code i write
,
Any X
CH 2 :
BASIC COMPONENTS OF LIVING SYSTEMS means HANGE cabbreviated
MICROSCOPES learn HOW magnification
& Total
~ in Mm .
to calibrate , etc .
e =
eyepiece < objective
I
- image MAGNIFICATION
=
# of times greater that an image is than the actual object
A m
↓ ↓
actual RESOLUTION
magnification
=
ability of a microscope to show 2 objects as SEPARATe !!
I
scanningSE
Transmission
LIGHT CONFOCAL TEM
2D
To form image
uses la ser electrons
light
-
max .
magnific >
-
will be eyepiece
ation lens
X
-
X 1 , 000 ,
000 X 500 , 000
X
1500
max reso ,
higher than 0 5 hm
200nm
.
3 -
10nm
Cdist between light
two objects)
# nigrosin / congo
reach Fluorescent
Colour-crystal Violet
stains dyes , a methylene Dyes metals-lead
bine #D
differential #
staining-bianisms
↳ Hematoxalin
osin
nucleic acid
=
turns blue
=
Pink/red-proteins
living/dead living thick 3D
,
organelles thick
advantages
#
specimen
Ip1 /
low resolution dead-vacumm dead vacumm
disadvantages photodamage cells
,
large structures very thin
Slow Baw
--
cell bu
nucleus
, animal
Revise-what do each do ?
Secretory < normal
cell
ANIMAL
RIBOSOmes OSMOOTH
①microfilaments
actin !!
② microtubules
tubulin !I
also
CENTRIOLES CRNA) s ISS
-
no ribosome
contractile fibres protein syn LIPID& CARB
snape F ② ROUGH
- .
:
QG
-
- -
-
more / contract -
movement -
float freely/RER synthesis ,
CYTOKINESIS SPINDLE FIBRes -
made in nuc .
storage
-
ribosomes
-
protein transport
ER
Intermediate fibres
③ folded mem .
CYTOSKELETOR -
mechanical strength
PROTEIN ! ↳ hollow
tubes CISTERNAe
present in cytoplasm
PRODUCTION
-
-
Keep / Shape
made of PROTEIN ORGANS
!
-
!
GOLGI APPARATUS
-
stacked flattened
Cisterne SER
sortn package
CELL PLASMA
-
proteins
Q
membrane -
receive/produce
-
differentially vesicles
permeable
-
movement of
solutes
CytoPlasm MITOCHONDRIA
NUCLEAR CILIA
NUCLEOLUS
-
water
aerobic respiration FLAgELLA
ENVELOPE
-
-
solutes Whip mobile &
folded inner ↑SA
mi
-
cx2mem) - Stationary
↳
enclose membrane
& protect DNA CTRACHEC)
synexte .
↳
NUCLEUS
CDNA molecules
LORISTAC
protein Synthesis
=
lysosomes
>
-
pigment grana
CELL kill Body
I
chloroplasts
Gu STRUCTURE - contain enzymes
to break down
Photosyn .
food molecules ,
> thylakoids
-
double cell wasted
(e)
membrane O +
worn out
-
make owN proteins , = stroma
break down
DNA ribosomes
-
,
PATHOgenS PLANT
Clite mito)
CELL WALL
-
Cellulose
microfibrils
-
freely per
DHYSICAL protect
-
pathogen
VACUOLES
-
prevent HYPOTONIC -
membrane
-
turgor pressure lighed sacs
W SAP
-
permanent D
small & transient
*
in animal
= large/turgor
membrane - tonoplast
PLANT
,
Any X
CH 2 :
BASIC COMPONENTS OF LIVING SYSTEMS means HANGE cabbreviated
MICROSCOPES learn HOW magnification
& Total
~ in Mm .
to calibrate , etc .
e =
eyepiece < objective
I
- image MAGNIFICATION
=
# of times greater that an image is than the actual object
A m
↓ ↓
actual RESOLUTION
magnification
=
ability of a microscope to show 2 objects as SEPARATe !!
I
scanningSE
Transmission
LIGHT CONFOCAL TEM
2D
To form image
uses la ser electrons
light
-
max .
magnific >
-
will be eyepiece
ation lens
X
-
X 1 , 000 ,
000 X 500 , 000
X
1500
max reso ,
higher than 0 5 hm
200nm
.
3 -
10nm
Cdist between light
two objects)
# nigrosin / congo
reach Fluorescent
Colour-crystal Violet
stains dyes , a methylene Dyes metals-lead
bine #D
differential #
staining-bianisms
↳ Hematoxalin
osin
nucleic acid
=
turns blue
=
Pink/red-proteins
living/dead living thick 3D
,
organelles thick
advantages
#
specimen
Ip1 /
low resolution dead-vacumm dead vacumm
disadvantages photodamage cells
,
large structures very thin
Slow Baw
--
cell bu
nucleus
, animal
Revise-what do each do ?
Secretory < normal
cell
ANIMAL
RIBOSOmes OSMOOTH
①microfilaments
actin !!
② microtubules
tubulin !I
also
CENTRIOLES CRNA) s ISS
-
no ribosome
contractile fibres protein syn LIPID& CARB
snape F ② ROUGH
- .
:
QG
-
- -
-
more / contract -
movement -
float freely/RER synthesis ,
CYTOKINESIS SPINDLE FIBRes -
made in nuc .
storage
-
ribosomes
-
protein transport
ER
Intermediate fibres
③ folded mem .
CYTOSKELETOR -
mechanical strength
PROTEIN ! ↳ hollow
tubes CISTERNAe
present in cytoplasm
PRODUCTION
-
-
Keep / Shape
made of PROTEIN ORGANS
!
-
!
GOLGI APPARATUS
-
stacked flattened
Cisterne SER
sortn package
CELL PLASMA
-
proteins
Q
membrane -
receive/produce
-
differentially vesicles
permeable
-
movement of
solutes
CytoPlasm MITOCHONDRIA
NUCLEAR CILIA
NUCLEOLUS
-
water
aerobic respiration FLAgELLA
ENVELOPE
-
-
solutes Whip mobile &
folded inner ↑SA
mi
-
cx2mem) - Stationary
↳
enclose membrane
& protect DNA CTRACHEC)
synexte .
↳
NUCLEUS
CDNA molecules
LORISTAC
protein Synthesis
=
lysosomes
>
-
pigment grana
CELL kill Body
I
chloroplasts
Gu STRUCTURE - contain enzymes
to break down
Photosyn .
food molecules ,
> thylakoids
-
double cell wasted
(e)
membrane O +
worn out
-
make owN proteins , = stroma
break down
DNA ribosomes
-
,
PATHOgenS PLANT
Clite mito)
CELL WALL
-
Cellulose
microfibrils
-
freely per
DHYSICAL protect
-
pathogen
VACUOLES
-
prevent HYPOTONIC -
membrane
-
turgor pressure lighed sacs
W SAP
-
permanent D
small & transient
*
in animal
= large/turgor
membrane - tonoplast
PLANT
