Central Dogma
DNA Replication 3 Possible replication patterns:
1. Semi-conservative replication
2. Conservative replication
3. Dispersive replication
Purpose:
cells need to make a copy of DNA before
dividing so each daughter cell has a
complete copy of genetic information
DNA Replication
Replication
Each parent strand serves as a template
DNA copies itself exactly
for a new strand and two new DNA
Occurs in the nucleus
strands each have one old and one new
natural replication strand
Polymerase chain reaction with the
use of thermocycler
artificial replication
Any mistake in copying leads to
mutation
DNA mutation
chromosomal mutation
, Replication Characteristics
Semi-conservative
bidirectional replication
Semi continuous replication
High fidelity
Meselson and Stahl Experiment(1958)
Semi-Conservative Replication
Half of the parental DNA molecule is
conserved in each new double helix,
paired with a newly synthesized
complementary strand
Replication of DNA base pairing
allows each strand to serve as a
template for a new strand
new strand is 1/2 parent
template &
1/2 new DNA
DNA Replication 3 Possible replication patterns:
1. Semi-conservative replication
2. Conservative replication
3. Dispersive replication
Purpose:
cells need to make a copy of DNA before
dividing so each daughter cell has a
complete copy of genetic information
DNA Replication
Replication
Each parent strand serves as a template
DNA copies itself exactly
for a new strand and two new DNA
Occurs in the nucleus
strands each have one old and one new
natural replication strand
Polymerase chain reaction with the
use of thermocycler
artificial replication
Any mistake in copying leads to
mutation
DNA mutation
chromosomal mutation
, Replication Characteristics
Semi-conservative
bidirectional replication
Semi continuous replication
High fidelity
Meselson and Stahl Experiment(1958)
Semi-Conservative Replication
Half of the parental DNA molecule is
conserved in each new double helix,
paired with a newly synthesized
complementary strand
Replication of DNA base pairing
allows each strand to serve as a
template for a new strand
new strand is 1/2 parent
template &
1/2 new DNA
