©GRACEAMELIA 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED
FIRST PUBLISH OCTOBER 2024
BIOL 301 Exam 1 Practice Questions and
Answers
What can distinguish between RNA and DNA? - Ans:✔✔-Presence of 2' OH on RNA
A nucleosome is composed of how many total histone molecules? - Ans:✔✔-8
In terms of MNase digestion, a chromatosome protects ___ base pairs, while a nucelosome protects ___
base pairs. - Ans:✔✔-166, 146
Adenine binds to Thymine by ___ hydrogen bonds, while Cytosine binds to Guanine with ___ hydrogen
bonds. - Ans:✔✔-2, 3
The special sequence of centromeres provide what function? - Ans:✔✔-Site for spindle fiber attachment.
If it is known that a double-stranded DNA sequence is 20% adenine, what percentage is Guanine? -
Ans:✔✔-30%
In B-form DNA (the typical form of DNA in our cells), how many base-pairs are present in one helical turn
of the DNA? - Ans:✔✔-10
Page 1/6
, ©GRACEAMELIA 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED
FIRST PUBLISH OCTOBER 2024
Who used DNase, RNase, and protease to prove that DNA was the "transforming agent" of
Strepococcus? - Ans:✔✔-Avery, MacLeod, and McCarty
Who collected data on base rations (purines equal pyrimidines)? - Ans:✔✔-Chargaff
Who built the first accurate model for the structure of DNA? - Ans:✔✔-Watson and Crick
Who transformed Type IIR bacteria into Type IIS bacteria in mice? - Ans:✔✔-Griffith
Who used 32P and 35S to show the genetic information in bacteriophage T2? - Ans:✔✔-Hershey and
Chase
What are the purines? - Ans:✔✔-Adenine and Guanine
What are the pyrimidines? - Ans:✔✔-Cytosine and Thymine
Who performed denaturation mapping of phage DNA to demonstrate bidirectional replication? -
Ans:✔✔-Schnos and Inman
What enzyme in prokaryotes lays down the RNA primers on the lagging strand? - Ans:✔✔-DNA primase
Polymerases that "back up" to remove bases that were incorporated incorrectly are utilizing what
activity? - Ans:✔✔-3' to 5' exonuclease activity
Which enzyme extends the DNA at chromosomal ends? - Ans:✔✔-Telomerase
Page 2/6
FIRST PUBLISH OCTOBER 2024
BIOL 301 Exam 1 Practice Questions and
Answers
What can distinguish between RNA and DNA? - Ans:✔✔-Presence of 2' OH on RNA
A nucleosome is composed of how many total histone molecules? - Ans:✔✔-8
In terms of MNase digestion, a chromatosome protects ___ base pairs, while a nucelosome protects ___
base pairs. - Ans:✔✔-166, 146
Adenine binds to Thymine by ___ hydrogen bonds, while Cytosine binds to Guanine with ___ hydrogen
bonds. - Ans:✔✔-2, 3
The special sequence of centromeres provide what function? - Ans:✔✔-Site for spindle fiber attachment.
If it is known that a double-stranded DNA sequence is 20% adenine, what percentage is Guanine? -
Ans:✔✔-30%
In B-form DNA (the typical form of DNA in our cells), how many base-pairs are present in one helical turn
of the DNA? - Ans:✔✔-10
Page 1/6
, ©GRACEAMELIA 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED
FIRST PUBLISH OCTOBER 2024
Who used DNase, RNase, and protease to prove that DNA was the "transforming agent" of
Strepococcus? - Ans:✔✔-Avery, MacLeod, and McCarty
Who collected data on base rations (purines equal pyrimidines)? - Ans:✔✔-Chargaff
Who built the first accurate model for the structure of DNA? - Ans:✔✔-Watson and Crick
Who transformed Type IIR bacteria into Type IIS bacteria in mice? - Ans:✔✔-Griffith
Who used 32P and 35S to show the genetic information in bacteriophage T2? - Ans:✔✔-Hershey and
Chase
What are the purines? - Ans:✔✔-Adenine and Guanine
What are the pyrimidines? - Ans:✔✔-Cytosine and Thymine
Who performed denaturation mapping of phage DNA to demonstrate bidirectional replication? -
Ans:✔✔-Schnos and Inman
What enzyme in prokaryotes lays down the RNA primers on the lagging strand? - Ans:✔✔-DNA primase
Polymerases that "back up" to remove bases that were incorporated incorrectly are utilizing what
activity? - Ans:✔✔-3' to 5' exonuclease activity
Which enzyme extends the DNA at chromosomal ends? - Ans:✔✔-Telomerase
Page 2/6
