Growth Stoichiometry:
Growth stoichiometry involves the quantitative relationship between the inputs
(nutrients) and outputs (biomass, by-products) in biological growth processes.
Biomass Yield (Y): The amount of biomass produced per unit of substrate
consumed, typically expressed in grams of biomass per gram of substrate (g/g).
Specific Growth Rate (μ): The rate at which biomass increases, often
expressed in units of time (e.g., h⁻¹).
Biomass Produced =biomass yield coeff × Substrate Consumed
CELL GROWTH OBEYS: The Law of Conservation of Matter is a fundamental principle in
chemistry and physics stating that matter cannot be created or destroyed in an isolated system. This
means that the total mass of substances involved in a chemical reaction remains constant before and
after the reaction
MASS CONSERVATION : The mass of reactants (starting materials) equals the mass of products
(resulting materials) in a chemical reaction. This implies that the amount of matter remains
unchanged. The law implies that all chemical equations must be balanced to reflect the conservation
of mass. Each element must have the same number of atoms on both sides of the equation.
RESPIRATORY QUOTIENT: ratio of carbon dioxide (CO₂) produced to oxygen
(O₂) consumed.=== COEFF OF CO2 / COEFF OF 02 ( in reactions)
Different substrates yield different amounts of CO₂ for the same amount of O₂
consumed.. The complete oxidation of glucose (C₆H₁₂O₆) yields an RQ of 1.0.
DEGREE OF REDUCTION:
Higher Values of DR: Indicate that the compound is more reduced, suggesting a higher
energy content per mole. Lower Values of DR: Indicate that the compound is less reduced, implying
lower energy content. Compounds like carbon dioxide (CO₂) have a degree of reduction close to zero.
NO OF AVAILABLE ELECTRONS IN BIOMASS+ NO OF AVAILABLE
ELECTRONS IN PRODUCT == NO OF ELECTRONS AVAILABLE IN SUBSTRATE
+ NO OF ELECTRONS AVAILABLE IN OXYGEN
BIOMASS YIELD:
=measures the efficiency with which a substrate is converted into
biomass
Factors Affecting Biomass Yield
1. Type of Microorganism:Different microorganisms have varying efficiencies in biomass
production based on their metabolic pathways.
2. Substrate Type:The nature and composition of the substrate influence how effectively it can
be converted to biomass.
Growth stoichiometry involves the quantitative relationship between the inputs
(nutrients) and outputs (biomass, by-products) in biological growth processes.
Biomass Yield (Y): The amount of biomass produced per unit of substrate
consumed, typically expressed in grams of biomass per gram of substrate (g/g).
Specific Growth Rate (μ): The rate at which biomass increases, often
expressed in units of time (e.g., h⁻¹).
Biomass Produced =biomass yield coeff × Substrate Consumed
CELL GROWTH OBEYS: The Law of Conservation of Matter is a fundamental principle in
chemistry and physics stating that matter cannot be created or destroyed in an isolated system. This
means that the total mass of substances involved in a chemical reaction remains constant before and
after the reaction
MASS CONSERVATION : The mass of reactants (starting materials) equals the mass of products
(resulting materials) in a chemical reaction. This implies that the amount of matter remains
unchanged. The law implies that all chemical equations must be balanced to reflect the conservation
of mass. Each element must have the same number of atoms on both sides of the equation.
RESPIRATORY QUOTIENT: ratio of carbon dioxide (CO₂) produced to oxygen
(O₂) consumed.=== COEFF OF CO2 / COEFF OF 02 ( in reactions)
Different substrates yield different amounts of CO₂ for the same amount of O₂
consumed.. The complete oxidation of glucose (C₆H₁₂O₆) yields an RQ of 1.0.
DEGREE OF REDUCTION:
Higher Values of DR: Indicate that the compound is more reduced, suggesting a higher
energy content per mole. Lower Values of DR: Indicate that the compound is less reduced, implying
lower energy content. Compounds like carbon dioxide (CO₂) have a degree of reduction close to zero.
NO OF AVAILABLE ELECTRONS IN BIOMASS+ NO OF AVAILABLE
ELECTRONS IN PRODUCT == NO OF ELECTRONS AVAILABLE IN SUBSTRATE
+ NO OF ELECTRONS AVAILABLE IN OXYGEN
BIOMASS YIELD:
=measures the efficiency with which a substrate is converted into
biomass
Factors Affecting Biomass Yield
1. Type of Microorganism:Different microorganisms have varying efficiencies in biomass
production based on their metabolic pathways.
2. Substrate Type:The nature and composition of the substrate influence how effectively it can
be converted to biomass.
