BIO 3210 Midterm Exam Latest 2024.2025 100%
Accurate Fall-Spring Term Verified Exam With Answers
Embryonic stem cells are different from primary cell cultures or permanent cell lines
derived from a tissue in that they are capable of
a. generating many cell types.
b. growing and dividing.
c. synthesizing their own DNA.
d. transporting nutrients across their plasma membrane. - ANSWER: a. generating
many cell types
Solid tissues in animals include epithelial tissue, _______ tissue, nervous tissue, and
muscle.
a. blood
b. connective
c. ground
d. epidermal - ANSWER: b. connective
In fluorescence microscopy, the immediate source of the light detected is light that
has been _______ the sample.
a. absorbed by
b. emitted by
c. exciting
d. scattered from - ANSWER: b. emitted by
Approximately how many doublings can normal human fibroblasts undergo in
culture?
a. 5
b. 50 to 100
c. They will go on proliferating indefinitely.
d. They will not double at all, but dispersing them in culture allows them to be
studied in isolation from other cell types. - ANSWER: b. 50 to 100
What is the theoretical diffraction limit of resolution of a light microscope used to
look at a sample through oil?
a. 0.22 μm
b. 0.2 nm
c. 1 mm
d. 0.305 μm - ANSWER: a. 0.22 µm
Which of the following is not a unicellular eukaryote?
a. Saccharomyces cerevisiae
b. Paramecium
c. Archaebacteria
d. Chlamydomonas - ANSWER: c. Archaebacteria
,Which of the following is a high-resolution light microscope technique for detecting
interactions between proteins?
a. FRAP (fluorescence recovery after photobleaching)
b. FRET (fluorescence resonance energy transfer)
c. MPEM (multi-photon excitation microscopy)
d. Confocal microscopy - ANSWER: b. FRET (fluorescence resonance energy transfer)
The feature that most clearly separates eukaryotes from prokaryotes is the presence
of _______ in eukaryotic cells.
a. ribosomes
b. oxidative phosphorylation
c. DNA molecules
d. a nucleus - ANSWER: d. a nucleus
The original atmosphere of Earth is thought to have been rich in
a. CO2, N2, O2, Rn, and Ne.
b. CO2, N2, O2, Ar, and Ne.
c. CO2, N2, H2, H2S, and CO.
d. CO2, N2, H2, Kr, and Xe. - ANSWER: c. CO2, N2, H2, H2S, and CO
Bacteriophages are
a. a strain of gram-negative bacteria.
b. bacteria that phagocytose other competing bacteria.
c. bacterial lysosomes.
d. viruses that infect bacteria. - ANSWER: d. viruses that infect bacteria
Chloroplasts are thought to have originated from endosymbiosis of _______ by a
large host cell.
a. anaerobic bacteria
b. photosynthetic eubacteria such as cyanobacteria
c. fungi such as yeast
d. nonphotosynthetic aerobic bacteria - ANSWER: b. photosynthetic eubacteria such
as cyanobacteria
The source of the atmospheric oxygen necessary for the development of oxidative
metabolism is thought to have been
a. glycolysis.
b. the formation of Earth.
c. the breakdown of ATP.
d. photosynthesis. - ANSWER: d. photosynthesis
Which of the following are the most commonly used mammals for genetic studies?
a. Humans
b. Xenopus laevis
c. Mice
d. Cats - ANSWER: c. Mice
, The genome of eukaryotes consists of genes derived from
a. archaebacteria alone.
b. bacteria alone.
c. both archaebacteria and bacteria.
d. neither archaebacteria nor bacteria. - ANSWER: c. both archaebacteria and
bacteria
In contrast to animal cells, yeast or bacterial cell cultures can be grown on fairly
simple media without the need to add amino acids or various vitamins and
hormones. This is primarily because animal cells
a. typically live in association with other cells.
b. are less capable of transporting small molecules across their membranes.
c. come from organisms that have specialized cell types.
d. have too many genes to be able to keep track of the biosynthesis of needed
metabolites. - ANSWER: c. come from organisms that have specialized cell types
The original cell was thought to have arisen from the enclosure of self-replicating
_______ by a phospholipid membrane.
a. DNA
b. carbohydrate
c. protein
d. RNA - ANSWER: d. RNA
Transmission electron microscopy is used to
a. study in single, thin sections the shapes of whole cells.
b. view living cells in three dimensions.
c. view fluorescently labeled proteins in cells.
d. observe subcellular organelles and macromolecules. - ANSWER: d. observe
subcellular organelles and macromolecules
How many genes does an E. coli have?
a. 4,300
b. 6,000
c. 14,000
d. 20,000-25,000 - ANSWER: a. 4,300
An E. coli cell under well-defined laboratory conditions divides about every
a. 20 minutes.
b. 2 hours.
c. 12 hours.
d. 24 hours. - ANSWER: a. 20 minutes
What is the smallest number of ultracentrifugation steps needed to separate nuclei
from ribosomes in a cellular lysate?
