Research Article
EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF MARKETED
HERBAL DRUG PREPARATION
ABSTRACT
The aim of the present study is to evaluate the protective effect of marketed herbal preparation (Duliv) against carbon
tetrachloride (CCl4) induced liver damage in Albino rats. Administration with Duliv Tablet (100mg/kg p.o.)and Duliv Syrup
0.64 ml/100 gm for 15 days significantly reduced the impact of CCl4 toxicity on the serum markers of liver damage,
SGPT,SGOT, albumin,protein , cholesterol and alkaline phosphatase.The histopathological studies in the liver of rats
also supported that Duliv tablet and Duliv syrup markedly reduced the toxicity of CCl4 and preserved the histoarchitecture
of the liver tissue to near normal. Thus, the results suggest that Duliv preparation acts as a potent hepatoprotective
agent against CCl4 induced hepatotoxicity in rats.
Keywords: Duliv tablet, Carbon tetrachloride, SGPT, SGOT, Hepatoprotective.
INTRODUCTION Liver damage is not easy to detect early by an existing
testing methods. This is one of the most important
The liver cell is one of the few types of cell of the body obstacles in the research progress regarding liver.
which carries important function. It is involved in the Secondly the response of the liver to any damaging
process of metabolism of proteins, carbohydrates and factor does not vary. It always culminates into
the fats. It is of prime importance in bilirubin Cirrhosis or the Fibrosis which replaces the injured or
metabolism and secretes conjugated bilirubin into the dead hepatocytes. This makes difficult to assess the
bile. It has a role in the detoxification of harmful effect of the drug. A number of etiological factors thus
endogenous toxins e.g. Ammonia and purines and the ultimately culminate into end stage liver disease that
exogenous toxins such as various drugs. is known as cirrhosis.
Liver disease may be considered as being either diffuse Right from alcohol to drugs and viruses virtually many
or focal. In diffuse or generalized liver disease e.g. things can damage the liver cells if the challenge
hepatitis or bile duct obstruction, impaired liver function continues to attack the organ. Different toxic
is usually an early manifestation and may result in substances from viruses and bacteria, to the artificial
Jaundice. Acute liver failure, fluid retention, amoebic food, flavours, colours, preservatives and antibiotics,
abscess or there may be multiple lesions. E.g. metastatic these are the number of substances capable of
tumour in this focal disease, interference with overall producing the acute as well as chronic liver damage.
liver functions may be in a late complication.1 The present treatment of the liver diseases according
The problem of liver damage in both acute as well as to modern allopathic medicines is not very satisfactory
chronic, continue to trouble the medical profession which advocates only, rest and fat free diet and leaves
throughout the world as even today we have very little everything to the liver to regenerate by itself. There
knowledge about the exact mode of functioning of the is no specific therapy, in fact for the prevention as well
liver. We have lot of knowledge about the enzymes which as the cure of the acute and chronic liver diseases.
the liver holds in its parenchyma and many chemical Hence there is an ever increasing need for an agent
reactions going on in this organ but with all which could protect against the liver damage. Up till
now, no correct medicine has been we available from
*Corresponding author: synthetic drugs. Hence the reliance is given only on
Dr. Jangme C.M.
Ayurveda Medicine for the treatment of liver diseases
MaharashtraCollege of Pharmacy,
Nilanga, Dist: Latur, Maharashtra, India and disorder.3In this way the liver preparations
mentioned in Ayurvedic treatise or authoritative books
this knowledge we cannot accurately predict either have a special importance in medicines. It would be a
the recovery or the damage in the liver. This is because valued interest to find out the efficacy of this liver
in the liver the damage and the repair is going on side preparation in different, induced liver diseases and
by side and the organ has great reserves. So that even disorder, wherever possible in animals models. In
if seven parts out of the eight of the liver is damaged present study, evaluation of hepatoprotective
even then the liver is in a position to carry on the preparation mentioned in Ayurveda is attempted on
normal physiological functions without any external modern lines to test its claimed effects in liver disorders.
