Summary Practical Microbiology in Capsule

Practical Microbiology in Capsule

Culture Media
Medium Color Organism Growth
White / Yellow Simple medium
Nutrient agar Yellow Staph aureus Yellow endopigments
Green Pseudomonas aeruginosa Green exopigments
Swarming Proteus Swarming motility

Pink / yellow Staphylococci Mannitol fermentation
Mannitol salt agar Yellow Staph aureus Fermenter
Pink Staph epidermidis Non fermenter

Red Enriched medium Alpha / Beta / Gamma
Alpha  Green Pneumococci – Viridans Partial hemolysis
Blood agar streptococci
Beta  Yellow Strept pyogenes – Strept Complete hemolysis
(Clear zone) agalactiae – staph aureus
Gamma  White No hemolysis

Chocolate agar Brown Neisseria – Hemophilus Heated Blood
Loffler’s serum White (Tube) C. diphtheria Enriched medium
Lowenstein Jensen (LJ) Green M. tuberculosis Selective and enriched
Pink / yellow Enterobacteriaceae
MacConkey agar Pink E. coli / Klebsiella Lactose fermenters
Yellow Salmonella / shigella / proteus Lactose Non fermenters
Test sugar fermentation
Red Gram negative bacilli (Glucose-Lactose-Sucrose)
and H2S production.
Triple sugar iron (TSI) Red / Red P. aeruginosa No fermentation
Red / Yellow Salmonella / Shigella Ferment glucose
E. coli / Klebsiella  With GAS
Yellow / Yellow Cracking. Ferment all sugars
V. cholera  No GAS.
Red / Black Salmonella / Proteus Produce H2S
TCBS agar Green For vibrio Selective medium
Yellow V. cholerae Ferment sucrose
Cooked meat broth Meat inside a tube Anaerobic bacteria Anaerobic medium
Anaerobic Jar Anaerobic bacteria Anaerobic medium
(Gaspack)
Tissue culture bottles For Viruses Cultivation
SDA agar  Candida Creamy White Candida Selective for Fungi
Tetrathionate Yellow
Enrichment medium for salmonella
Selenite broth White