MLT ASCP BOARD EXAM LATEST ACTUAL EXAM
ACD/CPD/CPD2 - ANSWER: 21 days
CPDA-1 - ANSWER: 35 days
Infants
HDFN from ABO - ANSWER: Spherocytes
1st pregnancy
O mother
Permanent deferral - ANSWER: Hepatitis after 11
HIV
T. Cruzi
CJD
1 unit or PRBC - ANSWER: Raises hemoglobin 1g and hematocrit 3%
Leukoreduced RBCs - ANSWER: Less than 5 x 10^6 to prevent febrile non hemolytic
reactions
Cryoprecipitate - ANSWER: Factor 8
vWF
Fibrinogen
For DIC
6 hours
Platelets 1unit - ANSWER: 5000 10000
Irradiated blood - ANSWER: Prevent GVHD
Ulex europaeus - ANSWER: Anti H lectin
Dolichos biflorus - ANSWER: Anti A1 lectin
Amount of H greatest to least - ANSWER: O
A2
B
A1
A1B
Most immunogenicity antigen - ANSWER: D
Weak D - ANSWER: Negative immediate spin and positive AHG
Weak D donor - ANSWER: Must be labeled Rh positive
,Weak D patient - ANSWER: Receives D negative
D control - ANSWER: AB positive
Any D negative
IgM antibodies - ANSWER: I
H
MN
P1
Lewis
IgG antibodies - ANSWER: Rh group
K
Duffy
Jk
I, i antibody - ANSWER: i converts to I as infant matures
Destroyed by enzymes - ANSWER: MN
S
Duffy
Enhanced by enzymes - ANSWER: Rh
Kids
Lewis
I
P1
Dosage - ANSWER: MNS
Rh
Kids
Duffy
Top three acute/immediate transfusion reactions and mortality - ANSWER: TRALI,
hemolytic transfusion reactions and TACO
IgE antibodies - ANSWER: Mild Allergic reactions
IgA antibodies - ANSWER: Severe transfusion allergic reaction
Anaphylactic
Positive hemolysis with negative DAT - ANSWER: Sickle cell crisis
Thalassemia/G6PD deficient
Unit overheated or frozen
All cells hemolysis
,Kernicterus - ANSWER: Excess bilirubin in newborn
HDFN from Rh - ANSWER: Retics
DAT positive
Immediate jaundice
After first baby
Rosette - ANSWER: Screening for fetal hemorrhage
Kleihauer Betke - ANSWER: Quantitative fetal maternal bleed
Primary immune response - ANSWER: IgM
Secondary immune response - ANSWER: IgG
Type 1 hypersensitivity - ANSWER: Anaphylactic
Immediate
Type 2 hypersensitivity - ANSWER: Antibody depending cytotoxicity
Transfusion reactions
Hashimotos
Good pasture
Type 3 hypersensitivity - ANSWER: Immune complex
Rheumatoid arthritis
SLE
Type 4 hyper sensitivity - ANSWER: Delayed
Monocytes and lymphocytes
Ouchterlony - ANSWER: Antibodies added to pre-cut wells in center of agar plate and
patient Sera and standards are alternated in wells surrounding the center well
EIA/ELISA - ANSWER: Sandwich technique
HCG
Nephelometry - ANSWER: Insoluble complexes
Why is pass-through suspension scattered light absorbance is proportional to the
number of insoluble complexes compared to standards
Antibody concentration
Immunofluorescence direct - ANSWER: Add florescence labeled anti-body to patient
tissue wash and examined under fluorescent microscope
Immunofluorescence indirect - ANSWER: Add patient serum to reagent wash add
florescence label to anti-globulin wash and examined under microscope
, FPIA (Fluorescence Polarization Immunoassay) - ANSWER: Add reagent antibody and
fluorescent tact antigen to patient serum
Increase polarize light as a negative test decrease polarized light as a positive test
Sensitivity - ANSWER: TP/ TP + FN x 100
Specificity - ANSWER: TN / TN + FP x 100
Non lattice - ANSWER: More sensitive immunoassays nephelometry
Lattice - ANSWER: Less sensitive
C reactive protein - ANSWER: Acute phase protein
Inflammation
Syphilis - ANSWER: T palladium
FTA abs
TPI
Dark field microscopy
VDRL - ANSWER: Syphilis CSF screening but can be positive for malaria
RPR - ANSWER: Charcoal for syphilis
More sensitive but Les specific
infectious mononucleosis - ANSWER: EBV
Lymphocytes
B cells
Burkets disease?
