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Disinfection
destruction of vegetative pathogens;
NONLIVING OBJECTS
Antiseptic
agent which prevents bact. contamination and growth;
LIVING TISSUE
Which agent doesn't necessarily work better at higher concentrations?
Alcohol (60%)
Factors of disinfectant activity
Organic material
Temp (higher = more effective)
Contact time (longer = more effective)
What 3 organisms are used in the AOAC Use-Dilution test?
-Staphylococcus aureus
-Pseudomonas aeruginosa
-Salmonella choleraesuis
How to compute D values (decimal reduction time) -
-Time required to kill 90% (1 log) at a specific temp
Ionizing radiation
- Shorter wavelength (<1nm)
- Higher energy
- Better penetration
- Sterilizes
- Electron beams, gamma, xray
Antimicrobial handwash
4% chlorhexidine
Glutaraldehyde is _________ to inactivation by organic material
a) most resistant
b) least resistant
most resistant
HPV is resistant to __________ by most disinfectants
a) inactivation
b) activation
inactivation
instrument disinfection
Peracetic acid
Counter tops or surfaces
70-80% ethanol/ethyl alcohol
ethanol on counter tops is NOT better at high concentrations
true
Mop water/clean floors
, substituted phenols
chlorine/chlorine-based products =
___________ by organic material
a) activated
b) deactivated
deactivated
Sterilization of plastics
Gamma irradiation
Chemotherapeutic drug:
Anti-tuberculosis drug
Inhibits cell wall synthesis.
Isoniazid
Chemotherapeutic drug:
Combined with trimethoprim
Inhibits dihydrofolate synthesis.
Sulfanilamide
Chemotherapeutic drug:
A beta-lactam antibiotic
Cephalosporin
Chemotherapeutic drug:
Aminoglycoside;
Injection
Streptomycin
Chemotherapeutic drug:
The first antibiotic used in humans.
Penicillin G
Chemotherapeutic drug:
Macrolides
Binds the 50S ribosomal subunit
Erythromycin
Chemotherapeutic drug:
Inhibits bacterial DNA gyrase
Quinolone
Chemotherapeutic drug:
Found in topical ointment
Kills GAS
Bacitracin