BIOCHEMISTRY FINAL EXAM
QUESTIONS AND THEIR
RESPECTIVE ANSWERS
It is important for cells to degrade misfolded proteins.
If misfolded proteins are not degraded, the misfolded
proteins may
aggregate and interfere with normal cellular function
Which form(s) of DNA exhibit(s) a zigzag
arrangement?
Z-DNA
In DNA, the phosphate groups are on the outside of
the helix. Why does this stabilize the structure?
Ionic interactions with the solvent
A segment of DNA containing 20 base pairs includes 8
adenine residues. How many uracil residues are
present?
0
The difference between an oxidation reaction and a
reduction reaction is that oxidation is the _______ and
reduction is the __________.
Loss of electrons; gain of electrons
As a protein folds, what are the stabilizing factors that
help keep the protein folded?
Hydrophobic amino acids on the interior and hydrophilic
amino acids on the exterior
The amino acid sequence of a protein determines its
structure. Which of the following statements is true?
,Two proteins with similar amino acid sequence should
have similar structures.
Which of the following expresses the relationship
among the linking number, twist and writhe?
Lk = Tw + Wr
The interior stacking of the DNA bases in the double
helix provides stability through
Hydrophobic and van der Waals interactions
Which of the following best defines the first law of
thermodynamics
total amount of energy in the universe is constant
If an unknown solution has low pKa value, it can be
said with certainty that it is
a strong acid
In multi-subunit proteins, such as hemoglobin, the
different subunits are usually bound to one
another by all of the following EXCEPT
peptide bonds
Simple sugars are made of which of the following
elements
C, O and H
You wish to prepare a solution with a pH of 5.44. If the
pKa of the weak acid is 4.74, what ratio of weak base
to weak acid should you use?
5.0
Which of the following statements about the chamber-
type chaperone protein GroEL-GroES is correct?
Gro-EL-Gro-ES requires the hydrolysis of multiple ATPs to
assist in the folding of a protein
Two dimensional polyacryolamide gel electrophoresis
separates proteins based on
, pI and molecular weight
Which of the following statements about
Ramachandran plots is true?
They show equal distribution of two angles with weird
symbols for a-helical and b-sheet containing protein
Which separation technique exploits the solubility
differences of proteins
Salting out
Which stabilizing force in protein tertiary structures is
a covalent bonding force?
Disulfide bonding
Why can a guanine not be paired with adenine
Guanine can form three hydrogen bonds and adenine can
form two
A hydrogen bond can best be described as a
weak noncovalent interaction.
Enzymes function as reaction catalysts in cells. If the
enzymes were removed from a cell, the rate of
biochemical reactions would
decrease
Type II topoisomerase enzymes are important in
replication and transcription because they
relieve the positive supercoiling
Even though amylose and cellulose contain the same
repeating unit of glucose, they are very different in
terms of function. Why?
The orientations of the glycosidic bond are different
The specific sites on the antigen that interacts with
the antibody are called
epitopes
QUESTIONS AND THEIR
RESPECTIVE ANSWERS
It is important for cells to degrade misfolded proteins.
If misfolded proteins are not degraded, the misfolded
proteins may
aggregate and interfere with normal cellular function
Which form(s) of DNA exhibit(s) a zigzag
arrangement?
Z-DNA
In DNA, the phosphate groups are on the outside of
the helix. Why does this stabilize the structure?
Ionic interactions with the solvent
A segment of DNA containing 20 base pairs includes 8
adenine residues. How many uracil residues are
present?
0
The difference between an oxidation reaction and a
reduction reaction is that oxidation is the _______ and
reduction is the __________.
Loss of electrons; gain of electrons
As a protein folds, what are the stabilizing factors that
help keep the protein folded?
Hydrophobic amino acids on the interior and hydrophilic
amino acids on the exterior
The amino acid sequence of a protein determines its
structure. Which of the following statements is true?
,Two proteins with similar amino acid sequence should
have similar structures.
Which of the following expresses the relationship
among the linking number, twist and writhe?
Lk = Tw + Wr
The interior stacking of the DNA bases in the double
helix provides stability through
Hydrophobic and van der Waals interactions
Which of the following best defines the first law of
thermodynamics
total amount of energy in the universe is constant
If an unknown solution has low pKa value, it can be
said with certainty that it is
a strong acid
In multi-subunit proteins, such as hemoglobin, the
different subunits are usually bound to one
another by all of the following EXCEPT
peptide bonds
Simple sugars are made of which of the following
elements
C, O and H
You wish to prepare a solution with a pH of 5.44. If the
pKa of the weak acid is 4.74, what ratio of weak base
to weak acid should you use?
5.0
Which of the following statements about the chamber-
type chaperone protein GroEL-GroES is correct?
Gro-EL-Gro-ES requires the hydrolysis of multiple ATPs to
assist in the folding of a protein
Two dimensional polyacryolamide gel electrophoresis
separates proteins based on
, pI and molecular weight
Which of the following statements about
Ramachandran plots is true?
They show equal distribution of two angles with weird
symbols for a-helical and b-sheet containing protein
Which separation technique exploits the solubility
differences of proteins
Salting out
Which stabilizing force in protein tertiary structures is
a covalent bonding force?
Disulfide bonding
Why can a guanine not be paired with adenine
Guanine can form three hydrogen bonds and adenine can
form two
A hydrogen bond can best be described as a
weak noncovalent interaction.
Enzymes function as reaction catalysts in cells. If the
enzymes were removed from a cell, the rate of
biochemical reactions would
decrease
Type II topoisomerase enzymes are important in
replication and transcription because they
relieve the positive supercoiling
Even though amylose and cellulose contain the same
repeating unit of glucose, they are very different in
terms of function. Why?
The orientations of the glycosidic bond are different
The specific sites on the antigen that interacts with
the antibody are called
epitopes