Autoimmune diseases - ANSWER: SLE
Sjögren's syndrome
Scleroderma
RA
Recent acute hepatitis A infection - ANSWER: Anti HaV
Acute hep B infection - ANSWER: Highly infectious
HBsAg
HBeAg
Chronic hep B - ANSWER: Carrier
Anti HBc
Past infection immunity to hep B - ANSWER: Anti HBe
Anti HBc
Anti HBs
ACD/CPD/CPD2 - ANSWER: 21 days
CPDA-1 - ANSWER: 35 days
Infants
HDFN from ABO - ANSWER: Spherocytes
1st pregnancy
O mother
Permanent deferral - ANSWER: Hepatitis after 11
HIV
T. Cruzi
CJD
1 unit or PRBC - ANSWER: Raises hemoglobin 1g and hematocrit 3%
Leukoreduced RBCs - ANSWER: Less than 5 x 10^6 to prevent febrile non hemolytic
reactions
Cryoprecipitate - ANSWER: Factor 8
vWF
Fibrinogen
For DIC
6 hours
Platelets 1unit - ANSWER: 5000 10000
Irradiated blood - ANSWER: Prevent GVHD
Ulex europaeus - ANSWER: Anti H lectin
Dolichos biflorus - ANSWER: Anti A1 lectin
Amount of H greatest to least - ANSWER: O
A2
B
A1
A1B
Most immunogenicity antigen - ANSWER: D
Weak D - ANSWER: Negative immediate spin and positive AHG
Weak D donor - ANSWER: Must be labeled Rh positive
,Weak D patient - ANSWER: Receives D negative
D control - ANSWER: AB positive
Any D negative
IgM antibodies - ANSWER: I
H
MN
P1
Lewis
IgG antibodies - ANSWER: Rh group
K
Duffy
Jk
I, i antibody - ANSWER: i converts to I as infant matures
Destroyed by enzymes - ANSWER: MN
S
Duffy
Enhanced by enzymes - ANSWER: Rh
Kids
Lewis
I
P1
Dosage - ANSWER: MNS
Rh
Kids
Duffy
Top three acute/immediate transfusion reactions and mortality - ANSWER: TRALI,
hemolytic transfusion reactions and TACO
IgE antibodies - ANSWER: Mild Allergic reactions
IgA antibodies - ANSWER: Severe transfusion allergic reaction
Anaphylactic
Positive hemolysis with negative DAT - ANSWER: Sickle cell crisis
Thalassemia/G6PD deficient
Unit overheated or frozen
All cells hemolysis
,Kernicterus - ANSWER: Excess bilirubin in newborn
HDFN from Rh - ANSWER: Retics
DAT positive
Immediate jaundice
After first baby
Rosette - ANSWER: Screening for fetal hemorrhage
Kleihauer Betke - ANSWER: Quantitative fetal maternal bleed
Primary immune response - ANSWER: IgM
Secondary immune response - ANSWER: IgG
Type 1 hypersensitivity - ANSWER: Anaphylactic
Immediate
Type 2 hypersensitivity - ANSWER: Antibody depending cytotoxicity
Transfusion reactions
Hashimotos
Good pasture
Type 3 hypersensitivity - ANSWER: Immune complex
Rheumatoid arthritis
SLE
Type 4 hyper sensitivity - ANSWER: Delayed
Monocytes and lymphocytes
Ouchterlony - ANSWER: Antibodies added to pre-cut wells in center of agar plate and
patient Sera and standards are alternated in wells surrounding the center well
EIA/ELISA - ANSWER: Sandwich technique
HCG
Nephelometry - ANSWER: Insoluble complexes
Why is pass-through suspension scattered light absorbance is proportional to the
number of insoluble complexes compared to standards
Antibody concentration
Immunofluorescence direct - ANSWER: Add florescence labeled anti-body to patient
tissue wash and examined under fluorescent microscope
Immunofluorescence indirect - ANSWER: Add patient serum to reagent wash add
florescence label to anti-globulin wash and examined under microscope
, FPIA (Fluorescence Polarization Immunoassay) - ANSWER: Add reagent antibody and
fluorescent tact antigen to patient serum
Increase polarize light as a negative test decrease polarized light as a positive test
Sensitivity - ANSWER: TP/ TP + FN x 100
Specificity - ANSWER: TN / TN + FP x 100
Non lattice - ANSWER: More sensitive immunoassays nephelometry
Lattice - ANSWER: Less sensitive
C reactive protein - ANSWER: Acute phase protein
Inflammation
Syphilis - ANSWER: T palladium
FTA abs
TPI
Dark field microscopy
VDRL - ANSWER: Syphilis CSF screening but can be positive for malaria
RPR - ANSWER: Charcoal for syphilis
More sensitive but Les specific
infectious mononucleosis - ANSWER: EBV
Lymphocytes
B cells
Burkets disease?
Autoimmune diseases - ANSWER: SLE
Sjögren's syndrome
Scleroderma
RA
Recent acute hepatitis A infection - ANSWER: Anti HaV
Acute hep B infection - ANSWER: Highly infectious
HBsAg
HBeAg
Chronic hep B - ANSWER: Carrier
Anti HBc
Past infection immunity to hep B - ANSWER: Anti HBe
Anti HBc
Anti HBs