clinical signs detectable by the physician.2 Plan of Work:
Universal Journal of Pharmacy, 06(02), March-April 2017 8
, Hepatocellular disease is known to be associated with C) Biochemical Parameters:
impaired hepatic drug metabolising capacity and 1. Serum glutamate oxaloacetate Transaminase (SGOT)
impaired activity of hepatic enzymes. Liver diseases are 2. Serum glutamate pyruvate Transaminase (SGPT)
of different aetiology and till do day the best remedy is 3. Alkaline Phosphatase.
from natural drugs only. Ayurvedic books have prescribed 4. Serum bilirubin
as number of formulations in the treatment of liver 5. Cholesterol
diseases. 6. Total Proteins
A number of indigenous formulations have been claimed 7. Albumin
to possess hepatoprotective activity. A few of the have 8. Globulin.
also been studied in experimental models. It will be of D) Histological changes Such as:
great value if all such formulations are biologically 1. Fatty degeneration 2.
Table 1: Active ingredient with their quantity of both formulations
Each Sugar coated Duliv Syrup
Common name Biological Name tablet contains Each ml contains.
(Formulation A) (Formulation B)
Sharpunkha TephrosiaPurpurea 150mg 200 mg
BhuiAvla Phyllanthusnuriri 40mg 50 mg
Maka Eclipta alba 40mg 50 mg
Sunth ZingiberOfficinale 10mg 25 mg
Tulas Ocimum Sanctum 10mg 25 mg
Hirda TerminaliaChebula 30mg 50 mg
Gulvel Tenosporacordifolia 20 mg 25 mg
Kalmegh AndrographisPeniculata 50 mg 20 mg
Punarnava Boerhaaviadiffussa 50 mg 100 mg
assessed on animal models, wherever possible, and also Focci of Necrosis
by clinical trials. This will be revealing the utility of 3. Congestions.
these formulations in liver diseases of varying aetiology. E) The statistical Significance.
“DULIV” Is an indigenous herbal formulation which is The overall objectives of these methods to be applied is
claimed to be hepatoprotective. It contains nine to prepared a monograph for the above mentioned
indigenous herbs which have been mentioned in hepatoprotective formulation.
Ayurveda as hepatoprotective.
In the present investigation effect of “DULIV TABLET” MATERIALS AND METHODS
and “DULIV SYRUP”prepared by incorporating well “DULIV”an indigenous herbal formulation contains nine
known indigenous plants was studied in Carbon indigenous herbs which have been mentioned in
tetrachloride induced liver damage in Albino Rats. The Ayurveda as hepatoprotective. However no scientific
entire experimentation was carried out in the following study of the formulation prepared from these plants has
way: been reported.
1. Procurement of drugs of the above mentioned The present study has been undertaken to evaluate the
formulation. role of DULIV as a function of time on the toxic effect
2. Determination of safe dose of these formulations by induced by CCL4challenge on Serum enzyme. DULIV was
using Albino mice. obtained as: Formulation ‘A’( Duliv Tablet)
3. Induction of hepatotoxicity in albino rats by using Formulation ‘B’( Duliv Surup)
CCL4. Tablet was crushed and the powder (100mg/kg p.o. daily
4. Treatment of toxicity by administering formulation for 15days) was given as freshly prepared suspension in
separately in different groups of albino rats. gum acacia. Control animals received only the vehicle.
