Biosafety & Biosecurity Study Guide
Exam And Actual Answers.
BMBL - Answer Biosafety in Microbiological and Biomedical Laboratories
a set of guidelines for the safe handling and containment of biological agents
-established by the CDC and NIH
Biological Agents - Answer Infectious microorganisms and potentially hazardous biological materials
Risk Assessment - Answer process that enables the appropriate selection of microbiological practices,
safety equipment, and facility safeguards that can prevent laboratory-associated infections (LAIs)
LAI - Answer Laboratory-Associated Infections
How has the rate of Laboratory-Associated Infections decreased? - Answer Improvements in
containment equipment, engineering controls, and a greater emphasis on safety training
What are the primary risk criteria - Answer • Infectivity
• Severity of disease
• Transmissibility
• Nature of the work being conducted
• Agent origin - for agents that cause moderate to severe disease
What are common ways of developing LAIs? - Answer exposure to contaminated aerosols and droplets
Biological Risk Assessment - Answer • Staff's training and technical experience
• Activities that might lead to staff exposure
• Probability that agent exposure will lead to a LAI
• Availability of preventive measures and effective treatments for the disease
, • Consequences of an infection
How are infectious agents classified by NIH? - Answer into ascending risk groups from 1-4
-higher # indicates higher risk of hazardous characteristics that may cause human disease
How are infectious agents classified by WHO? - Answer based on degree of Dz hazard to humans and
animals, natural route of the Dz's transmission, preventative measures, and effective treatments
WHO - Answer World Health Organization
Genetically Modified Agent Hazards - Answer possibility that the genetic modification could increase an
agent's pathogenicity or affect its susceptibility to effective tx
Dangers of Aerosols - Answer typically undetected and are extremely pervasive
-those containing infectious particles are hazardous even when inhalation may not be a natural route of
transmission of disease
Most important element of containment - Answer strict adherence to standard microbiological
practices and techniques
What are examples of primary barriers? - Answer Safety Equipment and PPE:
-Biological Safety Cabinet
-gloves, safety glasses, gowns, shoe covers
• Class I and II BSC - Answer protection to both the lab personnel and environment when used with
good microbiological practices
Class II & III BSC - Answer protects research materials from airborne contaminants by using a HEPA
filter
BSC class 1 - Answer Personnel and Environmental Protection only!
Exam And Actual Answers.
BMBL - Answer Biosafety in Microbiological and Biomedical Laboratories
a set of guidelines for the safe handling and containment of biological agents
-established by the CDC and NIH
Biological Agents - Answer Infectious microorganisms and potentially hazardous biological materials
Risk Assessment - Answer process that enables the appropriate selection of microbiological practices,
safety equipment, and facility safeguards that can prevent laboratory-associated infections (LAIs)
LAI - Answer Laboratory-Associated Infections
How has the rate of Laboratory-Associated Infections decreased? - Answer Improvements in
containment equipment, engineering controls, and a greater emphasis on safety training
What are the primary risk criteria - Answer • Infectivity
• Severity of disease
• Transmissibility
• Nature of the work being conducted
• Agent origin - for agents that cause moderate to severe disease
What are common ways of developing LAIs? - Answer exposure to contaminated aerosols and droplets
Biological Risk Assessment - Answer • Staff's training and technical experience
• Activities that might lead to staff exposure
• Probability that agent exposure will lead to a LAI
• Availability of preventive measures and effective treatments for the disease
, • Consequences of an infection
How are infectious agents classified by NIH? - Answer into ascending risk groups from 1-4
-higher # indicates higher risk of hazardous characteristics that may cause human disease
How are infectious agents classified by WHO? - Answer based on degree of Dz hazard to humans and
animals, natural route of the Dz's transmission, preventative measures, and effective treatments
WHO - Answer World Health Organization
Genetically Modified Agent Hazards - Answer possibility that the genetic modification could increase an
agent's pathogenicity or affect its susceptibility to effective tx
Dangers of Aerosols - Answer typically undetected and are extremely pervasive
-those containing infectious particles are hazardous even when inhalation may not be a natural route of
transmission of disease
Most important element of containment - Answer strict adherence to standard microbiological
practices and techniques
What are examples of primary barriers? - Answer Safety Equipment and PPE:
-Biological Safety Cabinet
-gloves, safety glasses, gowns, shoe covers
• Class I and II BSC - Answer protection to both the lab personnel and environment when used with
good microbiological practices
Class II & III BSC - Answer protects research materials from airborne contaminants by using a HEPA
filter
BSC class 1 - Answer Personnel and Environmental Protection only!
