Bacteriology
Bacteria Morphology Culture BRs Antib
1- 95% are
Nutrient agar (Yellow) Catalase positive
Staph aureus resistant →
Blood agar (ꞵ) Coagulase positive
2- MR
Gram positive MSA (Yellow)
Vanco
cocci arranged 3- VRSA →
Staph epidermidis in grape-like Blood agar (γ) Novobiocin sensitive Multi-drug
MSA (Non fermenter)
Staph
clusters Blood agar (γ) Novobiocin resistant Quino
saprophyticus
Blood agar (ꞵ) Catalase negative Peni
Strept. pyogenes Bacitracin sensitive Erythrom
Blood culture
Gram positive aller
Strept. agalactiae Blood agar (ꞵ) Bacitracin resistant Ampicilli
cocci arranged
deliv
Strept. viridans
in chains Blood agar (α) Optochin resistant Penicill
Blood culture Genta
Strept. Gram positive Ceftria
Blood agar (α) Optochin sensitive
pneumoniae Capsulated Vancom
Quellung test
diplococci Penic
Gram positive cocci Bile esculin agar Penic
Enterococci arranged in short (Black) aminogly
chains
Chocolate agar Ceftria
Neisseria Modified Thayer Oxidase positive Spectin
Gram negative Martin Utilize glucose Tetracyc
gonorrhea
kidney-shaped chlam
Capsulated
diplococci Ceftria
Neisseria Chocolate agar Oxidase positive Cefotaxime
Blood agar Utilize glucose + Maltose G
meningitides
Moraxella Gram negative Oxidase positive Multi-drug
catarrhalis diplococci
Loffler’s serum Club-ended / Chinese Antitoxi
C. diphtheria Blood tellurite letter appearance Penic
medium Elek’s test (for Toxin) Erythro
Listeria Gram positive Blood agar (ꞵ) Cold enrichment Ampi
monocytogenes Non spore
Gardnerella forming bacilli Whiff test Clue cells / PH >5
vaginalis
Lactobacilli Anaerobic Produce lactic acid
Propionibacterium Anaerobic produce propionic acid
acne
Nutrient agar Spore stain
Bacillus anthracis Ciprofl
Gram positive spore Blood agar (γ) Capsule stain
forming bacilli Two types (Emetic from Rice / Diarrheal from
Bacillus cereus (aerobic) meat dishes)
Bacteria Morphology Culture BRs Antib
1- 95% are
Nutrient agar (Yellow) Catalase positive
Staph aureus resistant →
Blood agar (ꞵ) Coagulase positive
2- MR
Gram positive MSA (Yellow)
Vanco
cocci arranged 3- VRSA →
Staph epidermidis in grape-like Blood agar (γ) Novobiocin sensitive Multi-drug
MSA (Non fermenter)
Staph
clusters Blood agar (γ) Novobiocin resistant Quino
saprophyticus
Blood agar (ꞵ) Catalase negative Peni
Strept. pyogenes Bacitracin sensitive Erythrom
Blood culture
Gram positive aller
Strept. agalactiae Blood agar (ꞵ) Bacitracin resistant Ampicilli
cocci arranged
deliv
Strept. viridans
in chains Blood agar (α) Optochin resistant Penicill
Blood culture Genta
Strept. Gram positive Ceftria
Blood agar (α) Optochin sensitive
pneumoniae Capsulated Vancom
Quellung test
diplococci Penic
Gram positive cocci Bile esculin agar Penic
Enterococci arranged in short (Black) aminogly
chains
Chocolate agar Ceftria
Neisseria Modified Thayer Oxidase positive Spectin
Gram negative Martin Utilize glucose Tetracyc
gonorrhea
kidney-shaped chlam
Capsulated
diplococci Ceftria
Neisseria Chocolate agar Oxidase positive Cefotaxime
Blood agar Utilize glucose + Maltose G
meningitides
Moraxella Gram negative Oxidase positive Multi-drug
catarrhalis diplococci
Loffler’s serum Club-ended / Chinese Antitoxi
C. diphtheria Blood tellurite letter appearance Penic
medium Elek’s test (for Toxin) Erythro
Listeria Gram positive Blood agar (ꞵ) Cold enrichment Ampi
monocytogenes Non spore
Gardnerella forming bacilli Whiff test Clue cells / PH >5
vaginalis
Lactobacilli Anaerobic Produce lactic acid
Propionibacterium Anaerobic produce propionic acid
acne
Nutrient agar Spore stain
Bacillus anthracis Ciprofl
Gram positive spore Blood agar (γ) Capsule stain
forming bacilli Two types (Emetic from Rice / Diarrheal from
Bacillus cereus (aerobic) meat dishes)
