MCB 2010C Microbiology Final Exam Latest 2024|2025
FALL-SPRING FSW GRADED A+
If the 10x objective is used, what is the total magnification of the slide? (Assume the
oculars have a magnification of 10x.) - ANSWER: 100x
Which objective should be used with immersion oil? - ANSWER: The 100x objective
Why is the 40x objective cleaned before the 100x oil immersion objective? -
ANSWER: To prevent the transfer of immersion oil from the 100x to the 40x
objective
Using a microscope with 10x oculars, the total magnification of a specimen with a 4x
objective will be - ANSWER: 40x
A laboratory photo atlas contains an image with a total magnification listed as 1000x.
Using a microscope with 10x ocular magnification, which objective should be used to
recreate the image? - ANSWER: 100x objective
With which objective can you use both the course and fine focus controls? -
ANSWER: The 4x objective
Immersion oil is used with - ANSWER: objectives with magnifications of 50x or
greater
When cleaning the microscope, the ________ objective should be cleaned prior to
the _______ objective to prevent the transfer of oil between them. - ANSWER:
40x;100x
Why is it challenging to focus on bacteria in a hanging drop preparation? - ANSWER:
The bacteria in a hanging drop preparation are not stained.
Nitrogen fixation is the - ANSWER: conversion of atmospheric nitrogen to organic
forms
Chloroplasts are used to carry out ________ by algae. - ANSWER: photosynthesis
Algae are ________ and cyanobacteria are - ANSWER: eukaryotic; prokaryotic
What is important to remember when using oil immersion? - ANSWER: Immersion oil
can only be used with the 100X objective, and without it, the image is blurry.
What are advantages to using a wet mount slide preparation?Select all that apply -
ANSWER: A. There are additional advantages to viewing a living specimen.
C. There are additional advantages to viewing a motile specimen.
, D. Wet mount slide preparation allows the viewing of specimen behavior as they
interact with the environment.
Which of the three factors affecting image quality is altered by the light source? -
ANSWER: Contrast
How do the coarse and fine focus knobs work on a brightfield microscope? -
ANSWER: The focus knobs move the stage up and down
Why should the fine focus knob typically be used with the 10X and 40X objective
lenses? - ANSWER: The slide should be close in focus after focusing at the 4x
objective. Focusing with the coarse focus knob would result in too large a change in
focus.
What is the total magnification of a specimen using the 40X objective? - ANSWER:
The total magnification would be 400x
What is the advantage of starting with the 4X objective before moving to the 10X
and then the 40X objective? - ANSWER: The lower power objective has a larger field
of view and is easier to focus.
The coarse focus knob can be used to adjust the focus when using any of the
objective lenses. - ANSWER: False
The total magnification achieved when using a 100X oil immersion lens with 10X
binocular eyepieces is ________. - ANSWER: 1000X
The total magnification when combined with the ocular lens Medium-power lens -
ANSWER: 100x
The total magnification when combined with the ocular lens High-dry lens -
ANSWER: 400x
The total magnification when combined with the ocular lens Oil immersion lens -
ANSWER: 1000x
The total magnification when combined with the ocular lens Scanning objective lens
- ANSWER: 40x
Which of the following statements are true of protozoans? - ANSWER: Most are
beneficial but a few may be pathogenic
Protozoans are prokaryotic
Protozoans can be used as bioindicators of water quality
Protozoans use several different forms of motility
Protozoans are predators of smaller organisms
Which of the following forms of motility is used by amoeba? - ANSWER: Pseudopod
FALL-SPRING FSW GRADED A+
If the 10x objective is used, what is the total magnification of the slide? (Assume the
oculars have a magnification of 10x.) - ANSWER: 100x
Which objective should be used with immersion oil? - ANSWER: The 100x objective
Why is the 40x objective cleaned before the 100x oil immersion objective? -
ANSWER: To prevent the transfer of immersion oil from the 100x to the 40x
objective
Using a microscope with 10x oculars, the total magnification of a specimen with a 4x
objective will be - ANSWER: 40x
A laboratory photo atlas contains an image with a total magnification listed as 1000x.
Using a microscope with 10x ocular magnification, which objective should be used to
recreate the image? - ANSWER: 100x objective
With which objective can you use both the course and fine focus controls? -
ANSWER: The 4x objective
Immersion oil is used with - ANSWER: objectives with magnifications of 50x or
greater
When cleaning the microscope, the ________ objective should be cleaned prior to
the _______ objective to prevent the transfer of oil between them. - ANSWER:
40x;100x
Why is it challenging to focus on bacteria in a hanging drop preparation? - ANSWER:
The bacteria in a hanging drop preparation are not stained.
Nitrogen fixation is the - ANSWER: conversion of atmospheric nitrogen to organic
forms
Chloroplasts are used to carry out ________ by algae. - ANSWER: photosynthesis
Algae are ________ and cyanobacteria are - ANSWER: eukaryotic; prokaryotic
What is important to remember when using oil immersion? - ANSWER: Immersion oil
can only be used with the 100X objective, and without it, the image is blurry.
What are advantages to using a wet mount slide preparation?Select all that apply -
ANSWER: A. There are additional advantages to viewing a living specimen.
C. There are additional advantages to viewing a motile specimen.
, D. Wet mount slide preparation allows the viewing of specimen behavior as they
interact with the environment.
Which of the three factors affecting image quality is altered by the light source? -
ANSWER: Contrast
How do the coarse and fine focus knobs work on a brightfield microscope? -
ANSWER: The focus knobs move the stage up and down
Why should the fine focus knob typically be used with the 10X and 40X objective
lenses? - ANSWER: The slide should be close in focus after focusing at the 4x
objective. Focusing with the coarse focus knob would result in too large a change in
focus.
What is the total magnification of a specimen using the 40X objective? - ANSWER:
The total magnification would be 400x
What is the advantage of starting with the 4X objective before moving to the 10X
and then the 40X objective? - ANSWER: The lower power objective has a larger field
of view and is easier to focus.
The coarse focus knob can be used to adjust the focus when using any of the
objective lenses. - ANSWER: False
The total magnification achieved when using a 100X oil immersion lens with 10X
binocular eyepieces is ________. - ANSWER: 1000X
The total magnification when combined with the ocular lens Medium-power lens -
ANSWER: 100x
The total magnification when combined with the ocular lens High-dry lens -
ANSWER: 400x
The total magnification when combined with the ocular lens Oil immersion lens -
ANSWER: 1000x
The total magnification when combined with the ocular lens Scanning objective lens
- ANSWER: 40x
Which of the following statements are true of protozoans? - ANSWER: Most are
beneficial but a few may be pathogenic
Protozoans are prokaryotic
Protozoans can be used as bioindicators of water quality
Protozoans use several different forms of motility
Protozoans are predators of smaller organisms
Which of the following forms of motility is used by amoeba? - ANSWER: Pseudopod
