FIRST PUBLISH OCTOBER 2024
NURS 236 - Midterm 2 Exam Questions
and Answers
Sterilization - Ans:✔✔-removing and destroying all MOs (including endospores)
Example: Cleaning of surgical instruments
Disinfection - Ans:✔✔-treatment that kills or inhibits the growth of harmful MOS
Example: cleaning a hospital room between patients
Sanitization - Ans:✔✔-reduction of microbial contamination to levels safe by public health standards
Example: food preparation areas
Antisepsis - Ans:✔✔-disinfection of living sample, such as skin
Example: showering before a surgery
Degerming - Ans:✔✔-antisepsis of a limited area
Example: cleaning skin before IV insertion
Asepsis - Ans:✔✔-absence of significant contamination
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Biocide/germicide - Ans:✔✔-treatments that kill living cells
Bacteriostasis - Ans:✔✔-treatment that halts or prevents continued growth of bacteria
Which form of microbial control is the most powerful? - Ans:✔✔-Sterilization
Which form of microbial control is the least powerful? - Ans:✔✔-Bacteriostasis
opportunistic infection - Ans:✔✔-an infection that causes disease when the immune system is
compromised for other reasons
What are the 4 control agent activities? - Ans:✔✔-physical/chemical, plasma membrane, proteins,
nucleic acids
Physical Methods - Boiling water - Ans:✔✔-Influenced by air pressure
Kills MOs in 10min
Hepatitis in 30min
Endospores up to 2h
Physical Methods - Autoclaving - Ans:✔✔-Uses pressurized steam to get high temps, requires exposure
to move steam
Kills endospores in 15min
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, ©GRACEAMELIA 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED
FIRST PUBLISH OCTOBER 2024
Cannot kill prion
Physical Methods - Moist Heat - Ans:✔✔-pasteurization
Physical Methods - Dry Heat - Ans:✔✔-flaming, incineration, hot air sterilization
Physical Methods - Low Heat - Ans:✔✔-depends on what is being sterilized.
4C - -20C
Physical Methods - Filtration - Ans:✔✔-physical separation of of MOs out of a sample based on size
Only liquids or gases
Health environments use HEPA (high efficiency particulate air) Filters
Membrane sterilization of liquids --> heat sensitive
Physical Methods - Dessication - Ans:✔✔-removing water needed for survival, many go dormant
the state of extreme dryness
Neissaria gonorrhae - 1h
Mycobacterium tuberculosis - months
Endospores - centuries
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