BIOS 136 Purification
Goal of purification - answersensure you end up with a phage sample that contains only
one types of phage
Phage particles that are genetic clones - answershomogenous or clonal population
Biological properties used to categorize phage - answersmorphology, plaque size and
genome sequence
How is purification accomplished? - answersSeparating the phage by diluting the
samples so that only a few phage particles are place don a plate
What are the consequences of phage particles diffusing within agar plate? -
answersphage particles from a nearby plaque could diffuse into your selected plaque
If multiple spot test are present... - answersyou should not pick from a spot plate
What should you use when you perform phage purification? - answersoriginal direct
isolation plaque or enriched culture
What types of plate do you collect a lysate from? - answersplates densely packed with
plaques, webbed plates
What is titer calculated from? - answersthe number of plaques formed from a given
volume of and dilution of your lysate
What does a spot titer provide? - answersa quick titer estimate, often inaccurate and
provides only an estimate, allows you to predict dilutions will result in countable number
of plaques on a full plate titer
Units for titer - answerspfu/mL (plaque-forming units in every milliliter of lysate)
Goal of purification - answersensure you end up with a phage sample that contains only
one types of phage
Phage particles that are genetic clones - answershomogenous or clonal population
Biological properties used to categorize phage - answersmorphology, plaque size and
genome sequence
How is purification accomplished? - answersSeparating the phage by diluting the
samples so that only a few phage particles are place don a plate
What are the consequences of phage particles diffusing within agar plate? -
answersphage particles from a nearby plaque could diffuse into your selected plaque
If multiple spot test are present... - answersyou should not pick from a spot plate
What should you use when you perform phage purification? - answersoriginal direct
isolation plaque or enriched culture
What types of plate do you collect a lysate from? - answersplates densely packed with
plaques, webbed plates
What is titer calculated from? - answersthe number of plaques formed from a given
volume of and dilution of your lysate
What does a spot titer provide? - answersa quick titer estimate, often inaccurate and
provides only an estimate, allows you to predict dilutions will result in countable number
of plaques on a full plate titer
Units for titer - answerspfu/mL (plaque-forming units in every milliliter of lysate)
