©GRACEAMELIA 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED
FIRST PUBLISH OCTOBER 2024
Biology 116 Final Exam Questions and
Answers
Determination of what DNA is made of - Ans:✔✔-Hershey and Chase marked Virus's with two different
radioactive molecules (32p labels DNA, 35s labels protein)
32p was found only inside of infected cells
35s was found in ghost shells
Nucleic Acid - Ans:✔✔-polymer of nucleotide monomer
Nucleotide - Ans:✔✔-phosphate group+ sugar+ base
synthesized from nucleoside triphosphates (NTPs)
Nucleoside - Ans:✔✔-sugar + base
Ribose - Ans:✔✔-found in RNA
contains additional O on 2' end
Deoxyribose - Ans:✔✔-found in DNA
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, ©GRACEAMELIA 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED
FIRST PUBLISH OCTOBER 2024
no O on 2' end
Purines - Ans:✔✔-Guanine and Adenine (double ring)
Pyrimidines - Ans:✔✔-Cytosine Uracil and Thymine (single ring)
Polymerization of of Nucleotides - Ans:✔✔-3' hydroxyl group in the sugar links with the phosphate group
of another nucleotide
Dehydration reaction
Polarity of Nucleic acids - Ans:✔✔-has polarity extending from free 5' to 3' end
Watson and Crick model of DNA secondary structure - Ans:✔✔-has exterior sugar-phosphate backbone
with bases facing each other in the interior
Bases of two strands bond Via H bonds
There are hydrophobic interactions between bases of adjacent nucleotides
Strands are antiparallel
Base Bonds - Ans:✔✔-G-C triple bond
A-T double bond
Three Hypothesis for DNA replication - Ans:✔✔-semiconservative replication (right)
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, ©GRACEAMELIA 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED
FIRST PUBLISH OCTOBER 2024
conservative replication
dispersive replication
DNA replication - Ans:✔✔-DNA polymerase Catalyzes DNA synthesis
DNA polymerase requires an OH group to bind to
DNA grows on the 3' end
Origin of replication is where the replication starts
Usually more A-T at origin
dNTP deoxyribonucleoside triphosphate - Ans:✔✔-precursor for DNucleotides
Types of DNA polymerase - Ans:✔✔-DNA polymerase 1 and 3 complete DNA replication
DNA polymerase 2 repairs DNA
Helicase - Ans:✔✔-Breaks H bonds between DNA strands
SSBPs (single strand DNA binding proteins) - Ans:✔✔-stabalizes single strands of DNA after bonds are
broken
Keeps the strands seperate
Topoisomerase - Ans:✔✔-cuts and rejoins DNA downstream to relieve tension force
Page 3/29
FIRST PUBLISH OCTOBER 2024
Biology 116 Final Exam Questions and
Answers
Determination of what DNA is made of - Ans:✔✔-Hershey and Chase marked Virus's with two different
radioactive molecules (32p labels DNA, 35s labels protein)
32p was found only inside of infected cells
35s was found in ghost shells
Nucleic Acid - Ans:✔✔-polymer of nucleotide monomer
Nucleotide - Ans:✔✔-phosphate group+ sugar+ base
synthesized from nucleoside triphosphates (NTPs)
Nucleoside - Ans:✔✔-sugar + base
Ribose - Ans:✔✔-found in RNA
contains additional O on 2' end
Deoxyribose - Ans:✔✔-found in DNA
Page 1/29
, ©GRACEAMELIA 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED
FIRST PUBLISH OCTOBER 2024
no O on 2' end
Purines - Ans:✔✔-Guanine and Adenine (double ring)
Pyrimidines - Ans:✔✔-Cytosine Uracil and Thymine (single ring)
Polymerization of of Nucleotides - Ans:✔✔-3' hydroxyl group in the sugar links with the phosphate group
of another nucleotide
Dehydration reaction
Polarity of Nucleic acids - Ans:✔✔-has polarity extending from free 5' to 3' end
Watson and Crick model of DNA secondary structure - Ans:✔✔-has exterior sugar-phosphate backbone
with bases facing each other in the interior
Bases of two strands bond Via H bonds
There are hydrophobic interactions between bases of adjacent nucleotides
Strands are antiparallel
Base Bonds - Ans:✔✔-G-C triple bond
A-T double bond
Three Hypothesis for DNA replication - Ans:✔✔-semiconservative replication (right)
Page 2/29
, ©GRACEAMELIA 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED
FIRST PUBLISH OCTOBER 2024
conservative replication
dispersive replication
DNA replication - Ans:✔✔-DNA polymerase Catalyzes DNA synthesis
DNA polymerase requires an OH group to bind to
DNA grows on the 3' end
Origin of replication is where the replication starts
Usually more A-T at origin
dNTP deoxyribonucleoside triphosphate - Ans:✔✔-precursor for DNucleotides
Types of DNA polymerase - Ans:✔✔-DNA polymerase 1 and 3 complete DNA replication
DNA polymerase 2 repairs DNA
Helicase - Ans:✔✔-Breaks H bonds between DNA strands
SSBPs (single strand DNA binding proteins) - Ans:✔✔-stabalizes single strands of DNA after bonds are
broken
Keeps the strands seperate
Topoisomerase - Ans:✔✔-cuts and rejoins DNA downstream to relieve tension force
Page 3/29
