Test Bank: Concepts of Genetics, 3rd Edition by Brooker ,
Chapters 1-24, 9781259879906 | Rationals Included
Which of the following is not required for accurate transcription?
A) Regulatory information within the DNA.
B) Proteins which bind to the DNA.
C) Proteins which copy the information from the DNA.
D) All of these are required.
E) None of these are required. - ANSWER:All of these are required
The closed promoter complex consists of all of the following except:
A) Promoter region of the DNA.
B) RNA polymerase.
C) Transcription factors.
D) Helicase. - ANSWER:Helicase
Which type(s) of RNA molecule has(have) the primary function of modifying other RNA molecules?
A) RNA of RNaseP.
B) snRNa.
C) scRNa.
D) A and B.
E) All of the above. - ANSWER:A and B
If you have a strain of bacteria that produce nonfunctional sigma factor, which step of transcription
would you expect will be affected?
A) These bacteria will be unable to assemble RNA polymerase onto the DNA strand.
B) These bacteria will be unable to identify and tightly bind promoter elements.
C) These bacteria will be unable to convert a closed complex to an open complex.
D) These bacteria will be unable to synthesize RNA once an open complex is formed. - ANSWER:These
bacteria will be unable to identify and tightly bind promoter elements.
Which of the following mutations would you expect to have the most significant effect on DNA
binding by the transcription factor depicted in figure 12.6?
A) A conservative substitution in alpha helix 1.
B) A conservative substitution in alpha helix 2.
C) A nonconservative substitution in alpha helix 3.
D) A nonconservative substitution in the turn region. - ANSWER:A nonconservative substitution in
alpha helix 3.
What is the primary factor involved in termination of RNA synthesis?
A) Temporary stabilization of the RNA:DNA hybrid region.
B) Synthesis of a specific termination signal in the RNA.
C) Destabilization of hydrogen bonding between DNA and RNA within the open complex.
D) Recognition of a termination signal on the DNA by termination proteins. - ANSWER:Destabilization
of hydrogen bonding between DNA and RNA within the open complex.
Which type of transcriptional termination requires formation of a stem loop secondary structure in
the RNA?
A) ρ dependent
B) ρ independent
C) polymerase independent
D) Both A and B
E) None of the above. - ANSWER:Both A and B
Intrinsic terminators are:
, A) RNA sequences that assist in termination of transcription.
B) DNA sequences that assist in termination of transcription.
C) Proteins that assist in termination of transcription.
D) All of these.
E) None of these. - ANSWER:RNA sequences that assist in termination of transcription.
Which statement could NOT describe both eukaryotic and prokaryotic transcription?
A) Promoter elements in the DNA are required for polymerase binding.
B) Transcription factors control the binding of RNA polymerase.
C) RNA polymerase opens a double stranded DNA to expose the template strand.
D) RNA polymerase catalyzes RNA synthesis from 3' to 5' of the new strand.
E) None of these (all apply to both eukaryotic and prokaryotic transcription). - ANSWER:RNA
polymerase catalyzes RNA synthesis from 3' to 5' of the new strand.
What would you expect to be the effect of a mutation of RNA polymerase that removed the rudder
region?
A) DNA would not be opened to expose the template strand.
B) Nucleotides would not base pair correctly with the template strand.
C) Nucleotides would not be connected into an RNA strand.
D) RNA would not be released from the template DNA. - ANSWER:RNA would not be released from
the template DNA.
Which of the following is an example of a cis-acting element in eukaryotic transcription?
A) RNA polymerase II.
B) Transcription factors.
C) Enhancers
D) All of these.
E) None of these. - ANSWER:Enhancers
Which of the following RNA modifications could best be described as "removal of intervening
sequences"?
A) Trimming.
B) 5' capping.
C) Poly-A tailing.
D) Splicing.
E) RNA editing or base modification. - ANSWER:Splicing
When studying eukaryotic enzymes in relation to their prokaryotic counterparts, you discover that
some eukaryotic enzymes seem to consist of several regions, each of which performs the function of a
separate enzyme in bacteria. Which of the following explanations would you be most likely to accept?
A) Random mutation has generated these multi-function eukaryotic enzymes.
B) A polycistronic transcript in bacteria became a polycistronic transcript in eukaryotic cells.
C) Several genes located near each other in bacteria became transcribed together and the
intervening sequences spliced out.
D) Similar regulatory elements for each of the prokaryotic proteins allowed them to be expressed
together. - ANSWER:Several genes located near each other in bacteria became transcribed together
and the intervening sequences spliced out.
Which of the following would be expected to demonstrate that mRNA contains introns?
A) A comparison of the DNA and mRNA sequences.
B) A comparison of the genomic DNA and cDNA sequences.
C) A hybridization between DNA and mRNA molecules.
D) All of these.
E) None of these. - ANSWER:All of these
Which of the following statements about RNA splicing is NOT correct?
