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Microbiology Exam 2 Questions and answers.

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Microbiology Exam 2 Questions and answers.

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Microbiology Exam 2 Questions and
answers
Are Viruses Living or Nonliving? - correct answer-They are only considered living when they
are inside of a host cell, using their resources and energy.

Outside of a host they are considered inert.

When are Viruses considered to be "alive"? - correct answer-When they are inside of a host
cell.

When inside, their nucleic acid (ONLY RNA OR DNA) become active.
*Virus replicates

Obligatory Intracellular Parasites - correct answer-Require livinghost cell in order to multiply -
Such as Viruses.

Defining a Virus - correct answer-1) Contains 1 Nucleic Acid (DNA or RNA)
2) Protein coat surrounds the DNA or RNA
3)Multiply within host using host machinery
---because they don't have the ways of making/obtaining their own nutrients. like ATP or
enzymes.

Virus Characteristics - correct answer-*Some are enclosed by an envelope which consists of
lipids, proteins, carbohydrates

*some have spikes

*only infect the cells of ONE host.

Host Range - correct answer-The spectrum of host cells that a virus can infect.
-invertebrates
-vertebrates
-plants
-protist
-fungi
-bacteria

The virus must be able to attach to a the particular host's cell.

**Host range is determined b specific attachment sites on the host cell's surface

Bacteriophages - correct answer-Bacteria that is infected by a virus

,How do Viruses attach to a host cell - correct answer-Through receptors on the host cell's
surface.
There must be an interaction.

Capsid - correct answer-Protein coat that surrounds the Nucleic Acid of a virus. (not the
whole virus itself. That's an envelope.)

****Made up of individualized Capsomeres****

*accounts for most of the mass of a viral cell.

Capsomeres - correct answer-Protein subunits that make up a capsid

(think of tiny balls that come together to make a huge coating around the Nucleic Acid)

*capsomeres can be made up of multiple types of proteins or one single type of protein

Envelope - correct answer-Surrounds the virus as a whole.
*Synonymus to a somatic cell's Plasma Membrane. But around a virus.

***Composed of proteins, lipids and carbs.

*Could be covered in spikes

NOT ALL VIRUSES HAVE ENVELOPES

Spikes - correct answer-Cover the envelope of the cell
Composed of protein and carbohydrates.

-Used for attachment to the host cell

Spikes differ, and can be identified as different virus by the host body. That's why you can
get the flu more than once a year, there's a change in spikes.
-Can be used for identification for some virsus under microscope.

Helical Capsid - correct answer-Long rods that are flexible and rigid

These surround the Nucleic Acid.

*Capsids are hollow cylinders surrounding the nucleic acid.

*CAN OR CANNOT HAVE A ENVELOPE.

Without envelope = they look like long noodles, because it's just the capsid and no
sphereical shape
WITH the envelope = they look like balls, but with their Nucleic acids all tangled inside.

, Polyhedrical Capsid - correct answer-These are icosahedron, and are 20 triangle shapes,
with 12 corners.

THEY CAN BE WITH OR WITHOUT ENVELOPE
-Without envelope, you can see their individual caposomere and their many different
triangular faces of the Capsid

WITH envelope, they appear ordinarily spherical, and like a normal cell. (Some have spikes)

Complex Capsid - correct answer-They have a variety of weird shapes within the one virus
cell, and look just straight up weird.

They can be bacteriaphages and just look mutuant

Family names suffix for Taxonomy - correct answer--viridae

Ex. Herpesviridae

Genus name suffix Taxonomy - correct answer--virus

Genus Simplexvirus
Ex. Herpesvirus

Viral species - correct answer-A group of viruses sharing the same genetic information and
ecological niche (niche=host)


Viral species are designated by descriptive common names, such as human
immunodeficiency virus (HIV),

Subspecies - correct answer-They are determined by the NUMBER that is attached to the
specific genus of the family. These are viruses that can affect humans

HIV-1, HIV-2

Plaque - correct answer-Areas of clearing where the the virus has killed the bacteria

PFU - correct answer-Plaque Forming Unit

*Concentrations of viral suspensions measured by the number of plaques

Cytopathic Effect - correct answer-CPE

This is cell deterioration after a virus infects a monolayer of cells that appears to be growing
just fine.

The cells clump together as they deteriorate.

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