BI 234 CULTURING METHODS (EXAM 1) QUESTIONS AND
ANSWERS WITH COMPLETE SOLUTIONS VERIFIED
Five I's
I. inoculation
II. incubation
III. isolation
IV. inspection
V. identification
inoculation
producing a culture
medium
nutrient substance used for microbial growth
broth
a liquid medium that allows growth throughout
agar
gelatinous, yet firm medium, isolated from algae
streak plate
spreading microorganisms across the surface of agar
pure culture
, population of cloned cells descended from a single cell, growing in a laboratory medium
population
organisms living in a given area
Chemically defined (synthetic) media
contains an exact, known chemical composition
Complex media
exact chemical composition is not known or unimportant
Reducing media
uses chemicals to lower the O2 concentrations, used to grow anaerobic bacteria
Selective media
encourages the growth of desired organism while suppressing the growth of unwanted bacteria
Differential media
accentuates species differences by displaying visible differences in colony color or media color change
Enrichment media
used to increase small numbers of desired microbes to detectable levels; target microbe is provided with
special nutrients to encourage growth
Incubation
optimizing bacterial growth
bacterial growth
ANSWERS WITH COMPLETE SOLUTIONS VERIFIED
Five I's
I. inoculation
II. incubation
III. isolation
IV. inspection
V. identification
inoculation
producing a culture
medium
nutrient substance used for microbial growth
broth
a liquid medium that allows growth throughout
agar
gelatinous, yet firm medium, isolated from algae
streak plate
spreading microorganisms across the surface of agar
pure culture
, population of cloned cells descended from a single cell, growing in a laboratory medium
population
organisms living in a given area
Chemically defined (synthetic) media
contains an exact, known chemical composition
Complex media
exact chemical composition is not known or unimportant
Reducing media
uses chemicals to lower the O2 concentrations, used to grow anaerobic bacteria
Selective media
encourages the growth of desired organism while suppressing the growth of unwanted bacteria
Differential media
accentuates species differences by displaying visible differences in colony color or media color change
Enrichment media
used to increase small numbers of desired microbes to detectable levels; target microbe is provided with
special nutrients to encourage growth
Incubation
optimizing bacterial growth
bacterial growth
