Name: Score:
66 Multiple choice questions
Term 1 of 66
Match the type of symbiotic relationship that best describes the host-microbe interaction in the
body:
Staphylococcus epidermidis obtains nutrients from the skin without causing harm
mutualism
parasitism
commensalism
Term 2 of 66
When cloning using the pUC19 vector, how can you confirm that a bacterial cell has successfully
taken up the plasmid with DNA inserted?
Look for white colonies growing in the presence of ampicillin
Look for blue colonies growing in the presence of ampicillin
Look for white colonies growing in the absence of ampicillin
Look for blue colonies growing in the absence of ampicillin
Term 3 of 66
At what life stage is the human microbiome thought to begin developing?
at birth
at puberty
at middle-age
,Term 4 of 66
Biofilm formation is under the control of what regulatory system?
catabolite repression
quorum sensing
repression of the lac operon
Term 5 of 66
Which hypothesis of the endosymbiotic origin of eukaryotes suggests that mitochondria were
derived from an endosymbiosis that occurred in an archaeal cell,facilitating the evolution of cell
complexity?
Serial endosymbiosis
Symbiogenesis
Term 6 of 66
(first) What marine phototroph is thought to be responsible for ~50% of net primaryproduction?
Ostreococcus
Trichodesmium
Prochlorococcus
Roseofilum
, Term 7 of 66
You are cloning using the pUC19 vector. After transforming E. coli cells and plating them in the
presence of ampicillin, you observe only white colonies on your plate?Which of the following is a
possible explanation for this observation? (choose all that apply)
a. You forgot to digest the vector with the same restriction enzyme as your DNA insert
b. You forgot to add X-Gal to your plate
c. All the E. coli cells took up plasmid containing the DNA insert
d. All the E. coli cells took up plasmid that did not contain the DNA insert
a,b,d
a
b,c
b
Term 8 of 66
You have been assigned the task of designing an experiment to answer the question "what
bacterial species are present in the microbial community of a sample of pond water taken on
campus?". Which type of 'omics would be most appropriate in your experimental design?
Genomics
Metagenomics
Transcriptomics
Proteomics
Term 9 of 66
All the following are key processes in the sulfur cycle EXCEPT
Desulfurylation
Sulfide oxidation
Ammonification
Sulfate reduction
66 Multiple choice questions
Term 1 of 66
Match the type of symbiotic relationship that best describes the host-microbe interaction in the
body:
Staphylococcus epidermidis obtains nutrients from the skin without causing harm
mutualism
parasitism
commensalism
Term 2 of 66
When cloning using the pUC19 vector, how can you confirm that a bacterial cell has successfully
taken up the plasmid with DNA inserted?
Look for white colonies growing in the presence of ampicillin
Look for blue colonies growing in the presence of ampicillin
Look for white colonies growing in the absence of ampicillin
Look for blue colonies growing in the absence of ampicillin
Term 3 of 66
At what life stage is the human microbiome thought to begin developing?
at birth
at puberty
at middle-age
,Term 4 of 66
Biofilm formation is under the control of what regulatory system?
catabolite repression
quorum sensing
repression of the lac operon
Term 5 of 66
Which hypothesis of the endosymbiotic origin of eukaryotes suggests that mitochondria were
derived from an endosymbiosis that occurred in an archaeal cell,facilitating the evolution of cell
complexity?
Serial endosymbiosis
Symbiogenesis
Term 6 of 66
(first) What marine phototroph is thought to be responsible for ~50% of net primaryproduction?
Ostreococcus
Trichodesmium
Prochlorococcus
Roseofilum
, Term 7 of 66
You are cloning using the pUC19 vector. After transforming E. coli cells and plating them in the
presence of ampicillin, you observe only white colonies on your plate?Which of the following is a
possible explanation for this observation? (choose all that apply)
a. You forgot to digest the vector with the same restriction enzyme as your DNA insert
b. You forgot to add X-Gal to your plate
c. All the E. coli cells took up plasmid containing the DNA insert
d. All the E. coli cells took up plasmid that did not contain the DNA insert
a,b,d
a
b,c
b
Term 8 of 66
You have been assigned the task of designing an experiment to answer the question "what
bacterial species are present in the microbial community of a sample of pond water taken on
campus?". Which type of 'omics would be most appropriate in your experimental design?
Genomics
Metagenomics
Transcriptomics
Proteomics
Term 9 of 66
All the following are key processes in the sulfur cycle EXCEPT
Desulfurylation
Sulfide oxidation
Ammonification
Sulfate reduction
