ASCP HT EXAM
What are the color results of the Warthin-Starry technique?
Churukian & Schenk modification of the Warthin-Starry technique
What stain is demonstrated in the photo?
Alipia felis - Black
Legionella pnuemophila - Black
N asteroides - Black
H pylori - Black
Nuclei - Brown
Erythrocytes - Brown
What are the color results of the Churukian & Schenk modification of the
Warthin-Starry technique
Dieterle method
What stain is demonstrated in the photo?
To demonstrate spirochetes or the causative organism of legionellosis
What is the purpose of the Dieterle method?
Alcoholic uranyl nitrate 5% (Uranyl nitrate and alcohol 70%), Silver nitrate 1%,
Developer (Hydroquinone, Sodium sulfite, DI water, Acetone, Formaldehyde,
Pyridine, and Alcoholic gum mastic), and Formic acid 10%
What are the reagents used in the Dieterle method?
Spirochetes, bacteria - Brown to black
,Background - Pale yellow or tan
What are the color results of the Dieterle method?
Steiner and steiner procedure for spirochetes, H.pylori, and legionella.
What is the stain demonstrated in the photo?
The demonstration of spirochetes, H.pylori, and the causative organism of
legionellosis
What is the purpose of the Steiner and steiner procedure?
Uranyl nitrate 1%, Silver nitrate 1%, Silver nitrate 0.04%, Reducing solution (Gum
mastic, Hydroquinone, and Absolute alcohol)
What are the reagents used in the Steiner and steiner procedure?
Spirochetes, H.Pylori, L pneumophila, and other nonfilamentous bacteria - Dark
brown to Black
Background - Light Yellow
What are the color results of the Steiner and steiner procedure?
Lipofuscin and ceroid
What are the lipidic nonhomogeneous pigments?
melanin
What is the nonlipidic nonhomogeneous pigment?
Iron - hemosiderin
Bile pigments - 1) Biliverdin or biliverdin broken down into 2) Bilirubin
What are the homogeneous pigments?
Carbon - anthracotic pigment
Asbestos - Bifringent
,Tattoo pigment
Copper (Wilsons disease)
What are the most common exogenous pigments?
Urate crystals (Gouty tophi) - Bifringent
What is the most common endogenous deposit?
Calcium
Ferrous and ferric iron
Cupric
Phosphate
Carbonate
What are some common minerals demonstrated in pigment stains?
Prussian Blue for ferric iron
What stain is demonstrated in the photo?
To detect ferric iron in tissues and aid in the diagnosis of idiopathic
hemochromatosis.
What is the purpose of the Prussian blue stain?
Loosely bound protein complexes (such as hemosiderin) but not strongly bound
like in hemoglobin.
What type of protein complexes does the Prussian blue stain detect ferric iron in?
Potassium ferrrocyanide 2%, HCL 2%, and Nuclear fast red.
What reagents are used in the Prussian blue stain?
Nuclei and hemofuchsin - Bright red
, Hemosiderin - Blue
Background - Pink
What are the color results of the Prussian Blue stain?
Turnbull blue for ferrous iron
What stain is demonstrated in the photo?
To detect of ferrous iron in tissue.
What is the purpose of the Turnbull blue stain?
Ferrous Ferricyanide
What is another name for the Turnbull blue pigment?
0.06N HCL, Potassium ferricyanide, Acetic acid 1%, and nuclear fast red.
What are the reagents used in the Turnbull blue stain?
Ferrous iron - Blue
Background - Pink-Red
What are the color results of the Turnbull blue stain?
Schmorl technique
What is the stain demonstrated in the photo?
To indicate the presence of reducing substances in tissue. Will stain argentaffin
granules, formalin pigment, and melanin
What is the purpose of the Schmorl technique?
Turnbull Blue
What is the final pigment formed by the Schmorl technique?
What are the color results of the Warthin-Starry technique?
Churukian & Schenk modification of the Warthin-Starry technique
What stain is demonstrated in the photo?
Alipia felis - Black
Legionella pnuemophila - Black
N asteroides - Black
H pylori - Black
Nuclei - Brown
Erythrocytes - Brown
What are the color results of the Churukian & Schenk modification of the
Warthin-Starry technique
Dieterle method
What stain is demonstrated in the photo?
To demonstrate spirochetes or the causative organism of legionellosis
What is the purpose of the Dieterle method?
Alcoholic uranyl nitrate 5% (Uranyl nitrate and alcohol 70%), Silver nitrate 1%,
Developer (Hydroquinone, Sodium sulfite, DI water, Acetone, Formaldehyde,
Pyridine, and Alcoholic gum mastic), and Formic acid 10%
What are the reagents used in the Dieterle method?
Spirochetes, bacteria - Brown to black
,Background - Pale yellow or tan
What are the color results of the Dieterle method?
Steiner and steiner procedure for spirochetes, H.pylori, and legionella.
What is the stain demonstrated in the photo?
The demonstration of spirochetes, H.pylori, and the causative organism of
legionellosis
What is the purpose of the Steiner and steiner procedure?
Uranyl nitrate 1%, Silver nitrate 1%, Silver nitrate 0.04%, Reducing solution (Gum
mastic, Hydroquinone, and Absolute alcohol)
What are the reagents used in the Steiner and steiner procedure?
Spirochetes, H.Pylori, L pneumophila, and other nonfilamentous bacteria - Dark
brown to Black
Background - Light Yellow
What are the color results of the Steiner and steiner procedure?
Lipofuscin and ceroid
What are the lipidic nonhomogeneous pigments?
melanin
What is the nonlipidic nonhomogeneous pigment?
Iron - hemosiderin
Bile pigments - 1) Biliverdin or biliverdin broken down into 2) Bilirubin
What are the homogeneous pigments?
Carbon - anthracotic pigment
Asbestos - Bifringent
,Tattoo pigment
Copper (Wilsons disease)
What are the most common exogenous pigments?
Urate crystals (Gouty tophi) - Bifringent
What is the most common endogenous deposit?
Calcium
Ferrous and ferric iron
Cupric
Phosphate
Carbonate
What are some common minerals demonstrated in pigment stains?
Prussian Blue for ferric iron
What stain is demonstrated in the photo?
To detect ferric iron in tissues and aid in the diagnosis of idiopathic
hemochromatosis.
What is the purpose of the Prussian blue stain?
Loosely bound protein complexes (such as hemosiderin) but not strongly bound
like in hemoglobin.
What type of protein complexes does the Prussian blue stain detect ferric iron in?
Potassium ferrrocyanide 2%, HCL 2%, and Nuclear fast red.
What reagents are used in the Prussian blue stain?
Nuclei and hemofuchsin - Bright red
, Hemosiderin - Blue
Background - Pink
What are the color results of the Prussian Blue stain?
Turnbull blue for ferrous iron
What stain is demonstrated in the photo?
To detect of ferrous iron in tissue.
What is the purpose of the Turnbull blue stain?
Ferrous Ferricyanide
What is another name for the Turnbull blue pigment?
0.06N HCL, Potassium ferricyanide, Acetic acid 1%, and nuclear fast red.
What are the reagents used in the Turnbull blue stain?
Ferrous iron - Blue
Background - Pink-Red
What are the color results of the Turnbull blue stain?
Schmorl technique
What is the stain demonstrated in the photo?
To indicate the presence of reducing substances in tissue. Will stain argentaffin
granules, formalin pigment, and melanin
What is the purpose of the Schmorl technique?
Turnbull Blue
What is the final pigment formed by the Schmorl technique?