A) Some introns can splice themselves out.
Chapters 1-24, 9781259879906 | Rationals Included
Which of the following is not required for accurate transcription?
A) Regulatory information within the DNA.
B) Proteins which bind to the DNA.
C) Proteins which copy the information from the DNA.
D) All of these are required.
E) None of these are required. - ANSWER:All of these are required
The closed promoter complex consists of all of the following except:
A) Promoter region of the DNA.
B) RNA polymerase.
C) Transcription factors.
D) Helicase. - ANSWER:Helicase
Which type(s) of RNA molecule has(have) the primary function of modifying other RNA molecules?
A) RNA of RNaseP.
B) snRNa.
C) scRNa.
D) A and B.
E) All of the above. - ANSWER:A and B
If you have a strain of bacteria that produce nonfunctional sigma factor, which step of transcription
would you expect will be affected?
A) These bacteria will be unable to assemble RNA polymerase onto the DNA strand.
B) These bacteria will be unable to identify and tightly bind promoter elements.
C) These bacteria will be unable to convert a closed complex to an open complex.
D) These bacteria will be unable to synthesize RNA once an open complex is formed. - ANSWER:These
bacteria will be unable to identify and tightly bind promoter elements.
Which of the following mutations would you expect to have the most significant effect on DNA
binding by the transcription factor depicted in figure 12.6?
A) A conservative substitution in alpha helix 1.
B) A conservative substitution in alpha helix 2.
C) A nonconservative substitution in alpha helix 3.
D) A nonconservative substitution in the turn region. - ANSWER:A nonconservative substitution in
alpha helix 3.
What is the primary factor involved in termination of RNA synthesis?
A) Temporary stabilization of the RNA:DNA hybrid region.
B) Synthesis of a specific termination signal in the RNA.
C) Destabilization of hydrogen bonding between DNA and RNA within the open complex.
D) Recognition of a termination signal on the DNA by termination proteins. - ANSWER:Destabilization
of hydrogen bonding between DNA and RNA within the open complex.
Which type of transcriptional termination requires formation of a stem loop secondary structure in
the RNA?
A) ρ dependent
B) ρ independent
C) polymerase independent
D) Both A and B
E) None of the above. - ANSWER:Both A and B
Intrinsic terminators are:
, A) RNA sequences that assist in termination of transcription.
B) DNA sequences that assist in termination of transcription.
C) Proteins that assist in termination of transcription.
D) All of these.
E) None of these. - ANSWER:RNA sequences that assist in termination of transcription.
Which statement could NOT describe both eukaryotic and prokaryotic transcription?
A) Promoter elements in the DNA are required for polymerase binding.
B) Transcription factors control the binding of RNA polymerase.
C) RNA polymerase opens a double stranded DNA to expose the template strand.
D) RNA polymerase catalyzes RNA synthesis from 3' to 5' of the new strand.
E) None of these (all apply to both eukaryotic and prokaryotic transcription). - ANSWER:RNA
polymerase catalyzes RNA synthesis from 3' to 5' of the new strand.
What would you expect to be the effect of a mutation of RNA polymerase that removed the rudder
region?
A) DNA would not be opened to expose the template strand.
B) Nucleotides would not base pair correctly with the template strand.
C) Nucleotides would not be connected into an RNA strand.
D) RNA would not be released from the template DNA. - ANSWER:RNA would not be released from
the template DNA.
Which of the following is an example of a cis-acting element in eukaryotic transcription?
A) RNA polymerase II.
B) Transcription factors.
C) Enhancers
D) All of these.
E) None of these. - ANSWER:Enhancers
Which of the following RNA modifications could best be described as "removal of intervening
sequences"?
A) Trimming.
B) 5' capping.
C) Poly-A tailing.
D) Splicing.
E) RNA editing or base modification. - ANSWER:Splicing
When studying eukaryotic enzymes in relation to their prokaryotic counterparts, you discover that
some eukaryotic enzymes seem to consist of several regions, each of which performs the function of a
separate enzyme in bacteria. Which of the following explanations would you be most likely to accept?
A) Random mutation has generated these multi-function eukaryotic enzymes.
B) A polycistronic transcript in bacteria became a polycistronic transcript in eukaryotic cells.
C) Several genes located near each other in bacteria became transcribed together and the
intervening sequences spliced out.
D) Similar regulatory elements for each of the prokaryotic proteins allowed them to be expressed
together. - ANSWER:Several genes located near each other in bacteria became transcribed together
and the intervening sequences spliced out.
Which of the following would be expected to demonstrate that mRNA contains introns?
A) A comparison of the DNA and mRNA sequences.
B) A comparison of the genomic DNA and cDNA sequences.
C) A hybridization between DNA and mRNA molecules.
D) All of these.
E) None of these. - ANSWER:All of these
Which of the following statements about RNA splicing is NOT correct?
A) Some introns can splice themselves out.
