ASCP MLS LATEST(2 DIFFERENT VERSIONS) 210 REAL EXAM
QUESTIONS AND CORRECT ANSWERS
Antibody most associated with Delayed hemolytic transfusion reactions - ANSWER:
Anti-Jk is associated
Antibody associated with Mycoplasma pneumonia - ANSWER: Anti-I is associated
Antibody associated with the Ebstein barr virus - ANSWER: Anti-i is associated
Immunoglobulin that causes extra-vascular hemolisys - ANSWER: Immunoglobulin
IgG
Immunoglobulin that causes intra-vascular hemolisys - ANSWER: Immunoglobulin
IgM
Enzyme treatment of these antigens will improve reactivity - ANSWER: rh, kidd, ii,
lewis
Enzyme treatment of these antigens will remove reactivity: - ANSWER: duffy, mnss
antigens damaged/destroyed by ZZAP - ANSWER: Kell antigens, M, N, and the two
main Duffy antigens (Fya and Fyb)
Temp for whole blood storage - ANSWER: 1-6 celsius
CPDA-1 storage date - ANSWER: 35 days of storage
Adsol storage date - ANSWER: 42 days of storage
Frozen RBC storage - ANSWER: -80 degrees Celsius
How long can you store Frozen RBCs - ANSWER: 10 years
How long after thawing can a unit of RBCs be stored for? - ANSWER: 24 hrs
How long are irradiated RBCs be stored for? - ANSWER: 28 days or exp date
(Whichever is closer)
How long can a unit of apheresis /random platelet store for? - ANSWER: 5 days @
room temp
How long is storage for Granulocytes? - ANSWER: 24 hrs @ room temp
How long is storage for FFP? - ANSWER: 1 year at -18 degrees Celsius
,How long is the storage for Cryo? - ANSWER: 1 year @ -18 degrees Celsius
Factor VIII, AHF, Factor IX and albumin can be stored at? - ANSWER: 1-6 degrees
Celsius
What does cryoprecipitate contain? - ANSWER: Fibrinogen, factor VIII, factor XIII,
vWF, and fibronectin
What is the most antgenic with 85% - ANSWER: D antigen
What is the second most antigenic with 9% - ANSWER: Big K antigen
What are the requirements for a intrauterine transfusion? - ANSWER: Fresh (less
than 5 days old),l CMV (=), irradiated and collected with CDPA. NOT ADSOL.
When is FFP used? - ANSWER: When PT and PTT are prolonged
Room temperature reacting antibodies - ANSWER: Lewis, MNSs and P blood group
systems (anti-Lea, anti-M, and anti-P1)
AHG phase reacting antibodies - ANSWER: Duffy, Kidd, and Kell blood group systems
(anti-Fya, anti-Jka, and anti-K)
37 degree reacting antibodies - ANSWER: Rh system (anti-E, anti-D, anti-e, anti-C,
and anti-c)
What kind of immunoglobulin is a "P" and what does it follow? - ANSWER: IgA
Biphasic
follows infections in children
Why must blood from a mother to a child be irradiated? - ANSWER: To prevent
transfusion reaction graft vs host disease from the donor lymphs in the blood.
What does a room temp cross match detect? - ANSWER: it detects ABO errors
What does AHG detect? - ANSWER: it detects allo/auto andibodies
Why would a premature infant need a blood transfusion? - ANSWER: mostly due to
blood loss from lab tests
What kind of positive control is important when phenotyping? - ANSWER:
heterozygously positive control
ex: when testing for a Duffy Fya, use the cells that are (+)Fya (+) Fyb
A positive Auto control can be caused by? - ANSWER: Rouleaux and a cold auto
,Saline replacement technique is useful in resolving ________ in blood bank? -
ANSWER: rouleaux
Warming a specimen is useful in resolving ________ in blood bank? - ANSWER: a
cold auto
A cold auto will react with...? - ANSWER: polyspecific AHG and C3 monospecific AHG
reasons to defer donor for 1 year - ANSWER: Received blood products
If you live with or have had sexual contact with a person who has hepatitis
Hep B globulin
reasons to defer donor for 3 months - ANSWER: syphilis
gonorrhea
reasons for a permanent donor defer - ANSWER: Drugs
chronic disorders
babesiosis, trypanosoma, chagas
Pituitary growth hormone
Donor requirements - ANSWER: AGE:17 and up
Temp: less than or = 99.5
weight: 110
Hct: greater than or = to 38% (33% for autologus)
Pluse: 50-100 beats per min
