BI 234 LAB FINAL PCC AYALA EXAM ACTUAL QUESTIONS AND
ANSWERS WITH COMPLETE VERIFIED SOLUTIONS
bright field microscopy
most commonly used in lab, observe live or preserved specimens
fluorescent microscopy
fluorescence stain/dye, diagnostic tool
Electron Microscope (TEM) (SEM)
magnifies objects 10,000x-100,000x provides detailed views of bacteria, viruses,
internal cellular structures
Inoculation
Sample placed into sterile medium w/ nutrients to support growth
Incubation
maintaining something at the most favorable temperature for its development
Isolation
isolation of microbe in macroscopic form (colony)
Inspection
macroscopic observation and slide preparation for microscopic observation
Identification
determine type of microbe, via observation, examination, staining
Physical properties of media
Liquid, semi-solid, solid
, Liquid Media properties
water based solutions, aka broths, milks, infusions
Semi-solid media
low percentage <1% of agar, used for motility testing
Solid media
high percentage of agar 1-5%, enables formation of discrete colonies
Chemical Content of Media
Synthetic, Nonsynthetic/complex
Synthetic Media
contains pure organic and inorganic compounds in an exact chemical formula
complex media
contain some ingredients of unknown composition and/or concentration
functional media
Enriched media, selective media, differential media
Agar
solidifying agent, not nutritive, allows for growth on plates
serological pipette
pipettes that have graduations down to the tip. They are temperature
calibrated which means that the volume markings on the side of the pipette are accurate
within a
certain temperature range usually 20-25oC (68-77oF).
Disposable transfer pipette
disposable pipette
ANSWERS WITH COMPLETE VERIFIED SOLUTIONS
bright field microscopy
most commonly used in lab, observe live or preserved specimens
fluorescent microscopy
fluorescence stain/dye, diagnostic tool
Electron Microscope (TEM) (SEM)
magnifies objects 10,000x-100,000x provides detailed views of bacteria, viruses,
internal cellular structures
Inoculation
Sample placed into sterile medium w/ nutrients to support growth
Incubation
maintaining something at the most favorable temperature for its development
Isolation
isolation of microbe in macroscopic form (colony)
Inspection
macroscopic observation and slide preparation for microscopic observation
Identification
determine type of microbe, via observation, examination, staining
Physical properties of media
Liquid, semi-solid, solid
, Liquid Media properties
water based solutions, aka broths, milks, infusions
Semi-solid media
low percentage <1% of agar, used for motility testing
Solid media
high percentage of agar 1-5%, enables formation of discrete colonies
Chemical Content of Media
Synthetic, Nonsynthetic/complex
Synthetic Media
contains pure organic and inorganic compounds in an exact chemical formula
complex media
contain some ingredients of unknown composition and/or concentration
functional media
Enriched media, selective media, differential media
Agar
solidifying agent, not nutritive, allows for growth on plates
serological pipette
pipettes that have graduations down to the tip. They are temperature
calibrated which means that the volume markings on the side of the pipette are accurate
within a
certain temperature range usually 20-25oC (68-77oF).
Disposable transfer pipette
disposable pipette
