GEL ELECTROPHORESIS EXAM QUESTIONS WITH 100% CORRECT ANSWERS { GRADED A+}

GEL ELECTROPHORESIS EXAM

QUESTIONS WITH 100% CORRECT

ANSWERS { GRADED A+}




What is the purpose of gel electrophoresis? - ✔✔To separate DNA

fragments based on their size using electricity


What does "phoresis" mean? - ✔✔To transmit (move location, migrate)


What ingredients make up a gel? - ✔✔Agarose & buffer


What is agarose? - ✔✔A sugar found in seaweed


What is a characteristic of agarose? - ✔✔It is gelatin-like


What purpose does agarose serve in gel electrophoresis? - ✔✔It is the

matrix (obstacle course) for DNA fragments (it separates them by size)

, What is the buffer? - ✔✔A salt solution


What purpose does the buffer serve in gel electrophoresis? - ✔✔It

maintains pH (at 7 which is neutral) and conducts electricity which

allows the DNA fragments to migrate through the agarose gel

What purpose does the distilled H2O serve in gel electrophoresis? -

✔✔It is the solvent


Why does the H2O need to be distilled? - ✔✔It contains no chlorine or

other contaminants that could damage DNA


What equipment is required for gel electrophoresis? - ✔✔Casting tray

(baby gates, combs), chambers, lids, power sources


What is the process of gel electrophoresis? - ✔✔The DNA is loaded into

wells formed by combs in the agarose gel, buffer is added, then an

electric current is applied and the DNA migrates to the bottom