Staph. Aureus Strept. Pyogenes Pneumococci Gon
Morphology Gram positive cocci arranged Gram positive cocci arranged in chains Gram positive cocci arranged Gram negativ
in grape like cluster in pairs (diplococci) s
Nutrient agar: golden yellow Blood agar: ꞵ-hemolysis Choc
Cultures Blood agar: α-hemolysis
Blood agar: ꞵ-hemolysis Blood culture for bacteremia Modified Th
Mannitol salt agar: yellow
Biochemical Catalase positive Catalase negative Quellung test Oxida
reactions Coagulase positive Bacitracin sensitive Utili
1- Coagulase 1- Adherence: M protein - protein F - Capsule → 90 Type - 1- pili
2- Clumping factor Lipoteichoic acid antiphagocytic 2- Outer membra
3- invasins: leukocidins – 2- Antiphagocytic: M protein – c5a (Opa and pori
Virulence staphylokinase – peptidase – hyaluronic acid capsule 3- Lipooligosacch
hyaluronidase 3- Enzymes: streptokinase (fibrinolysin) 4- IgA Protease
4- protein A - Streptodornase (DNAse) -
5-Hemolysins Hyaluronidase.
6- Exotoxins (enterotoxins – 4- Toxins: Hemolysins (O and S) –
TSST – exfoliative toxin) Exotoxins (A-B-C).
A- Pyogenic diseases A- Localized: 1- Gonorrhea
1- Localized (skin infection – 1- pharyngitis Pneumonia A- Male → discha
surgical site infection – wound 2- scarlet fever (Toxin A) Meningitis (urethritis)
infection) 3- Skin infections (impetigo – cellulitis – Otitis media B- Female → Dis
Diseases 2- pneumonia erysipelas). Sinusitis (cervicitis and inf
3- bacteremia. B- Invasive Bacteremia rectum)
B- Toxigenic diseases 1- puerperal fever Conjunctivitis 2- Disseminated
1- Food poisoning (1-6 h 2- endocarditis Endocarditis (arthritis – bacter
incubation period) 3- necrotizing fasciitis (Toxin B) Pericarditis Rash – DIC)
2- Toxic shock syndrome. 4- Toxic shock (Toxin A-B-C) 3- Ophthalmia ne
3- SSSS (exfoliation) C- Post-streptococal diseases: born to infected
1- Acute rheumatic fever 4- vulvovaginitis
2- Acute glomerulonephritis
1- Specimen 1- Specimen 1- specimen 1- Specimen: disc
Morphology Gram positive cocci arranged Gram positive cocci arranged in chains Gram positive cocci arranged Gram negativ
in grape like cluster in pairs (diplococci) s
Nutrient agar: golden yellow Blood agar: ꞵ-hemolysis Choc
Cultures Blood agar: α-hemolysis
Blood agar: ꞵ-hemolysis Blood culture for bacteremia Modified Th
Mannitol salt agar: yellow
Biochemical Catalase positive Catalase negative Quellung test Oxida
reactions Coagulase positive Bacitracin sensitive Utili
1- Coagulase 1- Adherence: M protein - protein F - Capsule → 90 Type - 1- pili
2- Clumping factor Lipoteichoic acid antiphagocytic 2- Outer membra
3- invasins: leukocidins – 2- Antiphagocytic: M protein – c5a (Opa and pori
Virulence staphylokinase – peptidase – hyaluronic acid capsule 3- Lipooligosacch
hyaluronidase 3- Enzymes: streptokinase (fibrinolysin) 4- IgA Protease
4- protein A - Streptodornase (DNAse) -
5-Hemolysins Hyaluronidase.
6- Exotoxins (enterotoxins – 4- Toxins: Hemolysins (O and S) –
TSST – exfoliative toxin) Exotoxins (A-B-C).
A- Pyogenic diseases A- Localized: 1- Gonorrhea
1- Localized (skin infection – 1- pharyngitis Pneumonia A- Male → discha
surgical site infection – wound 2- scarlet fever (Toxin A) Meningitis (urethritis)
infection) 3- Skin infections (impetigo – cellulitis – Otitis media B- Female → Dis
Diseases 2- pneumonia erysipelas). Sinusitis (cervicitis and inf
3- bacteremia. B- Invasive Bacteremia rectum)
B- Toxigenic diseases 1- puerperal fever Conjunctivitis 2- Disseminated
1- Food poisoning (1-6 h 2- endocarditis Endocarditis (arthritis – bacter
incubation period) 3- necrotizing fasciitis (Toxin B) Pericarditis Rash – DIC)
2- Toxic shock syndrome. 4- Toxic shock (Toxin A-B-C) 3- Ophthalmia ne
3- SSSS (exfoliation) C- Post-streptococal diseases: born to infected
1- Acute rheumatic fever 4- vulvovaginitis
2- Acute glomerulonephritis
1- Specimen 1- Specimen 1- specimen 1- Specimen: disc
