ASCP HT EXAM (2024-2025) QUESTIONS WITH
ANSWERS
Alcohols
Acetone
Acetic Acid*
Coagulates nuclear proteins but not the cytoplasm
Name Non-additive fixatives
Over stained hematoxylin
Decrease incubation in hematoxylin, increase differentiation,
or cut thinner sections.
What issue is seen in this H&E stain? How can you correct
this issue?
Nuclear bubbling
Inadequate fixation or dehydration, or drying slides in a hot
oven before air drying
What issue is seen in this H&E stain? How can you correct
this issue?
Reddish/brown nuclei
Over oxidized hematoxylin or insufficient bluing
, ASCP HT EXAM (2024-2025) QUESTIONS WITH
ANSWERS
What issue is seen in this H&E stain? How can you correct
this issue?
Over staining with eosin
Check the concentration of eosin, decrease time in eosin,
ensure correct timing in dehydrating solution, or checking
section thickness
What issue is seen in this H&E stain? How can you correct
this issue?
Uneven staining
Hematoxylin solution is too low
What issue is seen in this H&E stain? How can you correct
this issue?
Feulgen reaction
What stain is shown in the picture?
Tumor of mesenchymal origin
What is a Sarcoma?
, ASCP HT EXAM (2024-2025) QUESTIONS WITH
ANSWERS
Tumor of lymphoid tissue?
What is a Lymphoma?
malignant disease with large numbers of immature white
blood cells
What is Leukemia?
Caner that originates in the plasma cell of the bone marrow.
What is a myeloma?
Recognizes many epitopes from one antigen. The antigen is
injected into the host and then the host serum is selected.
Define polyclonal antibodies
One antibody serviced from a single clone B cell. The antigen
is injected into the host and the host is sacrificed.
Define monoclonal antibodies
, ASCP HT EXAM (2024-2025) QUESTIONS WITH
ANSWERS
B-cells
Hybridoma
Myeloma
What makes up the culture used for monoclonal antibodies?
A primary antibody that is labeled with a fluorochrome.
What is a direct IHC method?
A primary antibody with a secondary antibody labeled with a
fluorochrome
What is an indirect IHC method?
Primary antibody
What is the secondary antibody raised against?
A catalyst for biochemical reaction.
What is an enzyme?
ANSWERS
Alcohols
Acetone
Acetic Acid*
Coagulates nuclear proteins but not the cytoplasm
Name Non-additive fixatives
Over stained hematoxylin
Decrease incubation in hematoxylin, increase differentiation,
or cut thinner sections.
What issue is seen in this H&E stain? How can you correct
this issue?
Nuclear bubbling
Inadequate fixation or dehydration, or drying slides in a hot
oven before air drying
What issue is seen in this H&E stain? How can you correct
this issue?
Reddish/brown nuclei
Over oxidized hematoxylin or insufficient bluing
, ASCP HT EXAM (2024-2025) QUESTIONS WITH
ANSWERS
What issue is seen in this H&E stain? How can you correct
this issue?
Over staining with eosin
Check the concentration of eosin, decrease time in eosin,
ensure correct timing in dehydrating solution, or checking
section thickness
What issue is seen in this H&E stain? How can you correct
this issue?
Uneven staining
Hematoxylin solution is too low
What issue is seen in this H&E stain? How can you correct
this issue?
Feulgen reaction
What stain is shown in the picture?
Tumor of mesenchymal origin
What is a Sarcoma?
, ASCP HT EXAM (2024-2025) QUESTIONS WITH
ANSWERS
Tumor of lymphoid tissue?
What is a Lymphoma?
malignant disease with large numbers of immature white
blood cells
What is Leukemia?
Caner that originates in the plasma cell of the bone marrow.
What is a myeloma?
Recognizes many epitopes from one antigen. The antigen is
injected into the host and then the host serum is selected.
Define polyclonal antibodies
One antibody serviced from a single clone B cell. The antigen
is injected into the host and the host is sacrificed.
Define monoclonal antibodies
, ASCP HT EXAM (2024-2025) QUESTIONS WITH
ANSWERS
B-cells
Hybridoma
Myeloma
What makes up the culture used for monoclonal antibodies?
A primary antibody that is labeled with a fluorochrome.
What is a direct IHC method?
A primary antibody with a secondary antibody labeled with a
fluorochrome
What is an indirect IHC method?
Primary antibody
What is the secondary antibody raised against?
A catalyst for biochemical reaction.
What is an enzyme?
