Why is the aseptic technique important during laboratory activities like smear
preparation?
- Aseptic technique helps prevent accidental infection of the handler
- proper aseptic technique aids in keeping microbes from spreading to other surfaces,
where they might be contacted by others in the lab
- the proper aseptic technique helps prevent contamination from being introduced into
your sample during preparation
Creating a bacterial smear from solid media, such as an agar plate or slant, often
feels more tangible than creating a smear from liquid media. Which of the
following is appropriate when creating a smear in this fashion?
using the loop, you should disperse the bacteria sample material into water drop on the
slide
As you create your smear from a sample on solid media, it is important to use an
appropriate amount of material and evenly disperse the organisms onto the
surface of the slide. Which of the following can occur due to using excessive
material as you create a smear preparation?
- it will be difficult to apply the stain correctly to the slide for visualization
- it will be difficult to distinguish bacterial cell morphology and arrangements of cells on
the smear sample
- it will be difficult to remove excess dye stain in later steps of the smear procedure
This is how your smear preparation looks in the microscope immediately after
completion.
True or False:
This image, as it appears, is able to be interpreted for information about the
organism(s) on the slide.
false
This is how your smear preparation looks in the microscope after simple staining
with methylene blue.
True or False:
This image, as it appears, is able to be interpreted for information about the
organism(s) on the slide
true
Now that you have seen the difference in the smear preparation after simple
methylene blue staining, which of the following is correct regarding your smear
now?
- helps show the intact appearance and localization of the cells, compared with no
staining
-helps visualize the shape and arrangements of bacterial cells that were difficult without
stain
Put the following steps in the correct order for performing a smear preparation
- appropriately label slide
- apply bacteria to the slide using aseptic technique
, - air drying
- heat fixation
- apply staining method
Based on what you learned, for which of the following samples would you skip
the heat fixation step?
- sample of pond water, looking for living bacteria activity such as observing for mobility
tools used in Gram staining
- appropriately label slide
- apply bacteria to the slide using aseptic technique
- air drying
- heat fixation
- apply staining method
sample of pond water, looking for living bacteria activity such as observing for
mobility
- This Image shows a cell arrangement of streptococci
- This image likely deserves a Gram stain bacterial smear
- This image shows a cell morphology of Cocci
After correct Gram staining, the gram-negative cells will appear ___ , whereas the
gram-positive cells appear ____.
red; purple
Your Gram stain is complete and correct. Which of the following statements
would apply to the image you see?Select all that apply
- You can observe gram-negative bacilli on this field.
- You can observe gram-positive cocci on this field.
You are Gram staining a known sample that contains BOTH gram- positive and
gram-negative bacteria. Are you complete the staining, you observe the following.
Which of the following statements would you apply to the image you see?
This image could be explained by the omission of the grams iodine step, causing all
cells to appear gram-negative
You are Gram staining a known sample that contains BOTH gram-positive and
gram-negative bacteria. Are you complete the staining, you realize that you did
not perform the safranin step. Which of the following statements would correctly
describe what you would see?
Graham negative organisms might not be visible
You are Gram staining a known sample that contains BOTH gram-positive and
gram-negative bacteria. After you complete the staining, you realize that you
performed the decolorizer step for 10 minutes instead of 10 seconds! Which of
the following statements would correctly describe what you would see?
Gram-positive organisms my incorrectly staying red
You are Gram staining a known sample that contains BOTH gram-positive and
gram-negative bacteria. After you complete the staining, you realize that you did
not use the alcohol bottle at all. Which cells would appear purple
gram-positive cells
preparation?
- Aseptic technique helps prevent accidental infection of the handler
- proper aseptic technique aids in keeping microbes from spreading to other surfaces,
where they might be contacted by others in the lab
- the proper aseptic technique helps prevent contamination from being introduced into
your sample during preparation
Creating a bacterial smear from solid media, such as an agar plate or slant, often
feels more tangible than creating a smear from liquid media. Which of the
following is appropriate when creating a smear in this fashion?
using the loop, you should disperse the bacteria sample material into water drop on the
slide
As you create your smear from a sample on solid media, it is important to use an
appropriate amount of material and evenly disperse the organisms onto the
surface of the slide. Which of the following can occur due to using excessive
material as you create a smear preparation?
- it will be difficult to apply the stain correctly to the slide for visualization
- it will be difficult to distinguish bacterial cell morphology and arrangements of cells on
the smear sample
- it will be difficult to remove excess dye stain in later steps of the smear procedure
This is how your smear preparation looks in the microscope immediately after
completion.
True or False:
This image, as it appears, is able to be interpreted for information about the
organism(s) on the slide.
false
This is how your smear preparation looks in the microscope after simple staining
with methylene blue.
True or False:
This image, as it appears, is able to be interpreted for information about the
organism(s) on the slide
true
Now that you have seen the difference in the smear preparation after simple
methylene blue staining, which of the following is correct regarding your smear
now?
- helps show the intact appearance and localization of the cells, compared with no
staining
-helps visualize the shape and arrangements of bacterial cells that were difficult without
stain
Put the following steps in the correct order for performing a smear preparation
- appropriately label slide
- apply bacteria to the slide using aseptic technique
, - air drying
- heat fixation
- apply staining method
Based on what you learned, for which of the following samples would you skip
the heat fixation step?
- sample of pond water, looking for living bacteria activity such as observing for mobility
tools used in Gram staining
- appropriately label slide
- apply bacteria to the slide using aseptic technique
- air drying
- heat fixation
- apply staining method
sample of pond water, looking for living bacteria activity such as observing for
mobility
- This Image shows a cell arrangement of streptococci
- This image likely deserves a Gram stain bacterial smear
- This image shows a cell morphology of Cocci
After correct Gram staining, the gram-negative cells will appear ___ , whereas the
gram-positive cells appear ____.
red; purple
Your Gram stain is complete and correct. Which of the following statements
would apply to the image you see?Select all that apply
- You can observe gram-negative bacilli on this field.
- You can observe gram-positive cocci on this field.
You are Gram staining a known sample that contains BOTH gram- positive and
gram-negative bacteria. Are you complete the staining, you observe the following.
Which of the following statements would you apply to the image you see?
This image could be explained by the omission of the grams iodine step, causing all
cells to appear gram-negative
You are Gram staining a known sample that contains BOTH gram-positive and
gram-negative bacteria. Are you complete the staining, you realize that you did
not perform the safranin step. Which of the following statements would correctly
describe what you would see?
Graham negative organisms might not be visible
You are Gram staining a known sample that contains BOTH gram-positive and
gram-negative bacteria. After you complete the staining, you realize that you
performed the decolorizer step for 10 minutes instead of 10 seconds! Which of
the following statements would correctly describe what you would see?
Gram-positive organisms my incorrectly staying red
You are Gram staining a known sample that contains BOTH gram-positive and
gram-negative bacteria. After you complete the staining, you realize that you did
not use the alcohol bottle at all. Which cells would appear purple
gram-positive cells