a. 1
b. 2
c. 4
Accurate Fall-Spring Term Verified Exam With Answers
Embryonic stem cells are different from primary cell cultures or permanent cell lines
derived from a tissue in that they are capable of
a. generating many cell types.
b. growing and dividing.
c. synthesizing their own DNA.
d. transporting nutrients across their plasma membrane. - ANSWER: a. generating
many cell types
Solid tissues in animals include epithelial tissue, _______ tissue, nervous tissue, and
muscle.
a. blood
b. connective
c. ground
d. epidermal - ANSWER: b. connective
In fluorescence microscopy, the immediate source of the light detected is light that
has been _______ the sample.
a. absorbed by
b. emitted by
c. exciting
d. scattered from - ANSWER: b. emitted by
Approximately how many doublings can normal human fibroblasts undergo in
culture?
a. 5
b. 50 to 100
c. They will go on proliferating indefinitely.
d. They will not double at all, but dispersing them in culture allows them to be
studied in isolation from other cell types. - ANSWER: b. 50 to 100
What is the theoretical diffraction limit of resolution of a light microscope used to
look at a sample through oil?
a. 0.22 μm
b. 0.2 nm
c. 1 mm
d. 0.305 μm - ANSWER: a. 0.22 µm
Which of the following is not a unicellular eukaryote?
a. Saccharomyces cerevisiae
b. Paramecium
c. Archaebacteria
d. Chlamydomonas - ANSWER: c. Archaebacteria
,Which of the following is a high-resolution light microscope technique for detecting
interactions between proteins?
a. FRAP (fluorescence recovery after photobleaching)
b. FRET (fluorescence resonance energy transfer)
c. MPEM (multi-photon excitation microscopy)
d. Confocal microscopy - ANSWER: b. FRET (fluorescence resonance energy transfer)
The feature that most clearly separates eukaryotes from prokaryotes is the presence
of _______ in eukaryotic cells.
a. ribosomes
b. oxidative phosphorylation
c. DNA molecules
d. a nucleus - ANSWER: d. a nucleus
The original atmosphere of Earth is thought to have been rich in
a. CO2, N2, O2, Rn, and Ne.
b. CO2, N2, O2, Ar, and Ne.
c. CO2, N2, H2, H2S, and CO.
d. CO2, N2, H2, Kr, and Xe. - ANSWER: c. CO2, N2, H2, H2S, and CO
Bacteriophages are
a. a strain of gram-negative bacteria.
b. bacteria that phagocytose other competing bacteria.
c. bacterial lysosomes.
d. viruses that infect bacteria. - ANSWER: d. viruses that infect bacteria
Chloroplasts are thought to have originated from endosymbiosis of _______ by a
large host cell.
a. anaerobic bacteria
b. photosynthetic eubacteria such as cyanobacteria
c. fungi such as yeast
d. nonphotosynthetic aerobic bacteria - ANSWER: b. photosynthetic eubacteria such
as cyanobacteria
The source of the atmospheric oxygen necessary for the development of oxidative
metabolism is thought to have been
a. glycolysis.
b. the formation of Earth.
c. the breakdown of ATP.
d. photosynthesis. - ANSWER: d. photosynthesis
Which of the following are the most commonly used mammals for genetic studies?
a. Humans
b. Xenopus laevis
c. Mice
d. Cats - ANSWER: c. Mice
, The genome of eukaryotes consists of genes derived from
a. archaebacteria alone.
b. bacteria alone.
c. both archaebacteria and bacteria.
d. neither archaebacteria nor bacteria. - ANSWER: c. both archaebacteria and
bacteria
In contrast to animal cells, yeast or bacterial cell cultures can be grown on fairly
simple media without the need to add amino acids or various vitamins and
hormones. This is primarily because animal cells
a. typically live in association with other cells.
b. are less capable of transporting small molecules across their membranes.
c. come from organisms that have specialized cell types.
d. have too many genes to be able to keep track of the biosynthesis of needed
metabolites. - ANSWER: c. come from organisms that have specialized cell types
The original cell was thought to have arisen from the enclosure of self-replicating
_______ by a phospholipid membrane.
a. DNA
b. carbohydrate
c. protein
d. RNA - ANSWER: d. RNA
Transmission electron microscopy is used to
a. study in single, thin sections the shapes of whole cells.
b. view living cells in three dimensions.
c. view fluorescently labeled proteins in cells.
d. observe subcellular organelles and macromolecules. - ANSWER: d. observe
subcellular organelles and macromolecules
How many genes does an E. coli have?
a. 4,300
b. 6,000
c. 14,000
d. 20,000-25,000 - ANSWER: a. 4,300
An E. coli cell under well-defined laboratory conditions divides about every
a. 20 minutes.
b. 2 hours.
c. 12 hours.
d. 24 hours. - ANSWER: a. 20 minutes
What is the smallest number of ultracentrifugation steps needed to separate nuclei
from ribosomes in a cellular lysate?
a. 1
b. 2
c. 4
