Summary Krebs Cycle

Conversion of Pyruvate to AcetyI-CoA
* Anytime CO2 is released it is said Scarbon pyruvate brought inside of
,
Mitochondria in the presence of O2 . Aerobic conditions on

to be that oxidative decarboxylation occurs.




·
P D H
. .
inhibits
Kinase

(inhibits P D . . H complex


Inhibitors a
Pyruvate & App
. P D
. . H Removes a 'p'd stimulates the
Phosphatase P D H
.
.
complex.

Alo
. Stimulators : Cast
Hormonal stimulator : insulin
So , P D H . .
is
regulated by reversible in adipose tissue .




Phosphorylation by P D H kinase
.
.
Phosphatase .




Regulation of P.D.H
i te m e n




P D H is
large multi-enzyme complex of :
-
.
. a . It consists


En Pyruvate dehydrogenase Es dihydrolipoyl transacetylase Es dihydrolipoyl dehydrogenase.
* *
·




Here ,

~ThyminepophosphateTreats
the hydrites
thisenzmewehave potewt
~
FAD picks up

from both SHd becomes FAD

it with only 2 carbons Acetyl group .
Carries the Acetyl group from El Done Then NAD" takes the hydrites from FADH2
attached to TPP .




& become NADH.
*
Sulfur attaches tothe acetyl , the other

~ This is called oxidative carb becomes SH
,
s
lipoate .
CoA comes
~
NADH then drops of the hydrites to
-a




oxylation of pyruvate
. in
& reacts with Acetyl & becomes Acetyl-CoA the electron transport chain
.




~
Inhibited by ATP & NADH . ~Now , the second S also becomes SH,
SH

Lipoate SH




·
Tpp , lipoate & FAD are prosthetic groups . NADT & CoA & SH are co-substrates

·
In En ovidative decarboxylation .
occurs In Ec Transfer of Acetyl to cot & oxidation occurs
. In Es
, , group

Regeneration of the oxidized form of lipoate occurs
.




Arsenite inhibits the P D H complex.
·
.
.