MMBIO 221 TEST QUESTIONS AND ANSWERS A+
GRADED. Buy Quality Materials!
Antimicrobial handwash
4% chlorhexidine
semi-critical instrument disinfection
peracetic acid or H2O2 based agents
countertop
70-80% ethanol
antimicrobial in mop water
substituted phenols
stabilization of plastic
gamma radiation
Anti- TB (inhibit cell wall synthesis)
Isoniazid
combined with trimethoprim (inhibit dihydrofolate synthesis)
sulfanilamide
B lactam w 1,2,3
cephalosporin
aminoglycoside (mech. of action and injected?)
streptomycin. binds 30S ribosomal subunit. high concentration
first antibiotic
penicillin
macrolides (and mech of action)
erythromycin. binds 50S ribosomal subunit
bacterial DNA gyrase (disrupts nucleic acid synthesis)
quinolones
kills S. pyo/group A, found in topical ointments
bacitracin. neosporin
antifungal
amphotericin B
herpes
acyclovir
black hairy tongue
metronidazole
a quinolone that inhibits prokaryotic DNA gyrase
ciproflaxacin
Inhibits DNA directed RNA polymerase. used to treat TB
rifampin
most common/produced
cephalosporin
HIV
AZT
IgM
, pentomer, first produced--> primary response. fixes complement
IgD
never secreted, BCR of naive (unactivated B cells), monomer
IgG
monomer, most common in blood serum, cross placenta, best at activating complement
IgE
monomer, most in allergic/parasitc infections, histamine release
IgA
dimer, mucous membranes, tears/milk, most produced per day. J chain.
plasma
fluid blood WITH clotting
serum
fluid blood WITHOUT clotting
Immuno-electrophoresis
visualize/see thickness of proteins (Ig, cytokines)
hemagglutination
blood typing
fluorescent Ab test
fluorescent labels to tag proteins. UV light source
Ouchterlony
double diffusion, test for Ab presence
Western Blot
visualize proteins, nitrocellulose membrane. detect proteins on a membrane
ELISA
HIV tester, enzymatic reaction = color change
Radial immunodiffusion
test for Ab amount
agglutination
clumping of INSOLUBLE particles/antigen
precipitation
clumping of SOLUBLE partcles/antigen
Type 1 Hypersensitivity
allergies (basophils, mast cells), IgE antibody, immediate response. Hay fever. asthma
Type 2 Hypersensitivity
transfusion reaction or hemolytic disease in newborn. RBC lysis. drug induced anemia
Type 3 Hypersensitivity
immune complexes (antibody-antigen), gets stuck in vessel walls, call in neutrophils-->
lots of inflammation/damage. Rheumatoid arthritis
Type 4 hypersensitivity
delayed onset, T cell mediated, poison ivy or TB testing. allergic contact dermatitis to
latex gloves
difference between agglutination and precipitation
solubility of antigen!
autograft
tissue to same organism
isografts
GRADED. Buy Quality Materials!
Antimicrobial handwash
4% chlorhexidine
semi-critical instrument disinfection
peracetic acid or H2O2 based agents
countertop
70-80% ethanol
antimicrobial in mop water
substituted phenols
stabilization of plastic
gamma radiation
Anti- TB (inhibit cell wall synthesis)
Isoniazid
combined with trimethoprim (inhibit dihydrofolate synthesis)
sulfanilamide
B lactam w 1,2,3
cephalosporin
aminoglycoside (mech. of action and injected?)
streptomycin. binds 30S ribosomal subunit. high concentration
first antibiotic
penicillin
macrolides (and mech of action)
erythromycin. binds 50S ribosomal subunit
bacterial DNA gyrase (disrupts nucleic acid synthesis)
quinolones
kills S. pyo/group A, found in topical ointments
bacitracin. neosporin
antifungal
amphotericin B
herpes
acyclovir
black hairy tongue
metronidazole
a quinolone that inhibits prokaryotic DNA gyrase
ciproflaxacin
Inhibits DNA directed RNA polymerase. used to treat TB
rifampin
most common/produced
cephalosporin
HIV
AZT
IgM
, pentomer, first produced--> primary response. fixes complement
IgD
never secreted, BCR of naive (unactivated B cells), monomer
IgG
monomer, most common in blood serum, cross placenta, best at activating complement
IgE
monomer, most in allergic/parasitc infections, histamine release
IgA
dimer, mucous membranes, tears/milk, most produced per day. J chain.
plasma
fluid blood WITH clotting
serum
fluid blood WITHOUT clotting
Immuno-electrophoresis
visualize/see thickness of proteins (Ig, cytokines)
hemagglutination
blood typing
fluorescent Ab test
fluorescent labels to tag proteins. UV light source
Ouchterlony
double diffusion, test for Ab presence
Western Blot
visualize proteins, nitrocellulose membrane. detect proteins on a membrane
ELISA
HIV tester, enzymatic reaction = color change
Radial immunodiffusion
test for Ab amount
agglutination
clumping of INSOLUBLE particles/antigen
precipitation
clumping of SOLUBLE partcles/antigen
Type 1 Hypersensitivity
allergies (basophils, mast cells), IgE antibody, immediate response. Hay fever. asthma
Type 2 Hypersensitivity
transfusion reaction or hemolytic disease in newborn. RBC lysis. drug induced anemia
Type 3 Hypersensitivity
immune complexes (antibody-antigen), gets stuck in vessel walls, call in neutrophils-->
lots of inflammation/damage. Rheumatoid arthritis
Type 4 hypersensitivity
delayed onset, T cell mediated, poison ivy or TB testing. allergic contact dermatitis to
latex gloves
difference between agglutination and precipitation
solubility of antigen!
autograft
tissue to same organism
isografts