Blood pressure: less than 180/100
Results of a patient in DIC - ANSWER: ↑PT, ↑ PTT, ↑TT ↑FSP ↓Fbg and ↓Plt count
(+) D-Dimer
presence of schistocytes
What does PT monitor? - ANSWER: Coumadin therapy (warfrin)
What is a normal range for a PT - ANSWER: 9-12.5
What is the therapeutic range for PT - ANSWER: 2-3x the normal range
What does PTT monitor? - ANSWER: Heparin therapy
What is the normal range for PTT? - ANSWER: 20-35 seconds
What is the therapeutic range for PTT? - ANSWER: 1.2-2 X the normal range
What is one of the only parameters that is not affected by cold agglutinin? -
ANSWER: The Hemoglobin
Interfering substances for: WBC - ANSWER: clumped plts
, NRBCs
Interfering substances for: RBC - ANSWER: ↑ WBC
RBC fragments
clumped cells (agglutination)
Interfering substances for: Hgb - ANSWER: Lipemic
Icteric
↑ WBCs
Interfering substances for: MCV - ANSWER: Clumped cells
↑ glucose
↑ WBCs
What does Protein C deficiency cause? - ANSWER: Thrombosis
What does Protein C inhibit? - ANSWER: Va and VIIIa
What factor deficiency causes Hemophilia A? - ANSWER: Factor VIII
What factor deficiency causes thrombosis and has an elevated PTT? - ANSWER:
Factor XII (Hageman factor)
If a mixing study is done on a patient with an elevated PTT with normal plasma and it
corrects itself, what is the problem? - ANSWER: A factor deficiency
If a mixing study is done on a patient with an elevated PTT with normal plasma and it
does NOT corrects itself, what is the problem? - ANSWER: A Lupus antcoagulant
How is a Lupus anticoagulant confirmed? - ANSWER: With tissue thromboplastic
inhibitor test with Plt neutralization procedure prep
Vitamin K factors - ANSWER: II, VII, IX, X
Consumable factors - ANSWER: I, V, VIII, XIII (consumed in clot; not in serum)
Common pathway factors - ANSWER: I, II, V, X
labile factors - ANSWER: V and VIII
Contact factors - ANSWER: XI, XII, Pre-Kallikrein
intrinsic factor - ANSWER: XII, XI, IX, VIII
extrinsic factors - ANSWER: VII
Normal plasma - ANSWER: All factors
QUESTIONS AND CORRECT ANSWERS
Antibody most associated with Delayed hemolytic transfusion reactions - ANSWER:
Anti-Jk is associated
Antibody associated with Mycoplasma pneumonia - ANSWER: Anti-I is associated
Antibody associated with the Ebstein barr virus - ANSWER: Anti-i is associated
Immunoglobulin that causes extra-vascular hemolisys - ANSWER: Immunoglobulin
IgG
Immunoglobulin that causes intra-vascular hemolisys - ANSWER: Immunoglobulin
IgM
Enzyme treatment of these antigens will improve reactivity - ANSWER: rh, kidd, ii,
lewis
Enzyme treatment of these antigens will remove reactivity: - ANSWER: duffy, mnss
antigens damaged/destroyed by ZZAP - ANSWER: Kell antigens, M, N, and the two
main Duffy antigens (Fya and Fyb)
Temp for whole blood storage - ANSWER: 1-6 celsius
CPDA-1 storage date - ANSWER: 35 days of storage
Adsol storage date - ANSWER: 42 days of storage
Frozen RBC storage - ANSWER: -80 degrees Celsius
How long can you store Frozen RBCs - ANSWER: 10 years
How long after thawing can a unit of RBCs be stored for? - ANSWER: 24 hrs
How long are irradiated RBCs be stored for? - ANSWER: 28 days or exp date
(Whichever is closer)
How long can a unit of apheresis /random platelet store for? - ANSWER: 5 days @
room temp
How long is storage for Granulocytes? - ANSWER: 24 hrs @ room temp
How long is storage for FFP? - ANSWER: 1 year at -18 degrees Celsius
,How long is the storage for Cryo? - ANSWER: 1 year @ -18 degrees Celsius
Factor VIII, AHF, Factor IX and albumin can be stored at? - ANSWER: 1-6 degrees
Celsius
What does cryoprecipitate contain? - ANSWER: Fibrinogen, factor VIII, factor XIII,
vWF, and fibronectin
What is the most antgenic with 85% - ANSWER: D antigen
What is the second most antigenic with 9% - ANSWER: Big K antigen
What are the requirements for a intrauterine transfusion? - ANSWER: Fresh (less
than 5 days old),l CMV (=), irradiated and collected with CDPA. NOT ADSOL.