5. Assessment of hepatoprotective effect by using the The literature survey has revealed that the
parameters as given below and subsequent entireingredients in this formulation are individually
comparison. having hepatoprotective activity. Determination of Safe
A) Functional Paramter. Dose (Toxicity studies): Duliv preparation was studied
1. Measurment of pentobarbitone sleeping time. B) for its hepatoprotective activity. Prior to determine this
Morphological Paramenters. effect the safe dose of the formulation A and
1. Weight of Liver. Formulation B was found out.
2. Volume of Liver.
Universal Journal of Pharmacy, 06(02), March-April 2017 9
EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF MARKETED
HERBAL DRUG PREPARATION
ABSTRACT
The aim of the present study is to evaluate the protective effect of marketed herbal preparation (Duliv) against carbon
tetrachloride (CCl4) induced liver damage in Albino rats. Administration with Duliv Tablet (100mg/kg p.o.)and Duliv Syrup
0.64 ml/100 gm for 15 days significantly reduced the impact of CCl4 toxicity on the serum markers of liver damage,
SGPT,SGOT, albumin,protein , cholesterol and alkaline phosphatase.The histopathological studies in the liver of rats
also supported that Duliv tablet and Duliv syrup markedly reduced the toxicity of CCl4 and preserved the histoarchitecture
of the liver tissue to near normal. Thus, the results suggest that Duliv preparation acts as a potent hepatoprotective
agent against CCl4 induced hepatotoxicity in rats.
Keywords: Duliv tablet, Carbon tetrachloride, SGPT, SGOT, Hepatoprotective.
INTRODUCTION Liver damage is not easy to detect early by an existing
testing methods. This is one of the most important
The liver cell is one of the few types of cell of the body obstacles in the research progress regarding liver.
which carries important function. It is involved in the Secondly the response of the liver to any damaging
process of metabolism of proteins, carbohydrates and factor does not vary. It always culminates into
the fats. It is of prime importance in bilirubin Cirrhosis or the Fibrosis which replaces the injured or
metabolism and secretes conjugated bilirubin into the dead hepatocytes. This makes difficult to assess the
bile. It has a role in the detoxification of harmful effect of the drug. A number of etiological factors thus
endogenous toxins e.g. Ammonia and purines and the ultimately culminate into end stage liver disease that
exogenous toxins such as various drugs. is known as cirrhosis.
Liver disease may be considered as being either diffuse Right from alcohol to drugs and viruses virtually many
or focal. In diffuse or generalized liver disease e.g. things can damage the liver cells if the challenge
hepatitis or bile duct obstruction, impaired liver function continues to attack the organ. Different toxic
is usually an early manifestation and may result in substances from viruses and bacteria, to the artificial
Jaundice. Acute liver failure, fluid retention, amoebic food, flavours, colours, preservatives and antibiotics,
abscess or there may be multiple lesions. E.g. metastatic these are the number of substances capable of
tumour in this focal disease, interference with overall producing the acute as well as chronic liver damage.
liver functions may be in a late complication.1 The present treatment of the liver diseases according
The problem of liver damage in both acute as well as to modern allopathic medicines is not very satisfactory
chronic, continue to trouble the medical profession which advocates only, rest and fat free diet and leaves
throughout the world as even today we have very little everything to the liver to regenerate by itself. There
knowledge about the exact mode of functioning of the is no specific therapy, in fact for the prevention as well
liver. We have lot of knowledge about the enzymes which as the cure of the acute and chronic liver diseases.
the liver holds in its parenchyma and many chemical Hence there is an ever increasing need for an agent
reactions going on in this organ but with all which could protect against the liver damage. Up till
now, no correct medicine has been we available from
*Corresponding author: synthetic drugs. Hence the reliance is given only on
Dr. Jangme C.M.
Ayurveda Medicine for the treatment of liver diseases
MaharashtraCollege of Pharmacy,
Nilanga, Dist: Latur, Maharashtra, India and disorder.3In this way the liver preparations
mentioned in Ayurvedic treatise or authoritative books
this knowledge we cannot accurately predict either have a special importance in medicines. It would be a
the recovery or the damage in the liver. This is because valued interest to find out the efficacy of this liver
in the liver the damage and the repair is going on side preparation in different, induced liver diseases and
by side and the organ has great reserves. So that even disorder, wherever possible in animals models. In
if seven parts out of the eight of the liver is damaged present study, evaluation of hepatoprotective
even then the liver is in a position to carry on the preparation mentioned in Ayurveda is attempted on
normal physiological functions without any external modern lines to test its claimed effects in liver disorders.
