PHA 614 | LABORATORY
1F-PH ‘28 |
1st Semester | 2nd Shifting |A.Y. 2024-2025
MEASURING VOLUME APPARATUS
VOLUME MEASUREMENTS
Graduated Cylinder or Cylindrical
➔ Ideally, conducted at 20℃.
Graduates
➔ Proper handling techniques are also
necessary
➔ Factors to consider:
1. Temperature of apparatus
2. Material being used
3. Solvents used to prepare solutions
4. Area in which they are prepared
➔ measuring liquid volumes in
5. Final volume adjustment
routine experiments
Terminology
➔ Displacement determination
★ Volume Measurements in Pharmacy ★ Graduated Cylinder/ Cylindrical
Graduates Measurements
➔ Preparation of solutions of the
➔ 10 mL
correct concentration
➔ 25 mL
➔ Assay of drugs in pharmaceutical ➔ 50 mL
dosage forms ➔ 100 mL
➔ 500 mL
➔ Preparation of culture media and ➔ 1000 mL
broth ➔
➔ HOW TO USE THE MENISCUS
➔ Preparation of drug solutions in
1. Determine the meniscus of the
determining its minimum inhibitory
sample.
concentration that can inhibit the
2. Position the instrument
growth of a specific microorganism
appropriately aligned with the eye
level.
3. Determine the scale to which the
meniscus is placed.
4. Record the measurement.
, LABORATORY # | PHA 614 - 1FPH
(Always measure the level at the
bottom of the curve *meniscus*)
➔ Lower meniscus – clear liquids (can
be colored)
➔ Upper meniscus – dark-colored
liquids
Pipettes
Syringe
➔ Delivering single or specific volumes
➔ Measuring for viscous liquids
➔ Used for filtering solution
★ Measuring /Calibrated pipettes
➔ Several marks, deliver a specific
volume.
1F-PH ‘28 |
1st Semester | 2nd Shifting |A.Y. 2024-2025
MEASURING VOLUME APPARATUS
VOLUME MEASUREMENTS
Graduated Cylinder or Cylindrical
➔ Ideally, conducted at 20℃.
Graduates
➔ Proper handling techniques are also
necessary
➔ Factors to consider:
1. Temperature of apparatus
2. Material being used
3. Solvents used to prepare solutions
4. Area in which they are prepared
➔ measuring liquid volumes in
5. Final volume adjustment
routine experiments
Terminology
➔ Displacement determination
★ Volume Measurements in Pharmacy ★ Graduated Cylinder/ Cylindrical
Graduates Measurements
➔ Preparation of solutions of the
➔ 10 mL
correct concentration
➔ 25 mL
➔ Assay of drugs in pharmaceutical ➔ 50 mL
dosage forms ➔ 100 mL
➔ 500 mL
➔ Preparation of culture media and ➔ 1000 mL
broth ➔
➔ HOW TO USE THE MENISCUS
➔ Preparation of drug solutions in
1. Determine the meniscus of the
determining its minimum inhibitory
sample.
concentration that can inhibit the
2. Position the instrument
growth of a specific microorganism
appropriately aligned with the eye
level.
3. Determine the scale to which the
meniscus is placed.
4. Record the measurement.
, LABORATORY # | PHA 614 - 1FPH
(Always measure the level at the
bottom of the curve *meniscus*)
➔ Lower meniscus – clear liquids (can
be colored)
➔ Upper meniscus – dark-colored
liquids
Pipettes
Syringe
➔ Delivering single or specific volumes
➔ Measuring for viscous liquids
➔ Used for filtering solution
★ Measuring /Calibrated pipettes
➔ Several marks, deliver a specific
volume.
