Biol-2421 Exam 2 |Questions with 100%
Solutions/Verified Answers
Select all that apply
Microscopic examination is important in the presumptive identification of an organism
because it can provide information on the organism's ______.
size and shape
metabolic activity
protein profile
staining characteristics - ANSWER Size and shape, Staining Characteristics
Choose the correct descriptions of serological tests.
A)Require detection by antibodies.
B)Rely on differences of surface structure molecules such as components of the cell
wall, capsule, flagella, or pili.
C)Depend on bacteria of a given genus having the same surface structure molecules (cell wall,
capsule, flagella, or pili).
D)Must be conducted in the patient to obtain the response of the patient's immune system to
the particular bacterial strain being studied. - ANSWER Require detection by antibodies.
Rely on differences of surface structure molecules such as components of the cell wall, capsule,
flagella, or pili.
Select all that apply
MALDI-TOF mass spectrometry is an important new technology in bacterial
identification because it can identify an organism from a colony ______.
based on its DNA sequence
rapidly, often in less than 15 minutes
,based on the profile of its proteins - ANSWER rapidly, often in less than 15 minutes
based on the profile of its proteins
_________media are designed to reveal specific colony characteristics that can help in the
initial identification of bacteria in clinical samples. - ANSWER Differential
Which of the following is a major advantage of detecting specific nucleotide sequences
to identify prokaryotes? - ANSWER The organisms do not need to be grown in culture.
Which of the following accurately describe biochemical tests used to identify microorganisms?
They all look at the ability of bacteria to ferment various sugars.
They generally provide a more certain identification than just relying on colony morphology.
They rely on differences in metabolic capabilities of microorganisms.
They give results that are definitive for a specific microorganism.
They are always conducted quickly, with results found in just minutes. - ANSWER They
generally provide a more certain identification than just relying on colony morphology.
They rely on differences in metabolic capabilities of microorganisms.
Why are sequences of ribosomal RNA so useful in microbial classification and identification?
A.)Once a sequence is determined, it can be compared with sequences of known organisms
by searching extensive databases.
B.)They allow identification without culturing the organism.
C.)The sequences are very different, which makes it easier to distinguish between
different microbes.
D.)The sequences are relatively stable, since the ribosome would not function with too
many mutations.
F.)The 16S ribosomal RNA found in prokaryotes is easier to sequence than the 18S
ribosomal RNA found in eukaryotes. - ANSWER A.)Once a sequence is determined, it can be
compared with sequences of known organisms by searching extensive databases.
B.)They allow identification without culturing the organism.
, D.)The sequences are relatively stable since the ribosome would not function with too
many mutations.
More than 300 cases of salmonellosis occur within a large city. Each time, Salmonella enterica is
isolated from the feces of the patient. Based on that information, the news media report that
there must be a common source to all these cases. What additional information would be
needed to support or disprove this view? - ANSWER The specific strain of the Salmonella would
need to be identified.
________testing uses antibodies to detect characteristic proteins or polysaccharides that make
up microorganisms. - ANSWER Serological
A group of strains that have a characteristic biochemical pattern is called a _____, or biotype. -
ANSWER biovar
_______- is a revolutionary new technology being used in clinical labs that can rapidly identify
an organism based on its protein profile. - ANSWER MALDI-TOF mass spectrometry
The designation O157:H7 of E. coli is an example of which of the following?
Biochemical typing
Serological typing
Phage typing
Molecular typing
Antibiograms - ANSWER Serological
True or false: Some methods of detecting specific nucleotide sequences make it possible to
identify organisms that cannot yet be grown in culture. - ANSWER true
Pulse Net, Genome Trakr, and Global Microbial Identifier are examples of surveillance networks
and programs that use _____ _______ sequence (WGS) data to help track infectious disease
outbreaks both - ANSWER whole genome
Solutions/Verified Answers
Select all that apply
Microscopic examination is important in the presumptive identification of an organism
because it can provide information on the organism's ______.
size and shape
metabolic activity
protein profile
staining characteristics - ANSWER Size and shape, Staining Characteristics
Choose the correct descriptions of serological tests.
A)Require detection by antibodies.
B)Rely on differences of surface structure molecules such as components of the cell
wall, capsule, flagella, or pili.
C)Depend on bacteria of a given genus having the same surface structure molecules (cell wall,
capsule, flagella, or pili).
D)Must be conducted in the patient to obtain the response of the patient's immune system to
the particular bacterial strain being studied. - ANSWER Require detection by antibodies.
Rely on differences of surface structure molecules such as components of the cell wall, capsule,
flagella, or pili.
Select all that apply
MALDI-TOF mass spectrometry is an important new technology in bacterial
identification because it can identify an organism from a colony ______.
based on its DNA sequence
rapidly, often in less than 15 minutes
,based on the profile of its proteins - ANSWER rapidly, often in less than 15 minutes
based on the profile of its proteins
_________media are designed to reveal specific colony characteristics that can help in the
initial identification of bacteria in clinical samples. - ANSWER Differential
Which of the following is a major advantage of detecting specific nucleotide sequences
to identify prokaryotes? - ANSWER The organisms do not need to be grown in culture.
Which of the following accurately describe biochemical tests used to identify microorganisms?
They all look at the ability of bacteria to ferment various sugars.
They generally provide a more certain identification than just relying on colony morphology.
They rely on differences in metabolic capabilities of microorganisms.
They give results that are definitive for a specific microorganism.
They are always conducted quickly, with results found in just minutes. - ANSWER They
generally provide a more certain identification than just relying on colony morphology.
They rely on differences in metabolic capabilities of microorganisms.
Why are sequences of ribosomal RNA so useful in microbial classification and identification?
A.)Once a sequence is determined, it can be compared with sequences of known organisms
by searching extensive databases.
B.)They allow identification without culturing the organism.
C.)The sequences are very different, which makes it easier to distinguish between
different microbes.
D.)The sequences are relatively stable, since the ribosome would not function with too
many mutations.
F.)The 16S ribosomal RNA found in prokaryotes is easier to sequence than the 18S
ribosomal RNA found in eukaryotes. - ANSWER A.)Once a sequence is determined, it can be
compared with sequences of known organisms by searching extensive databases.
B.)They allow identification without culturing the organism.
, D.)The sequences are relatively stable since the ribosome would not function with too
many mutations.
More than 300 cases of salmonellosis occur within a large city. Each time, Salmonella enterica is
isolated from the feces of the patient. Based on that information, the news media report that
there must be a common source to all these cases. What additional information would be
needed to support or disprove this view? - ANSWER The specific strain of the Salmonella would
need to be identified.
________testing uses antibodies to detect characteristic proteins or polysaccharides that make
up microorganisms. - ANSWER Serological
A group of strains that have a characteristic biochemical pattern is called a _____, or biotype. -
ANSWER biovar
_______- is a revolutionary new technology being used in clinical labs that can rapidly identify
an organism based on its protein profile. - ANSWER MALDI-TOF mass spectrometry
The designation O157:H7 of E. coli is an example of which of the following?
Biochemical typing
Serological typing
Phage typing
Molecular typing
Antibiograms - ANSWER Serological
True or false: Some methods of detecting specific nucleotide sequences make it possible to
identify organisms that cannot yet be grown in culture. - ANSWER true
Pulse Net, Genome Trakr, and Global Microbial Identifier are examples of surveillance networks
and programs that use _____ _______ sequence (WGS) data to help track infectious disease
outbreaks both - ANSWER whole genome
