BI 234 LIGHT MICROSCOPES EXAM QUESTIONS AND
ANSWERS WITH COMPLETE SOLUTIONS VERIFIED
bright field microscopy
To observe killed
stained specimens
and naturally colored
live ones; also used to
count microorganisms
dark field microscopy
a special condenser is used so only the light reflected off the specimen enters the
objective.
specimen against a dark background.
To observe living,
colorless, unstained
Phase-contrast
transforms subtle changes in light waves passing through the specimen into differences
in light intensity, best for observing intracellular structures
Uses a special condenser that splits a
polarized light beam into
, two beams: one passes through the specimen, and
one of which bypasses the specimen; the
beams are then rejoined before entering
the oculars; contrast in the image results
from the interactions of the two beams
Living Things.
Differential interference
contrast (Nomarski)
Fluorescence
uses UV light
Confocal
Used UV lasers.
Transmission Electron Microscope (TEM)
What are the 5 a sexual methods prolaryotes used for reproduction?
Budding,
binary fission (most common),
snapping division
Spores
Viviparity
binary fission
Replicates chromosomes, elongates,splits off into two daughter cells. Identical genetic
make up as parents. Very quick process.
ANSWERS WITH COMPLETE SOLUTIONS VERIFIED
bright field microscopy
To observe killed
stained specimens
and naturally colored
live ones; also used to
count microorganisms
dark field microscopy
a special condenser is used so only the light reflected off the specimen enters the
objective.
specimen against a dark background.
To observe living,
colorless, unstained
Phase-contrast
transforms subtle changes in light waves passing through the specimen into differences
in light intensity, best for observing intracellular structures
Uses a special condenser that splits a
polarized light beam into
, two beams: one passes through the specimen, and
one of which bypasses the specimen; the
beams are then rejoined before entering
the oculars; contrast in the image results
from the interactions of the two beams
Living Things.
Differential interference
contrast (Nomarski)
Fluorescence
uses UV light
Confocal
Used UV lasers.
Transmission Electron Microscope (TEM)
What are the 5 a sexual methods prolaryotes used for reproduction?
Budding,
binary fission (most common),
snapping division
Spores
Viviparity
binary fission
Replicates chromosomes, elongates,splits off into two daughter cells. Identical genetic
make up as parents. Very quick process.
