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Biology practical exam 2024/2025

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High educative biology practical exam with questions and answers

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A pretest and post test 2024-2025 actual practical biology exam with complete questions
and answers (verified answers). Already graded A+

1.

(a)

Specimen Volume of foam
Crushed cube A More than 5 ml of form (depending on the concentration);

(b) Cellular respiration produces toxic hydrogen peroxide; enzyme catalase breaks down toxic
hydrogen peroxide to harmless products; water and oxygen gas;

(c)

FOOD PROCEDURE OBSERVATION CONCLUSION
SUBSTANCE
Starch To 2ml of solution B in a Presence of starch;
test tube add 2 drops of Blue black; (Award mark if all
iodine solution; acc food Acc blue-black steps are correct)
substance for solution B
Reducing To 2ml of solution B, add Turns from blue Presence of reducing
sugars equal amount of to green to sugars;
Benedict’s solution and yellow to orange (Award mark if all
boil mixture in water to brown; steps are correct)
bath; acc food Acc brown
substance for solution B colour observed
No mark is awarded if the procedure is wrong

TEST PROCEDURE OBSERVATION CONCLUSION
TUBE
F To 3ml of food substance, add Turns from blue to Presence of
equal amount of Benedict’s green to yellow to reducing sugars;
solution and boil mixture in water orange to brown;
bath;
G To 2ml of food substance, add Blue colour of Absence of
equal amount of Benedict’s Benedict’s solution reducing sugars;
solution and boil mixture in water is retained;
bath;
H To 2ml of food substance, add Turns from blue to Presence of
equal amount of Benedict’s green to yellow to reducing sugars;
solution and boil mixture in water orange to brown;
bath;
K To 2ml of food substance, add Blue colour of Absence of
equal amount of Benedict’s Benedict’s solution reducing sugars;
solution and boil mixture in water is retained.
bath;
 One mark is awarded at the conclusion stage and only scored if the procedure and
observation are correct.
 No mark is awarded if the procedure is wrong.

Account for the observation in:
(i) Absence of reducing sugars, enzyme is denatured by hydrochloric acid; starch not broken
down to reducing sugars;

(ii) Presence of reducing sugars, enzyme zworks best in alkaline condition; starch is broken

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