DNA
Sequencing
Submitted
by
Monica. P
PALB 5272
, OUTLINE:
• INTRODUCTION
• TIMELINE
• CLASSIFICATION
• PRINCIPLES INVOLVED IN:
FIRST GENERATION SEQUENCING
SECOND GENERATION SEQUENCING
THIRD GENERATION SEQUENCING
• CONCLUSION
Dept of Plant Biotechnology
, Introduction
Determination of precise order of nucleotides
within a DNA molecule.
Make things bigger, better, faster, cheaper:
• less input DNA, simpler library preparation, less
reagents (no “washing”)
• smaller, less expensive machines
• reduced run time
• longer reads
• better quality
• higher throughput
Dept of Plant Biotechnology
, What generation are
we in?!
Second Generation Third Generation
First Generation
Platforms Platforms
Maxam Gilbert’s 454 / Roche Helicos
method (Pyrosequencing) (Reversible
(Chemical Terminators)
degradation Illumina (Reversible
method) Terminators)
PacBio (SMRT)
Applied Biosystems
Sanger’s
(SOLiD)
method (Chain
Oxford Nanopore
termination Ion Torrent (Nanopore
method) (PGM) sequencing)
Dept of Plant Biotechnology
Sequencing
Submitted
by
Monica. P
PALB 5272
, OUTLINE:
• INTRODUCTION
• TIMELINE
• CLASSIFICATION
• PRINCIPLES INVOLVED IN:
FIRST GENERATION SEQUENCING
SECOND GENERATION SEQUENCING
THIRD GENERATION SEQUENCING
• CONCLUSION
Dept of Plant Biotechnology
, Introduction
Determination of precise order of nucleotides
within a DNA molecule.
Make things bigger, better, faster, cheaper:
• less input DNA, simpler library preparation, less
reagents (no “washing”)
• smaller, less expensive machines
• reduced run time
• longer reads
• better quality
• higher throughput
Dept of Plant Biotechnology
, What generation are
we in?!
Second Generation Third Generation
First Generation
Platforms Platforms
Maxam Gilbert’s 454 / Roche Helicos
method (Pyrosequencing) (Reversible
(Chemical Terminators)
degradation Illumina (Reversible
method) Terminators)
PacBio (SMRT)
Applied Biosystems
Sanger’s
(SOLiD)
method (Chain
Oxford Nanopore
termination Ion Torrent (Nanopore
method) (PGM) sequencing)
Dept of Plant Biotechnology
