Difference between streak
plate and pour plate method
The plate-based method was first developed in the 1970s in Sweden and has been
integrated into public health nutrition tools of many countries, including the 2019
Canada's Food Guide and the United States'. A bacterial culture was spread on the glass
slide, then placed in the moist chamber with a small wet paper. Bacterial growth was
easily visible. Koch publicly demonstrated his plating method at the Seventh
International Medical Congress in London in August 1881. Since the development of the
agar plate in Robert Koch's laboratory, several methods have been used to achieve an
even distribution of bacterial growth on or in the agar. The most common methods used
to achieve this type of distribution are: streak, pour, thin-layer, spread and membrane
filter method.
WHAT IS STREAK PLATE METHOD?
The streak-plate procedure is designed to isolate pure cultures of bacteria, or colonies, from
mixed populations by simple mechanical separation. It involves spreading a small and
concentrated sample of microorganisms over the surface of a solid agar medium in a way that the
cells are progressively diluted and separated from each other. Single colonies are comprised of
millions of cells growing in a cluster on or within an agar plate. A colony, unlike a single cell, is
visible to the naked eye. It is commonly employed in environmental microbiology, clinical
microbiology, and research laboratories. Here is a step-by-step description of the streak plate
method:
, Inoculation Streaking Repetition
Figure:1.1: Steps of streak plate method. 1
Figure:1.2: Procedure of Streak Plate Method.
plate and pour plate method
The plate-based method was first developed in the 1970s in Sweden and has been
integrated into public health nutrition tools of many countries, including the 2019
Canada's Food Guide and the United States'. A bacterial culture was spread on the glass
slide, then placed in the moist chamber with a small wet paper. Bacterial growth was
easily visible. Koch publicly demonstrated his plating method at the Seventh
International Medical Congress in London in August 1881. Since the development of the
agar plate in Robert Koch's laboratory, several methods have been used to achieve an
even distribution of bacterial growth on or in the agar. The most common methods used
to achieve this type of distribution are: streak, pour, thin-layer, spread and membrane
filter method.
WHAT IS STREAK PLATE METHOD?
The streak-plate procedure is designed to isolate pure cultures of bacteria, or colonies, from
mixed populations by simple mechanical separation. It involves spreading a small and
concentrated sample of microorganisms over the surface of a solid agar medium in a way that the
cells are progressively diluted and separated from each other. Single colonies are comprised of
millions of cells growing in a cluster on or within an agar plate. A colony, unlike a single cell, is
visible to the naked eye. It is commonly employed in environmental microbiology, clinical
microbiology, and research laboratories. Here is a step-by-step description of the streak plate
method:
, Inoculation Streaking Repetition
Figure:1.1: Steps of streak plate method. 1
Figure:1.2: Procedure of Streak Plate Method.
