Basic Safety Rules of Microbiology Laboratory
• Know locations of laboratory safety showers, eyewash stations, and fire extinguishers. The
safety equipment may be located in the hallway near the laboratory entrance. • Know
emergency exit routes.
• Avoid skin and eye contact with chemicals
• Minimize all chemical exposures.
• No horseplay will be tolerated.
• Assume that all chemicals of unknown toxicity are highly toxic.
• Postwarning signs when unusual hazards, hazardous materials, hazardous equipment, or
other special conditions are present.
• Avoid distracting or startling persons working in the laboratory.
• Use equipment only for its designated purpose
• Combine reagents in their appropriate order, such as adding acid to water.
• Avoid adding solids to hot liquids.
• Alllaboratory personnel should place emphasis on safety and chemical hygiene at all
times. • Never leave containers of chemicals open.
• Allcontainers must have appropriate labels. Unlabeled chemicals should never be used.
• Do not taste or intentionally sniff chemicals.
• Never consume and/or store food or beverages or apply cosmetics in areas where hazardous
chemicals are used or stored.
• Do not use mouth suction for pipetting or starting a siphon.
• Wash exposed areas of the skin prior to leaving the laboratory.
• Long hair and lose clothing must be pulled back and secured from entanglement or
potential capture.
• No contact lenses should be worn around hazardous chemicals even when wearing safety
glasses.
• Laboratory safety glasses or goggles should be worn in any area where chemicals are used
or stored. They should also be worn any time there is a chance of splashes or particulates
to enter the eye.
• Closed-toe shoes must be worn at all times in the laboratory. Perforated shoes or sandals
are not appropriate.
• Determine the potential hazards and appropriate safety precautions before beginning any
work.
• Procedures should be developed that minimize the formation and dispersion of aerosols. • If
• Know locations of laboratory safety showers, eyewash stations, and fire extinguishers. The
safety equipment may be located in the hallway near the laboratory entrance. • Know
emergency exit routes.
• Avoid skin and eye contact with chemicals
• Minimize all chemical exposures.
• No horseplay will be tolerated.
• Assume that all chemicals of unknown toxicity are highly toxic.
• Postwarning signs when unusual hazards, hazardous materials, hazardous equipment, or
other special conditions are present.
• Avoid distracting or startling persons working in the laboratory.
• Use equipment only for its designated purpose
• Combine reagents in their appropriate order, such as adding acid to water.
• Avoid adding solids to hot liquids.
• Alllaboratory personnel should place emphasis on safety and chemical hygiene at all
times. • Never leave containers of chemicals open.
• Allcontainers must have appropriate labels. Unlabeled chemicals should never be used.
• Do not taste or intentionally sniff chemicals.
• Never consume and/or store food or beverages or apply cosmetics in areas where hazardous
chemicals are used or stored.
• Do not use mouth suction for pipetting or starting a siphon.
• Wash exposed areas of the skin prior to leaving the laboratory.
• Long hair and lose clothing must be pulled back and secured from entanglement or
potential capture.
• No contact lenses should be worn around hazardous chemicals even when wearing safety
glasses.
• Laboratory safety glasses or goggles should be worn in any area where chemicals are used
or stored. They should also be worn any time there is a chance of splashes or particulates
to enter the eye.
• Closed-toe shoes must be worn at all times in the laboratory. Perforated shoes or sandals
are not appropriate.
• Determine the potential hazards and appropriate safety precautions before beginning any
work.
• Procedures should be developed that minimize the formation and dispersion of aerosols. • If
