Culture Media
History
Louis Pasteur used urine & meat broth.
Had many disadvantages
Difficult to isolate bacteria from mixed
population
Earliest solid medium, cooked cut potato
used by Robert Koch.
Agar obtained from sea weeds universally
now.
Chief constituents of agar agar
Main constituent of agar is long chain
polysaccharides also inorganic salts &
protein like substance.
Unique property is is melts 98 c and sets at
42 c.
2% employed for solid media.
Types of media
Classification
1) Solid media
Liquid media
Semisolid media
2) Simple media
Complex media
Synthetic media
Special media
, Types of media
Special media
a.Enriched media
b.Enrichment media
c.Selective media
d.Indicator media
e.Differential media
f.Sugar media
g.Transport media
3) Aerobic media
4) Anaerobic media
Simple media.(Basal media)
Nutrient Agar
History
Louis Pasteur used urine & meat broth.
Had many disadvantages
Difficult to isolate bacteria from mixed
population
Earliest solid medium, cooked cut potato
used by Robert Koch.
Agar obtained from sea weeds universally
now.
Chief constituents of agar agar
Main constituent of agar is long chain
polysaccharides also inorganic salts &
protein like substance.
Unique property is is melts 98 c and sets at
42 c.
2% employed for solid media.
Types of media
Classification
1) Solid media
Liquid media
Semisolid media
2) Simple media
Complex media
Synthetic media
Special media
, Types of media
Special media
a.Enriched media
b.Enrichment media
c.Selective media
d.Indicator media
e.Differential media
f.Sugar media
g.Transport media
3) Aerobic media
4) Anaerobic media
Simple media.(Basal media)
Nutrient Agar
