100% Correct Questions And Answers
Containment - ANSWER-safe methods and procedures to handle micro organisms
Aseptic technique prevents contamination of - ANSWER-You
Others
Experiment
Work Area
External Enviroment
Ubiquitous - ANSWER-found everywhere
Pathogen - ANSWER-micro organisms that cause disease
Opportunistic pathogen - ANSWER-cause disease in right circumstances
Inoculation - ANSWER-implantation of micro organisms on or in media
Culture - ANSWER-cultivation of the micro organisms
What has actually grown
Turbid - ANSWER-cloudy inoculation of growth
Colony - ANSWER-A discrete mass of millions or more cells ideally from one cell
Points of first few labs - ANSWER-practice aseptic technique
Sterile - ANSWER-completely free of all life
Pure culture - ANSWER-one type of colony
Mixed culture - ANSWER-more than one type of colony
Parfocal - ANSWER-once its in focus it stays in focus as you move un in magnification
Parcentric - ANSWER-What is in the center will stay in the center
Bio safety levels (BSL) measure what? - ANSWER-an organisms controllability,
infectivity, virulence, pathogenicity, antibiotic resistance, vaccine, treatment, mortality.
BSL 1 - ANSWER-does not normally cause disease in people
, BSL 2 - ANSWER-Associated with disease
BSL 3 - ANSWER-indigenous/exotic agents that may have serious or lethal
consequences and with potential for aerosol transmission
BSL 4 - ANSWER-dangerous/exotic agents of a life-threatening nature or unknown risk
of transmission
Why is oil needed for high magnification - ANSWER-oil is needed to refract light the
same as glass does
4x - ANSWER-scanning
10x - ANSWER-low power
40x - ANSWER-high power (high dry)
100x - ANSWER-oil imersion
Total magnification - ANSWER-ocular magnification X objective magnification
Working distance - ANSWER-space between slide and lens
As magnification increases field of view.... - ANSWER-decreases
Resolution - ANSWER-clarity of an image produced by a set of lenses
Resolving power - ANSWER-ability of a lens to separate tow objects in a microscopic
field
Function of wavelength of light and a property of the lens called numerical aperture
Numerical aperture - ANSWER-mathematical expression that describes the ability of the
condenser lens to concentrate light rays from the light source
Some refracted light is always lost
What are the four factors for maximum resolution and adequate contrast - ANSWER-
Blue filter
Condenser position
Diaphram position
Immersion Oil
What does a three lens system consist of? - ANSWER-Occulars
Objectives
Condenser