Rutgers Intro to Biochem-Murphy Module 2 Practice Questions and Answers

Rutgers Intro to Biochem-Murphy Module 2 Practice Questions and Answers What is the isoelectric point of the following tetrapetide that contains the amino acids represented by the single letters "DREN", such that D is at the amino-terminal end of the peptide and N is at the carboy- terminal end. 3.86 4.06 6.53 8.8 12.48 - Ans:-4.06 What is the function of a lyase enzyme. To assist the substrate by binding to the enzyme, enabling substrate to active site engagement to facilitate a reaction of one substrate to form two products with the use of water to facilitate a reaction of one substrate to form two products without the use of water ©GRACEAMELIA 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED FIRST PUBLISH OCTOBER 2024 Page 2/18 To tell fibs - Ans:-to facilitate a reaction of one substrate to form two products without the use of water If the free energy change ΔG for a reaction is -46.11 kJ/mol, the reaction is: at equilibrium. endergonic. endothermic. exergonic. exothermic. - Ans:-exergonic. Determine the net charge of the following tetrapeptide at pH 13.5 that contains the amino acids represented by the single letters "MRPH", such that M is at the amino-terminal end of the peptide and H is at the carboy-terminal end. +2 +1 0 -1 ©GRACEAMELIA 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED FIRST PUBLISH OCTOBER 2024 Page 3/18 -2 - Ans:--1 Local folding of a protein occurs at what defined level of protein folding? Primary Secondary Tertiary Quarternary All of the above - Ans:-Secondary Determine the net charge of the following tetrapeptide at pH 3 that contains the amino acids represented by the single letters "KYLE", such that K is at the amino-terminal end of the peptide and E is at the carboy-terminal end. +2 +1 0 -1 -2 - Ans:-+1 ©GRACEAMELIA 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED FIRST PUBLISH OCTOBER 2024 Page 4/18 What is the isoelectric point of the following tetrapetide that contains the amino acids represented by the single letters "HPRM", such that H is at the amino-terminal end of the peptide and M is at the carboy-terminal end. 10.83 10.85 7.59 7.61 1.24 - Ans:-10.83 Which of the following amino acids contain a hydrophobic side chain Leucine Arginine Glutamine Glutamic acid Aspartic acid - Ans:-Leucine ©GRACEAMELIA 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED FIRST PUBLISH OCTOBER 2024 Page 5/18 The value that is altered when you compare an enzyme catalyzed reaction to a non-enzyme catalyzed reaction is called. Ea delta G delta G double dagger delta G naught - Ans:-Ea What is a common role of a coenzyme in a reaction as stated in class To lower the activation energy of a reaction further The donation of atoms to a reaction To alter the enzymes shape To assist the substrate by binding to the enzyme, enabling substrate to active site engagement - Ans:- The donation of atoms to a reaction Determine the type of inhibition that has occurred in a first order Michaelis-Menten enzyme catalyzed reaction that has yielded the following data. Vi is the velocity in the presence of inhibitor, V is the velocity when run without inhibitor and [S] refers to the substrate concentration. [S] V Vi ©GRACEAMELIA 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED FIRST PUBLISH OCTOBER 2024 Page 6/18 3.333 0.283 0.055 1.000 0.260 0.054 0.400 0.222 0.052 0.263 0.197 0.051 0.179 0.171 0.049 Competitive Non-competitive Uncompetitive No inhibition at all None of the above - Ans:-Uncompetitive Fill in all that apply. When looking at a double reciprocal graph of data from an uncompetitive enzyme compared to data from a competitive enzyme, which of the two properties will show up on the graph as different? Correct! Km ©GRACEAMELIA 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED FIRST PUBLISH OCTOBER 2024 Page 7/18 total enzyme concentration substrate concentration Correct! Vmax time - Ans:-Km Vmax The following image shows a standard amino acid. Which bond is a psi bond? A B C D - Ans:-C - just trust me its C ©GRACEAMELIA 2024/