BCH-305/BIOT-304/MIC-304
Nucleic acid chemistries
Dr. Pakeeza Arzoo Shaiq
Spring, 2024
Selected Reading:
1. Chapter 4
BIOCHEMISTRY by Berg, Tymoczko and Stryer (9 th ed.)
2. Chapter 8, Lehninger’s principles of Biochemistry
, Melting kinetics of NA
• Tm: (melting temperature)
temperature at which half
of the DNA is melted
• Denaturation is a
cooperative process;
– caused by: heat,
change in pH,
organic solvents
(urea, formamide)
Recall tautomeric shifts
08/23/2024 BCH-305 Dr. Pakeeza 2
,
,
Nucleic acid chemistries
Dr. Pakeeza Arzoo Shaiq
Spring, 2024
Selected Reading:
1. Chapter 4
BIOCHEMISTRY by Berg, Tymoczko and Stryer (9 th ed.)
2. Chapter 8, Lehninger’s principles of Biochemistry
, Melting kinetics of NA
• Tm: (melting temperature)
temperature at which half
of the DNA is melted
• Denaturation is a
cooperative process;
– caused by: heat,
change in pH,
organic solvents
(urea, formamide)
Recall tautomeric shifts
08/23/2024 BCH-305 Dr. Pakeeza 2
,
,
