Leaving Cert Biology: Genetic
Engineering
genetic engineering
artificial manipulation and alteration of genes
process of genetic engineering
1. Isolation
2. Cutting and Ligation
3. Transformation/transfection/transduction
4. Selection and cloning
5. Expression
1. Isolation
process of removing Gene from cells.
2. cutting
removal of a gene from a piece of DNA using a restriction enzyme.
vector
piece of DNA that will carry a gene of interest into a host cell.
Name the rings of DNA contained in bacteria that can be used as cloning vectors
Plasmids
2. ligation
joining of a gene to a vector using DNA ligase.
Recombinant DNA
piece of genetically modified DNA that contains DNA from two or more different species.
3. Transformation/transfection/transduction:
is the term used to generally describe the uptake of recombinant DNA into a host cell.
transformation
uptake of recombinant DNA into a bacterial cell
Engineering
genetic engineering
artificial manipulation and alteration of genes
process of genetic engineering
1. Isolation
2. Cutting and Ligation
3. Transformation/transfection/transduction
4. Selection and cloning
5. Expression
1. Isolation
process of removing Gene from cells.
2. cutting
removal of a gene from a piece of DNA using a restriction enzyme.
vector
piece of DNA that will carry a gene of interest into a host cell.
Name the rings of DNA contained in bacteria that can be used as cloning vectors
Plasmids
2. ligation
joining of a gene to a vector using DNA ligase.
Recombinant DNA
piece of genetically modified DNA that contains DNA from two or more different species.
3. Transformation/transfection/transduction:
is the term used to generally describe the uptake of recombinant DNA into a host cell.
transformation
uptake of recombinant DNA into a bacterial cell