clinical signs detectable by the physician.2 Plan of Work:
Universal Journal of Pharmacy, 06(02), March-April 2017 8
, Hepatocellular disease is known to be associated with C) Biochemical Parameters:
impaired hepatic drug metabolising capacity and 1. Serum glutamate oxaloacetate Transaminase (SGOT)
impaired activity of hepatic enzymes. Liver diseases are 2. Serum glutamate pyruvate Transaminase (SGPT)
of different aetiology and till do day the best remedy is 3. Alkaline Phosphatase.
from natural drugs only. Ayurvedic books have prescribed 4. Serum bilirubin
as number of formulations in the treatment of liver 5. Cholesterol
diseases. 6. Total Proteins
A number of indigenous formulations have been claimed 7. Albumin
to possess hepatoprotective activity. A few of the have 8. Globulin.
also been studied in experimental models. It will be of D) Histological changes Such as:
great value if all such formulations are biologically 1. Fatty degeneration 2.
Table 1: Active ingredient with their quantity of both formulations
Each Sugar coated Duliv Syrup
Common name Biological Name tablet contains Each ml contains.
(Formulation A) (Formulation B)
Sharpunkha TephrosiaPurpurea 150mg 200 mg
BhuiAvla Phyllanthusnuriri 40mg 50 mg
Maka Eclipta alba 40mg 50 mg
Sunth ZingiberOfficinale 10mg 25 mg
Tulas Ocimum Sanctum 10mg 25 mg
Hirda TerminaliaChebula 30mg 50 mg
Gulvel Tenosporacordifolia 20 mg 25 mg
Kalmegh AndrographisPeniculata 50 mg 20 mg
Punarnava Boerhaaviadiffussa 50 mg 100 mg
assessed on animal models, wherever possible, and also Focci of Necrosis
by clinical trials. This will be revealing the utility of 3. Congestions.
these formulations in liver diseases of varying aetiology. E) The statistical Significance.
“DULIV” Is an indigenous herbal formulation which is The overall objectives of these methods to be applied is
claimed to be hepatoprotective. It contains nine to prepared a monograph for the above mentioned
indigenous herbs which have been mentioned in hepatoprotective formulation.
Ayurveda as hepatoprotective.
In the present investigation effect of “DULIV TABLET” MATERIALS AND METHODS
and “DULIV SYRUP”prepared by incorporating well “DULIV”an indigenous herbal formulation contains nine
known indigenous plants was studied in Carbon indigenous herbs which have been mentioned in
tetrachloride induced liver damage in Albino Rats. The Ayurveda as hepatoprotective. However no scientific
entire experimentation was carried out in the following study of the formulation prepared from these plants has
way: been reported.
1. Procurement of drugs of the above mentioned The present study has been undertaken to evaluate the
formulation. role of DULIV as a function of time on the toxic effect
2. Determination of safe dose of these formulations by induced by CCL4challenge on Serum enzyme. DULIV was
using Albino mice. obtained as: Formulation ‘A’( Duliv Tablet)
3. Induction of hepatotoxicity in albino rats by using Formulation ‘B’( Duliv Surup)
CCL4. Tablet was crushed and the powder (100mg/kg p.o. daily
4. Treatment of toxicity by administering formulation for 15days) was given as freshly prepared suspension in
separately in different groups of albino rats. gum acacia. Control animals received only the vehicle.
5. Assessment of hepatoprotective effect by using the The literature survey has revealed that the
parameters as given below and subsequent entireingredients in this formulation are individually
comparison. having hepatoprotective activity. Determination of Safe
A) Functional Paramter. Dose (Toxicity studies): Duliv preparation was studied
1. Measurment of pentobarbitone sleeping time. B) for its hepatoprotective activity. Prior to determine this
Morphological Paramenters. effect the safe dose of the formulation A and
1. Weight of Liver. Formulation B was found out.
2. Volume of Liver.
Universal Journal of Pharmacy, 06(02), March-April 2017 9
