MICRO FINAL STUDY GUIDE WITH
QUESTIONS AND ANSWERS {
GUARANTEED A+}
describe the following safety procedures: proper PPE, handwashing,
disinfection of lab bench and equipment, proper labeling and storage of
media - ✔✔Proper PPE (Personal Protective Equipment):
Description: PPE includes items such as lab coats, gloves, safety
goggles, and face shields. These are essential to protect the skin, eyes,
and clothing from hazardous substances.
Usage: Always wear a lab coat to protect your clothing and skin. Gloves
should be worn when handling chemicals, biological materials, or any
,potentially hazardous substances. Safety goggles or face shields are
necessary when there is a risk of splashes or airborne particles.
Handwashing:
Description: Handwashing is a critical practice to prevent
contamination and the spread of pathogens.
Procedure: Wash hands thoroughly with soap and water for at least 20
seconds. This should be done before and after handling any materials,
after removing gloves, and before leaving the lab.
Disinfection of Lab Bench and Equipment:
Description: Disinfection involves using chemical agents to eliminate or
reduce harmful microorganisms on surfaces and equipment.
Procedure: Use an appropriate disinfectant (e.g., 70% ethanol, bleach
solution) to wipe down lab benches and equipment before and after
use. Ensure that the disinfectant is left on the surface for the
recommended contact time to be effective.
,Proper Labeling and Storage of Media:
Description: Proper labeling and storage are crucial for maintaining the
integrity of media and ensuring safety.
Procedure: Label all media with relevant information such as the name
of the media, date of preparation, and any specific storage conditions.
Store media in appropriate conditions (e.g., refrigeration, room
temperature) as specified by the manufacturer or protocol.
List and describe BSL levels. Which do we work with? - ✔✔BSL-1
(Biosafety Level 1):
Description: This is the lowest level of biosafety and is suitable for work
with well-characterized agents that are not known to cause disease in
healthy humans. Standard microbiological practices are sufficient.
Examples: Non-pathogenic strains of E. coli.
BSL-2 (Biosafety Level 2):
, Description: This level is for work with agents that pose moderate
hazards to personnel and the environment. It includes all BSL-1
practices plus additional precautions such as limited access to the lab
and the use of PPE.
Examples: Staphylococcus aureus, Salmonella spp.
BSL-3 (Biosafety Level 3):
Description: This level is for work with agents that can cause serious or
potentially lethal diseases through inhalation. It requires controlled
access, specialized ventilation systems, and the use of PPE.
Examples: Mycobacterium tuberculosis, SARS-CoV-2.
BSL-4 (Biosafety Level 4):
Description: This is the highest level of biosafety and is for work with
dangerous and exotic agents that pose a high risk of life-threatening
disease. It requires full-body, air-supplied suits, and specialized
facilities.
QUESTIONS AND ANSWERS {
GUARANTEED A+}
describe the following safety procedures: proper PPE, handwashing,
disinfection of lab bench and equipment, proper labeling and storage of
media - ✔✔Proper PPE (Personal Protective Equipment):
Description: PPE includes items such as lab coats, gloves, safety
goggles, and face shields. These are essential to protect the skin, eyes,
and clothing from hazardous substances.
Usage: Always wear a lab coat to protect your clothing and skin. Gloves
should be worn when handling chemicals, biological materials, or any
,potentially hazardous substances. Safety goggles or face shields are
necessary when there is a risk of splashes or airborne particles.
Handwashing:
Description: Handwashing is a critical practice to prevent
contamination and the spread of pathogens.
Procedure: Wash hands thoroughly with soap and water for at least 20
seconds. This should be done before and after handling any materials,
after removing gloves, and before leaving the lab.
Disinfection of Lab Bench and Equipment:
Description: Disinfection involves using chemical agents to eliminate or
reduce harmful microorganisms on surfaces and equipment.
Procedure: Use an appropriate disinfectant (e.g., 70% ethanol, bleach
solution) to wipe down lab benches and equipment before and after
use. Ensure that the disinfectant is left on the surface for the
recommended contact time to be effective.
,Proper Labeling and Storage of Media:
Description: Proper labeling and storage are crucial for maintaining the
integrity of media and ensuring safety.
Procedure: Label all media with relevant information such as the name
of the media, date of preparation, and any specific storage conditions.
Store media in appropriate conditions (e.g., refrigeration, room
temperature) as specified by the manufacturer or protocol.
List and describe BSL levels. Which do we work with? - ✔✔BSL-1
(Biosafety Level 1):
Description: This is the lowest level of biosafety and is suitable for work
with well-characterized agents that are not known to cause disease in
healthy humans. Standard microbiological practices are sufficient.
Examples: Non-pathogenic strains of E. coli.
BSL-2 (Biosafety Level 2):
, Description: This level is for work with agents that pose moderate
hazards to personnel and the environment. It includes all BSL-1
practices plus additional precautions such as limited access to the lab
and the use of PPE.
Examples: Staphylococcus aureus, Salmonella spp.
BSL-3 (Biosafety Level 3):
Description: This level is for work with agents that can cause serious or
potentially lethal diseases through inhalation. It requires controlled
access, specialized ventilation systems, and the use of PPE.
Examples: Mycobacterium tuberculosis, SARS-CoV-2.
BSL-4 (Biosafety Level 4):
Description: This is the highest level of biosafety and is for work with
dangerous and exotic agents that pose a high risk of life-threatening
disease. It requires full-body, air-supplied suits, and specialized
facilities.
