Microscopy
* resolution the
ability to individual
objects separate entities
-
see as
size
image
I
magnification calculation : -Image
size
actualsin ATM
a
magnifica
melve : Im
im : Mo Electron Microsy
um : 1x106 i
nm (x109
:
* in
light microscopy magnification ,
can be increased easily however resolution is
a
limiting factor
teelection microscopy <Inm is used to illuminate the species detail of the cell
- beam
of electing / a -
ultrastructure/beceen as the h elections : a tran
light
Disadvantages :
↳ expensive
↳ must be trained to use it
Le can
only be used in a specic environment
↳ specimen could be damaged electron beam
by
↳ specimen cannot be alive - must be placed in a vacuum before. : must
already be dead
↑ ransmission electron microcaps (TEM) :
↳ beam
of elections are transmitted tre a
specimen + focussed to produce an
image ->
best resolution
↳ electrons pass turn contrast
dense parts deasily creating a
↳ nat image = 20
↳ possible magnification up to X2 million
↳ resolving power O Sum
.
&
Scanning Electron Micnecope (SEM)
minWe e
↳ elections are collected it
beam
of elections are sent across the surface
of a specimen
and reflected
↳ resolving power 3-10 mm so resolution not TEM
as good as the
↳ elems bounced
if sample
↳ final image 3D
↳ possible magnification is x200 , 00
Laser Scanning Confocal microscopy :
An light intensity is used to illuminate a specimen which has been treated w/ florescent de
↳ final
image is coloured
* can use tuck samples/while organisms
↳ the Laser is reflected by the dyes
* ↑ resolchen
used
*
by medics as it is a non-invasive method
↓ than election
* expensive
* cmage 3D
final
* resolution the
ability to individual
objects separate entities
-
see as
size
image
I
magnification calculation : -Image
size
actualsin ATM
a
magnifica
melve : Im
im : Mo Electron Microsy
um : 1x106 i
nm (x109
:
* in
light microscopy magnification ,
can be increased easily however resolution is
a
limiting factor
teelection microscopy <Inm is used to illuminate the species detail of the cell
- beam
of electing / a -
ultrastructure/beceen as the h elections : a tran
light
Disadvantages :
↳ expensive
↳ must be trained to use it
Le can
only be used in a specic environment
↳ specimen could be damaged electron beam
by
↳ specimen cannot be alive - must be placed in a vacuum before. : must
already be dead
↑ ransmission electron microcaps (TEM) :
↳ beam
of elections are transmitted tre a
specimen + focussed to produce an
image ->
best resolution
↳ electrons pass turn contrast
dense parts deasily creating a
↳ nat image = 20
↳ possible magnification up to X2 million
↳ resolving power O Sum
.
&
Scanning Electron Micnecope (SEM)
minWe e
↳ elections are collected it
beam
of elections are sent across the surface
of a specimen
and reflected
↳ resolving power 3-10 mm so resolution not TEM
as good as the
↳ elems bounced
if sample
↳ final image 3D
↳ possible magnification is x200 , 00
Laser Scanning Confocal microscopy :
An light intensity is used to illuminate a specimen which has been treated w/ florescent de
↳ final
image is coloured
* can use tuck samples/while organisms
↳ the Laser is reflected by the dyes
* ↑ resolchen
used
*
by medics as it is a non-invasive method
↓ than election
* expensive
* cmage 3D
final
