MICROBIOLOGY EXAM REVIEWED QUESTIONS AND THEIR 100% CORRECT ANSWERS

MICROBIOLOGY EXAM REVIEWED
QUESTIONS AND THEIR 100%
CORRECT ANSWERS

B
1) The following steps are used to
make DNA fingerprints. What is
the third step?
A) Collect DNA.
B) Digest with a restriction
enzyme.
C) Perform electrophoresis.
D) Lyse cells.
E) Add stain.
B
2) How many pieces will EcoRI
produce from the plasmid shown
in Figure 9.1?
A) 1

,B) 2
C) 3
D) 4
E) 5
D
3) In Figure 9.1, after digestion
with the appropriate restriction
enzyme, what is the smallest
piece containing the ampicillin-
resistance (amp) gene?
A) 0.17 kilobase pairs
B) 0.25 kbp
C) 1.08 kbp
D) 1.50 kbp
E) 3.00 kbp
C
4) In Figure 9.2, the enzyme in
step 1 is
A) DNA polymerase.

,B) DNA ligase.
C) RNA polymerase.
D) Reverse transcriptase.
E) Spliceosome.
A
5) In Figure 9.2, the enzyme in
step 2 is
A) DNA polymerase.
B) DNA ligase.
C) RNA polymerase.
D) Reverse transcriptase.
E) Spliceosome.
B
6) The reaction catalyzed by DNA
polymerase is
A) DNA → mRNA
B) mRNA → cDNA
C) mRNA → protein

, D) DNA → DNA
E) tRNA → mRNA
C
7) Which of the following is an
advantage of using E. coli to
make a human gene product?
A) Endotoxin may be in the
product.
B) It doesn't secrete most
proteins.
C) Its genes are well known.
D) It can't process introns.
E) None of the above.
E
8) Which of the following is NOT
an agricultural product made by
DNA techniques?
A) Frost retardant
B) Bacillus thuringiensis