Mo Bio Final - Exam 1
A deoxyribonucleotide is composed of:
A. a nitrogenous base and a deoxyribose
B. a nitrogenous base, a deoxyribose, and a phosphate group
C. a nitrogenous base, a ribose, and a phosphate group
D. a nitrogenous base and a phosphate group - correct answer-B. a nitrogenous base, a
deoxyribose, and a phosphate group
A heterozygous organism has two identical copies of an allele for a gene.
A. True
B. False - correct answer-B. False
A plasmid vector typically has all of the following features EXCEPT:
A. origin of replication
B. selectable marker
C. unique restriction sites
D. a size range from 4-15 kbp
E. par genes - correct answer-E. par genes
A polylinker is a:
A. synthetic DNA fragment that contains multiple restriction enzymes sites
B. synthetic DNA fragment inserted into a vector that allows replication of the vector in the
host cell
C. DNA sequence that allows for selection of cells that contains the vector
D. molecule used to ligate DNA fragments together
E. synthetic DNA fragment used in colony hybridization to visualize colonies that contain the
DNA of interest - correct answer-A. synthetic DNA fragment that contains multiple restriction
enzymes sites
A recombinant DNA was constructed by inserting the DNA of interest into the AmpR gene of
a pBR322 vector. The vector also contains a TetR gene that is not interrupted. After
introducing the recombinant DNA into bacterial cells, the resulting transformants were tested
on a variety of media. Which of the following results would be expected?
A. growth on both ampicillin containing media and tetracycline containing media
B. growth on ampicillin but not tetracycline containing media
C. growth on tetracycline but not ampicillin containing media
D. no growth on either ampicillin or tetracycline containing media
E. growth only on media containing both antibiotics - correct answer-C. growth on
tetracycline but not ampicillin containing media
A student clones a DNA insert into the SalI site of pBR322. Which answer describes the
media on which she could expect the growth of E. coli containing the recombinant plasmid.
(picture not shown)
A. nutrient agar with ampicillin
B. nutrient agar with tetracyline
, C. nutrient agar with no antibiotics
D. all of the above
E. A and C - correct answer-E. A and C
Chaperones:
A. bind improperly folded peptides and return them to their functional state
B. are heat shock proteins
C. encourage teenagers to make physical contact at the prom
D. all of the above
E. A and B - correct answer-E. A and B
Crispr/Cas:
A. is a new type breakfast cereal
B. is derived from bacterial bacteriphage defense systems
C. operates to cleave DNA specifically using a complex of crispr guide RNA and Cas
nuclease
D. all of the above
E. B and C - correct answer-E. B and C
DNA is cleaved by various enzymes to generate DNA fragments of sizes 543 bp, 517 bp,
396 bp, and 214 bp. Which fragment will move the fastest in aragose gel electrophoresis?
A. 543 bp
B. 517 bp
C. 396 bp
D. 214 bp - correct answer-D. 214 bp
DNA ligase:
A. cleaves DNA at specific sites
B. requires cofactors
C. creates phosphodiester bonds
D. all of the above
E. B and C - correct answer-E. B and C
DNA methylation:
A. increases levels of gene expression
B. is increased in cancers\
C. is more highly to occur in CpG sequences
D. all of the above
E. B and C - correct answer-E. B and C
DNA microarrarys:
A. can reveal protein-protein interactions.
B. demonstrate differential expression of genes
C. can reveal protein-RNA interactions
D. can be used to purify proteins that bind to epitope tags
E. are somewhat large and unwieldy but give a great deal of information - correct answer-B.
demonstrate differential expression of genes
A deoxyribonucleotide is composed of:
A. a nitrogenous base and a deoxyribose
B. a nitrogenous base, a deoxyribose, and a phosphate group
C. a nitrogenous base, a ribose, and a phosphate group
D. a nitrogenous base and a phosphate group - correct answer-B. a nitrogenous base, a
deoxyribose, and a phosphate group
A heterozygous organism has two identical copies of an allele for a gene.
A. True
B. False - correct answer-B. False
A plasmid vector typically has all of the following features EXCEPT:
A. origin of replication
B. selectable marker
C. unique restriction sites
D. a size range from 4-15 kbp
E. par genes - correct answer-E. par genes
A polylinker is a:
A. synthetic DNA fragment that contains multiple restriction enzymes sites
B. synthetic DNA fragment inserted into a vector that allows replication of the vector in the
host cell
C. DNA sequence that allows for selection of cells that contains the vector
D. molecule used to ligate DNA fragments together
E. synthetic DNA fragment used in colony hybridization to visualize colonies that contain the
DNA of interest - correct answer-A. synthetic DNA fragment that contains multiple restriction
enzymes sites
A recombinant DNA was constructed by inserting the DNA of interest into the AmpR gene of
a pBR322 vector. The vector also contains a TetR gene that is not interrupted. After
introducing the recombinant DNA into bacterial cells, the resulting transformants were tested
on a variety of media. Which of the following results would be expected?
A. growth on both ampicillin containing media and tetracycline containing media
B. growth on ampicillin but not tetracycline containing media
C. growth on tetracycline but not ampicillin containing media
D. no growth on either ampicillin or tetracycline containing media
E. growth only on media containing both antibiotics - correct answer-C. growth on
tetracycline but not ampicillin containing media
A student clones a DNA insert into the SalI site of pBR322. Which answer describes the
media on which she could expect the growth of E. coli containing the recombinant plasmid.
(picture not shown)
A. nutrient agar with ampicillin
B. nutrient agar with tetracyline
, C. nutrient agar with no antibiotics
D. all of the above
E. A and C - correct answer-E. A and C
Chaperones:
A. bind improperly folded peptides and return them to their functional state
B. are heat shock proteins
C. encourage teenagers to make physical contact at the prom
D. all of the above
E. A and B - correct answer-E. A and B
Crispr/Cas:
A. is a new type breakfast cereal
B. is derived from bacterial bacteriphage defense systems
C. operates to cleave DNA specifically using a complex of crispr guide RNA and Cas
nuclease
D. all of the above
E. B and C - correct answer-E. B and C
DNA is cleaved by various enzymes to generate DNA fragments of sizes 543 bp, 517 bp,
396 bp, and 214 bp. Which fragment will move the fastest in aragose gel electrophoresis?
A. 543 bp
B. 517 bp
C. 396 bp
D. 214 bp - correct answer-D. 214 bp
DNA ligase:
A. cleaves DNA at specific sites
B. requires cofactors
C. creates phosphodiester bonds
D. all of the above
E. B and C - correct answer-E. B and C
DNA methylation:
A. increases levels of gene expression
B. is increased in cancers\
C. is more highly to occur in CpG sequences
D. all of the above
E. B and C - correct answer-E. B and C
DNA microarrarys:
A. can reveal protein-protein interactions.
B. demonstrate differential expression of genes
C. can reveal protein-RNA interactions
D. can be used to purify proteins that bind to epitope tags
E. are somewhat large and unwieldy but give a great deal of information - correct answer-B.
demonstrate differential expression of genes
