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different levels of cleanliness
sterilization, disinfection, sanitization
common situations for sterilization
biohazardous material must be sterilized prior to discarding it to avoid introducing
potential infectious hazards at the landfill; materials/instruments must be sterile prior to
use, ensures accuracy and meaningful info to use in diagnosis and treatment of patient;
supplies/equipment used directly on a patient for invasive procedures to prevent direct
transmission of infectious organisms
forms of physical sterilization
moist heat and dry heat
moist heat
uses water vapour to kill organisms by denaturing their proteins
steam autoclave
instrument to sterilize with moist heat; steam pressure to reach temp of 121ºC; all
organisms including spored are destroyed
dry heat
effective alternative for sterilizing materials before disposal
incineration
used for dry heat sterilization in hospitals
alkylating agents
most effective form of chemical sterilization; used for materials such as plastic (which
would be destroyed by heat)
ethylene oxide
sterilization agent for items that are damaged by heat sterilization; gas at room temp;
used in gas autoclave
gas autoclave
most effective non-heat sterilizer of heat sensitive materials
glutataldehyde
alkylating agent; uses 2.0% solution to sterilize or disinfect; level of cleanliness depends
on length of exposure; used for sterilizing large surfaces or other materials that can't be
autoclaved; in some spill kits
phenolics
disinfectant; derivatives of phenol and are less toxic and more effective; don't destroy all
organisms, but effective against most
catonic detergents
disinfectant; "quaternary ammonium compounds"; used to clean; limited effect on
organisms
alcohols
disinfectant or antiseptic; most effective are propanol and isopropanol (concentration of
70%); used as antiseptic on skin before phlebotomy; can be used for cleaning
tincture