BI 234: LAB (WEEK 1/2) EXAM QUESTIONS WITH
VERIFIED CORRECT ANSWERS LATEST UPDATE
Contrast
Difference in intensity between two objects, or an object and background
Magnification
Increase in size of apparent image
Resolution
Ability to distinguish two entities as separate
What is the difference between a simple and a compound light microscope?
A simple light microscope uses one magnifying lens meanwhile compound light
microscope uses a series of lens for magnification
Name two ways to adjust the light reaching the specimen
Light intensity knob and iris diaphragm
What adjustment can you make to increase contrast? (other than staining)
Condenser, iris diaphragm??
Describe the process of cleaning and storing the microscope.
Set stage to the lowest, set lens to 4x, set light intensity to the lowest, turn off, wrap
cord
All oil must be removed, use lens paper only to clean the lenses, begin with 4x and
move up
KimWipes may be used to clean the objective lenses of a compound microscope.
, F, use lens paper only
The coarse adjustment should be used first when examining a specimen with the
oil immersion objective before using the fine focus.
F, use fine focus only with oil immersion
It is OK to go back and for the between the 40x and 100x objective when
observing a specimen
F, oil should not get on any other lens except oil immersion
Used petri dishes should go in normal trash
F, go in the biohazard bucket
It is OK to go back and for the between the 40x and 100x objective when
observing a specimen
F, oil should not get on any other lens except oil immersion
Test tubes used to grow bacteria should go in sharps biohazard disposal
F, return to racks
Low dry, total magnification
100x
Oil immersion lens, total magnification
1000x
High dry, total magnification
400x
Scanning objective, total magnification
40x
VERIFIED CORRECT ANSWERS LATEST UPDATE
Contrast
Difference in intensity between two objects, or an object and background
Magnification
Increase in size of apparent image
Resolution
Ability to distinguish two entities as separate
What is the difference between a simple and a compound light microscope?
A simple light microscope uses one magnifying lens meanwhile compound light
microscope uses a series of lens for magnification
Name two ways to adjust the light reaching the specimen
Light intensity knob and iris diaphragm
What adjustment can you make to increase contrast? (other than staining)
Condenser, iris diaphragm??
Describe the process of cleaning and storing the microscope.
Set stage to the lowest, set lens to 4x, set light intensity to the lowest, turn off, wrap
cord
All oil must be removed, use lens paper only to clean the lenses, begin with 4x and
move up
KimWipes may be used to clean the objective lenses of a compound microscope.
, F, use lens paper only
The coarse adjustment should be used first when examining a specimen with the
oil immersion objective before using the fine focus.
F, use fine focus only with oil immersion
It is OK to go back and for the between the 40x and 100x objective when
observing a specimen
F, oil should not get on any other lens except oil immersion
Used petri dishes should go in normal trash
F, go in the biohazard bucket
It is OK to go back and for the between the 40x and 100x objective when
observing a specimen
F, oil should not get on any other lens except oil immersion
Test tubes used to grow bacteria should go in sharps biohazard disposal
F, return to racks
Low dry, total magnification
100x
Oil immersion lens, total magnification
1000x
High dry, total magnification
400x
Scanning objective, total magnification
40x