When is FFP used? - ANSWER: When PT and PTT are prolonged
Room temperature reacting antibodies - ANSWER: Lewis, MNSs and P blood group
systems (anti-Lea, anti-M, and anti-P1)
AHG phase reacting antibodies - ANSWER: Duffy, Kidd, and Kell blood group systems
(anti-Fya, anti-Jka, and anti-K)
37 degree reacting antibodies - ANSWER: Rh system (anti-E, anti-D, anti-e, anti-C,
and anti-c)
What kind of immunoglobulin is a "P" and what does it follow? - ANSWER: IgA
Biphasic
follows infections in children
Why must blood from a mother to a child be irradiated? - ANSWER: To prevent
transfusion reaction graft vs host disease from the donor lymphs in the blood.
What does a room temp cross match detect? - ANSWER: it detects ABO errors
What does AHG detect? - ANSWER: it detects allo/auto andibodies
Why would a premature infant need a blood transfusion? - ANSWER: mostly due to
blood loss from lab tests
What kind of positive control is important when phenotyping? - ANSWER:
heterozygously positive control
ex: when testing for a Duffy Fya, use the cells that are (+)Fya (+) Fyb
A positive Auto control can be caused by? - ANSWER: Rouleaux and a cold auto
,Saline replacement technique is useful in resolving ________ in blood bank? -
ANSWER: rouleaux
Warming a specimen is useful in resolving ________ in blood bank? - ANSWER: a
cold auto
A cold auto will react with...? - ANSWER: polyspecific AHG and C3 monospecific AHG
reasons to defer donor for 1 year - ANSWER: Received blood products
If you live with or have had sexual contact with a person who has hepatitis
Hep B globulin
reasons to defer donor for 3 months - ANSWER: syphilis
gonorrhea
reasons for a permanent donor defer - ANSWER: Drugs
chronic disorders
babesiosis, trypanosoma, chagas
Pituitary growth hormone
Donor requirements - ANSWER: AGE:17 and up
Temp: less than or = 99.5
weight: 110
Hct: greater than or = to 38% (33% for autologus)
Pluse: 50-100 beats per min
Blood pressure: less than 180/100
Results of a patient in DIC - ANSWER: ↑PT, ↑ PTT, ↑TT ↑FSP ↓Fbg and ↓Plt count
(+) D-Dimer
presence of schistocytes
What does PT monitor? - ANSWER: Coumadin therapy (warfrin)
What is a normal range for a PT - ANSWER: 9-12.5
What is the therapeutic range for PT - ANSWER: 2-3x the normal range
What does PTT monitor? - ANSWER: Heparin therapy
What is the normal range for PTT? - ANSWER: 20-35 seconds
What is the therapeutic range for PTT? - ANSWER: 1.2-2 X the normal range
What is one of the only parameters that is not affected by cold agglutinin? -
ANSWER: The Hemoglobin
Interfering substances for: WBC - ANSWER: clumped plts
, NRBCs
Interfering substances for: RBC - ANSWER: ↑ WBC
RBC fragments
clumped cells (agglutination)
Interfering substances for: Hgb - ANSWER: Lipemic
Icteric
↑ WBCs
Interfering substances for: MCV - ANSWER: Clumped cells
↑ glucose
↑ WBCs
What does Protein C deficiency cause? - ANSWER: Thrombosis
What does Protein C inhibit? - ANSWER: Va and VIIIa
What factor deficiency causes Hemophilia A? - ANSWER: Factor VIII
What factor deficiency causes thrombosis and has an elevated PTT? - ANSWER:
Factor XII (Hageman factor)
If a mixing study is done on a patient with an elevated PTT with normal plasma and it
corrects itself, what is the problem? - ANSWER: A factor deficiency
If a mixing study is done on a patient with an elevated PTT with normal plasma and it
does NOT corrects itself, what is the problem? - ANSWER: A Lupus antcoagulant
How is a Lupus anticoagulant confirmed? - ANSWER: With tissue thromboplastic
inhibitor test with Plt neutralization procedure prep
Vitamin K factors - ANSWER: II, VII, IX, X
Consumable factors - ANSWER: I, V, VIII, XIII (consumed in clot; not in serum)
Common pathway factors - ANSWER: I, II, V, X
labile factors - ANSWER: V and VIII
Contact factors - ANSWER: XI, XII, Pre-Kallikrein
intrinsic factor - ANSWER: XII, XI, IX, VIII
extrinsic factors - ANSWER: VII
Normal plasma - ANSWER: All factors
