid acid content
- acid fast bacteria is resistant ti decolorization by 3% HCL during
staining
- carbol fuschin is primary stain
- decolorized with 3% HCL
- acid-fast cells stain red
- methylene blue is counter stain
- non-acid-fast cells stain blue
mycolic acids - ✅✅-long-chain fatty acids that are found in the cell wall
of mycobacterium
endospore stain - ✅✅ -- malachite green is primary stain, driven into
cells and endospores w heat, water soluble so will rinse everything off
but spores
- safranin is counterstain
