BIOCHEMISTRY LAB REPORT 4 2023/2024|
PORTAGE LEARNING|WITH PROCEDURES,
EXPLANATION AND FINAL RESULTS.HIGHLY
VERIFIRD Experiment #: Lab 4 Title: Chromatography
of molecules.
Which is the correct definition of photosynthesis - ANSWER the process used by
organisms to convert light energy to chemical energy
where does the light reaction of photosynthesis take place - ANSWER in the
thylakoid membrane
where is the light energy converted into an electron flow - ANSWER in the reaction
center chlorophyll
in what order do the electrons move through the electron transport chain - ANSWER
water - photosystem 2 - photosystem 1 - NADPH
chemical equation? - ANSWER 2 H20 + 2NADP+ + nADP + nPi + hv —> ........
where does the electron flow that we measure with DCPIP originate - ANSWER
photosystem 2
what would be a good positive control for this experiment - ANSWER expose a
sample to visible light
what would be the best negative control - ANSWER keep a sample in the dark
what wavelength - ANSWER 580 nm
hypothesis - ANSWER yes
the sample in the spectrophotometer will have - ANSWER less oxidized DCPIP than
the negative control
why do we use DCMU - ANSWER we avoid further reduction of DCPIP under the lab
light
what wavelength for oxidized DCPIP (blue) - ANSWER 610 nm
is the red pigment really a photosynthetic pigment - ANSWER Yes, because
photosystem 2 was active in green light
PORTAGE LEARNING|WITH PROCEDURES,
EXPLANATION AND FINAL RESULTS.HIGHLY
VERIFIRD Experiment #: Lab 4 Title: Chromatography
of molecules.
Which is the correct definition of photosynthesis - ANSWER the process used by
organisms to convert light energy to chemical energy
where does the light reaction of photosynthesis take place - ANSWER in the
thylakoid membrane
where is the light energy converted into an electron flow - ANSWER in the reaction
center chlorophyll
in what order do the electrons move through the electron transport chain - ANSWER
water - photosystem 2 - photosystem 1 - NADPH
chemical equation? - ANSWER 2 H20 + 2NADP+ + nADP + nPi + hv —> ........
where does the electron flow that we measure with DCPIP originate - ANSWER
photosystem 2
what would be a good positive control for this experiment - ANSWER expose a
sample to visible light
what would be the best negative control - ANSWER keep a sample in the dark
what wavelength - ANSWER 580 nm
hypothesis - ANSWER yes
the sample in the spectrophotometer will have - ANSWER less oxidized DCPIP than
the negative control
why do we use DCMU - ANSWER we avoid further reduction of DCPIP under the lab
light
what wavelength for oxidized DCPIP (blue) - ANSWER 610 nm
is the red pigment really a photosynthetic pigment - ANSWER Yes, because
photosystem 2 was active in green light
